Overview

  • Product name

    Anti-MMP13 antibody [EP1263Y]
    See all MMP13 primary antibodies
  • Description

    Rabbit monoclonal [EP1263Y] to MMP13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MMP13 (C terminal). The exact sequence is proprietary.

  • Positive control

  • General notes

    Rat and mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab51072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

For unpurified use at 1/500

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP.
  • Target

    • Function

      Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
    • Tissue specificity

      Seems to be specific to breast carcinomas.
    • Involvement in disease

      Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
      Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
    • Sequence similarities

      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • Domain

      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • CLG 3 antibody
      • CLG3 antibody
      • Collagenase 3 antibody
      • Collagenase3 antibody
      • MANDP1 antibody
      • Matrix metallopeptidase 13 (collagenase 3) antibody
      • Matrix Metalloproteinase 13 antibody
      • Matrix metalloproteinase-13 antibody
      • MMP 13 antibody
      • MMP-13 antibody
      • Mmp13 antibody
      • MMP13_HUMAN antibody
      see all

    Images

    • Anti-MMP13 antibody [EP1263Y] (ab51072) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 54 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Son YO  et al. RNA-binding protein ZFP36L1 regulates osteoarthritis by modulating members of the heat shock protein 70 family. Nat Commun 10:77 (2019). Read more (PubMed: 30622281) »
    • Kang LJ  et al. 3'-Sialyllactose as an inhibitor of p65 phosphorylation ameliorates the progression of experimental rheumatoid arthritis. Br J Pharmacol 175:4295-4309 (2018). Read more (PubMed: 30152858) »
    See all 20 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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