Product nameAnti-MMP14 antibody
See all MMP14 primary antibodies
DescriptionRabbit polyclonal to MMP14
Tested applicationsSuitable for: Flow Cyt, IHC-P, IHC-Fr, WB, ICC, ICC/IF, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human MMP14 aa 150-250. The exact sequence is proprietary.
Database link: P50281
- IHC-P: Human colon tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab3644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 19910495
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|ICC||Use a concentration of 1 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IP||Use at an assay dependent concentration. PubMed: 22002060|
FunctionSeems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
Tissue specificityExpressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsThe precursor is cleaved by a furin endopeptidase.
Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Matrix metallopeptidase 14 (membrane inserted) antibody
- Matrix metalloproteinase 14 antibody
- Matrix metalloproteinase-14 antibody
All lanes : Anti-MMP14 antibody (ab3644) at 1/500 dilution
Lane 1 : 15ug MCF-7 whole cell lysate
Lane 2 : 15ug MSTO-2H11 whole cell lysate
Lane 3 : 15ug Malme-3M whole cell lysate
Lane 4 : 15ug Calu-1 whole cell lysate
Lane 5 : 15ug Sk-Lu-1 whole cell lysate
All lanes : Peroxidase-conjugated affinipure goat anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 54,66 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa (possible non-specific binding), 98 kDa (possible non-specific binding)
Exposure time: 30 seconds
This image is courtesy of an anonymous Abreview
ICC/IF image of ab3644 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3644, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab3644 staining in Human Colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3644, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Neuhaus J et al. Protease Expression Levels in Prostate Cancer Tissue Can Explain Prostate Cancer-Associated Seminal Biomarkers-An Explorative Concept Study. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 28471417) »
- Eiseler T et al. Protein Kinase D2 Assembles a Multiprotein Complex at the Trans-Golgi Network to Regulate Matrix Metalloproteinase Secretion. J Biol Chem 291:462-77 (2016). Read more (PubMed: 26507660) »