Product nameAnti-MMP14 antibody
See all MMP14 primary antibodies
DescriptionRabbit polyclonal to MMP14
Specificityab53712 detects endogenous levels of total MMP14 protein
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, ELISAmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human MMP14 aa 471-520 (C terminal).
Database link: P50281
- IHC-P: Human breast carcinoma tissue. WB: K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab53712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 23326364|
|WB||1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionSeems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
Tissue specificityExpressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsThe precursor is cleaved by a furin endopeptidase.
Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Matrix metallopeptidase 14 (membrane inserted) antibody
- Matrix metalloproteinase 14 antibody
- Matrix metalloproteinase-14 antibody
All lanes : Anti-MMP14 antibody (ab53712) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
ab53712, at a 1/50 dilution, minus immunising peptide, staining Human MMP14 in Breast Carcinoma, using Immunohistochemsitry, Paraffin embedded tissue.
ab53712, at a 1/50 dilution, plus immunising peptide, staining Human MMP14 in Breast Carcinoma, using Immunohistochemstry, Paraffin embedded tissue.
ab53712 has been referenced in 20 publications.
- Wan G et al. SLFN5 suppresses cancer cell migration and invasion by inhibiting MT1-MMP expression via AKT/GSK-3ß/ß-catenin pathway. Cell Signal 59:1-12 (2019). PubMed: 30844429
- El Hajj EC et al. Inhibitor of lysyl oxidase improves cardiac function and the collagen/MMP profile in response to volume overload. Am J Physiol Heart Circ Physiol 315:H463-H473 (2018). PubMed: 29775412
- Saidi D et al. Glioma-induced SIRT1-dependent activation of hMOF histone H4 lysine 16 acetyltransferase in microglia promotes a tumor supporting phenotype. Oncoimmunology 7:e1382790 (2018). PubMed: 29308302
- Mazzoni TS et al. Action of the Metalloproteinases in Gonadal Remodeling during Sex Reversal in the Sequential Hermaphroditism of the Teleostei Fish Synbranchus marmoratus (Synbranchiformes: Synbranchidae). Cells 7:N/A (2018). PubMed: 29695033
- Abu El-Asrar AM et al. Matrix metalloproteinase-14 is a biomarker of angiogenic activity in proliferative diabetic retinopathy. Mol Vis 24:394-406 (2018). PubMed: 29853773