• Product name
  • Description
    Rabbit polyclonal to MMP14
  • Host species
  • Specificity
    ab53712 detects endogenous levels of total MMP14 protein
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MMP14 aa 471-520 (C terminal).
    Database link: P50281

  • Positive control
    • IHC-P: Human breast carcinoma tissue. WB: K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate.



Our Abpromise guarantee covers the use of ab53712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23326364
WB 1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/5000.


  • Function
    Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
  • Tissue specificity
    Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization
    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Matrix metallopeptidase 14 (membrane inserted) antibody
    • Matrix metalloproteinase 14 antibody
    • Matrix metalloproteinase-14 antibody
    • Membrane type 1 matrix metalloproteinase antibody
    • Membrane type 1 metalloprotease antibody
    • Membrane type matrix metalloproteinase 1 antibody
    • Membrane-type matrix metalloproteinase 1 antibody
    • Membrane-type-1 matrix metalloproteinase antibody
    • MMP 14 antibody
    • MMP X1 antibody
    • MMP-14 antibody
    • MMP-X1 antibody
    • Mmp14 antibody
    • MMP14_HUMAN antibody
    • MMPX1 antibody
    • MT MMP 1 antibody
    • MT-MMP 1 antibody
    • MT1 MMP antibody
    • MT1-MMP antibody
    • MT1MMP antibody
    • MTMMP 1 antibody
    • MTMMP1 antibody
    see all


  • All lanes : Anti-MMP14 antibody (ab53712) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa
    Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.

  • ab53712, at a 1/50 dilution, minus immunising peptide, staining Human MMP14 in Breast Carcinoma, using Immunohistochemsitry, Paraffin embedded tissue.
  • ab53712, at a 1/50 dilution, plus immunising peptide, staining Human MMP14 in Breast Carcinoma, using Immunohistochemstry, Paraffin embedded tissue.


This product has been referenced in:
  • Saidi D  et al. Glioma-induced SIRT1-dependent activation of hMOF histone H4 lysine 16 acetyltransferase in microglia promotes a tumor supporting phenotype. Oncoimmunology 7:e1382790 (2018). Read more (PubMed: 29308302) »
  • Mazzoni TS  et al. Action of the Metalloproteinases in Gonadal Remodeling during Sex Reversal in the Sequential Hermaphroditism of the Teleostei Fish Synbranchus marmoratus (Synbranchiformes: Synbranchidae). Cells 7:N/A (2018). Read more (PubMed: 29695033) »
See all 17 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Western blot
Rat Cell lysate - whole cell (rat vascular smooth muscle cells)
Loading amount
100000 cells
rat vascular smooth muscle cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Feb 05 2013


Thank you for your inquiry.

Please find the SDS in the attachment. The SDS can now be downloaded also from the datasheet directly. Please follow this link: https://www.abcam.com/index.html?datasheet=53712 (or use the following: https://www.abcam.com/index.html?datasheet=53712).

I hope this information is helpful. Please do not hesitate to contact us again, should you need any more information.

Read More


Thank you for your inquiry.

The antibody you mentioned is a polyclonal antibody. Polyclonal antibodies consist of a mixed population of IgGs each of which will recognize a different epitope. Therefore for polyclonal antibodies the epitopes are not mapped - only for monoclonal antibodies.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any additional information or advice.

Read More


Thank you for contacting us.

I am sorry to hear that you are encountering problems with these antibodies in WB.

I agree thatab38898 is giving you poor results, and this could be a due to a faulty vial or lot.

As for the MMP14 antibody ab53712, the MW of the unprocessed precursor is 66 kDa (see the database entry for the human protein: http://www.uniprot.org/uniprot/P50281), but the protein contains a signal peptide as well as a propeptide. Thus, the mature MMP14 protein has only 471 residues which would give you a band shorter than 66 kDa. Therefore, I believe the results you are getting make sense as you see a weak band around 60 kDa (signal peptide is lacking) and a strong band around 52 kDa (mature protein without signal peptide and propeptide).

Could you please let me know the following experimental details for both antibodies:

1) What antibody dilutionand incubation time was used?
2) Which blocking reagent was employed?
3) Is the secondary antibody working well otherwise?

Please let me know also your order or PO number. If ab38898 was purchased within the last 6 months I'd be happy to offer you a free of charge replacement, credit or refund.

As for ab53712, I think you are detecting the correct protein and the antibody is fine. Please let me know your thoughts in that regard.

I look forward to hear back from you and assist you further.

Read More
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (lymphoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer (pH 6.0)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 12 2010

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