Anti-MMP14 antibody [EP1264Y] - Low endotoxin, Azide free (ab168726)
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Overview
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Product nameAnti-MMP14 antibody [EP1264Y] - Low endotoxin, Azide free
See all MMP14 primary antibodies -
DescriptionRabbit monoclonal [EP1264Y] to MMP14 - Low endotoxin, Azide free
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Host speciesRabbit
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Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, IHC-P, IPmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to Human MMP14.
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Positive control
- WB: recombinant protein
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General notes
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEP1264Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab168726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 65 kDa. | |
| IHC-P | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. |
Target
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FunctionSeems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
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Tissue specificityExpressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
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Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
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Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Database links
- Entrez Gene: 4323 Human
- Entrez Gene: 17387 Mouse
- Entrez Gene: 81707 Rat
- Omim: 600754 Human
- SwissProt: P50281 Human
- SwissProt: P53690 Mouse
- SwissProt: Q10739 Rat
- Unigene: 2399 Human
see all -
Alternative names
- Matrix metallopeptidase 14 (membrane inserted) antibody
- Matrix metalloproteinase 14 antibody
- Matrix metalloproteinase-14 antibody
see all
Images
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![Flow Cytometry - Anti-MMP14 antibody [EP1264Y] - Low endotoxin, Azide free (ab168726)](https://www.abcam.com/ps/products/168/ab168726/Images/ab168726-330774-ab51074314154ab51074FC.jpg)
Flow Cytometry - Anti-MMP14 antibody [EP1264Y] - Low endotoxin, Azide free (ab168726)Flow cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell, Left) / HT-1080 (Human fibrosarcoma epithelial cell, Right) cells labeling MMP14 with ab51074 at 1/200 dilution (0.1 μg) (red). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Rabbit monoclonal IgG (ab172730) / black was used as the isotype control. Cells incubated with secondary antibody only (blue) was used as the unlabeled control. Gated on viable cells.
Positive control (Right panel): HT-1080 cells.
Negative control (Left panel): MCF7 cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-MMP14 antibody [EP1264Y] - Low endotoxin, Azide free (ab168726)](https://www.abcam.com/ps/products/168/ab168726/Images/ab168726-330773-ab51074314152ab51074ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MMP14 antibody [EP1264Y] - Low endotoxin, Azide free (ab168726)Ab51074 staining MMP14 in HT-1080 (human fibrosarcoma epithelial cell) cells by ICC/IF (Immunocytochemistry/Immunofluorescence).
Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at 1/1000 dilution (0.2 μg/ml). An Alexa Fluor® 488 Goat anti-rabbit (ab150077) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594, ab195889) was used as the counterstain antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic and weakly membranous staining in HT-1080 cell line.
Negative control (bottom panels): MCF7 PMID: 19208838.
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![Immunoprecipitation - Anti-MMP14 antibody [EP1264Y] - Low endotoxin, Azide free (ab168726)](https://www.abcam.com/ps/products/168/ab168726/Images/ab168726-3-ab51074274271ab51074IP1.jpg)
Immunoprecipitation - Anti-MMP14 antibody [EP1264Y] - Low endotoxin, Azide free (ab168726)ab51074 (purified) at 1/20 dilution (2 µg) immunoprecipitating MMP14 in A431 (human epidermoid carcinoma) whole cell lysate.
Lane 1: A431 whole cell lysate 10ug
Lane 2: ab51074 + A431 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab51074 in A431 whole cell lysateFor western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/1000).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51074).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] - Low endotoxin, Azide free (ab168726)](https://www.abcam.com/ps/products/168/ab168726/Images/ab168726-2-ab51074274270ab51074IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] - Low endotoxin, Azide free (ab168726)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium carcinoma tissue sections labeling MMP14 with purified ab51074 at 1/100 dilution (1.7 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counterstain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution.
PBS instead of the primary antibody was used as the negative control (inset).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51074).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] - Low endotoxin, Azide free (ab168726)](https://www.abcam.com/ps/products/168/ab168726/Images/ab168726-1-ab510741.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] - Low endotoxin, Azide free (ab168726)This image is courtesy of an anonymous Abreview.ab51074 (unpurified) at 1/500 staining human kidney tissue sections by IHC-P.
The tissue was formaldehyde fixed and a heat mediated antigen retrieval step (in Tris/EDTA) was performed. The tissue was then blocked with serum and incubated with the primary antibody. A biotinylated donkey anti-rabbit IgG was used as the secondary.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51074).
Datasheets and documents
References
ab168726 has not yet been referenced specifically in any publications.
