Anti-MMP14 antibody - Hinge region (ab38971)


  • Product name
    Anti-MMP14 antibody - Hinge region
    See all MMP14 primary antibodies
  • Description
    Rabbit polyclonal to MMP14 - Hinge region
  • Host species
  • Specificity
    This antibody binds to MMP14, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9).
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Human MMP14.
    (Peptide available as ab41091)

  • Positive control
    • HT1080 cells



Our Abpromise guarantee covers the use of ab38971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB 1/2000 - 1/5000. Predicted molecular weight: 66 kDa. When used against the reduced protein this antibody identifies bands at 65 Kd and 63 Kd (the pro-form and active form) as well as activation/breakdown products.
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.


  • Function
    Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
  • Tissue specificity
    Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization
    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Matrix metallopeptidase 14 (membrane inserted) antibody
    • Matrix metalloproteinase 14 antibody
    • Matrix metalloproteinase-14 antibody
    • Membrane type 1 matrix metalloproteinase antibody
    • Membrane type 1 metalloprotease antibody
    • Membrane type matrix metalloproteinase 1 antibody
    • Membrane-type matrix metalloproteinase 1 antibody
    • Membrane-type-1 matrix metalloproteinase antibody
    • MMP 14 antibody
    • MMP X1 antibody
    • MMP-14 antibody
    • MMP-X1 antibody
    • Mmp14 antibody
    • MMP14_HUMAN antibody
    • MMPX1 antibody
    • MT MMP 1 antibody
    • MT-MMP 1 antibody
    • MT1 MMP antibody
    • MT1-MMP antibody
    • MT1MMP antibody
    • MTMMP 1 antibody
    • MTMMP1 antibody
    see all


  • All lanes : Anti-MMP14 antibody - Hinge region (ab38971)

    Lane 1 : Cell lysates from human dermal fibroblasts neonatal (no treatment)
    Lane 2 : Cell lysates from human dermal fibroblasts neonatal (treated with II1beta)

    Predicted band size: 66 kDa
    Observed band size: 66 kDa

  • Anti-MMP14 antibody - Hinge region (ab38971) at 1/1000 dilution + HT1080 whole cell lysate at 30 µg

    HRP-conjugated donkey anti-rabbit

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 64 kDa (why is the actual band size different from the predicted?)

    Exposure time: 1 minute

    See Abreview

  • All lanes : Anti-MMP14 antibody - Hinge region (ab38971) at 1/5000 dilution

    Lane 1 : Whole cell lysate prepared from HT1080 cells
    Lane 2 : Whole cell lysate prepared from human vascular smooth muscle cells

    Lysates/proteins at 5 µg per lane.

    All lanes : HRP conjugated donkey anti-rabbit IgG at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 63,65 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 20 kDa (possible non-specific binding)

    Exposure time: 30 seconds

    Blocked using 5% milk for 1 hour at 20°C.

    See Abreview

  • ab38971 at 10ug/ml staining human HT1080 cells by ICC/IF. The cells were paraformaldhyde fixed and incubated with the antibody for 1 hour. A FITC conjugated donkey anti-rabbit antibody was used as the secondary.

    See Abreview

  • Ab38971 staining Human normal myometrium tissue. Staining is localized to nuclear and cytoplasmic compartment.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 (ab64214) in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification ma


This product has been referenced in:
  • Almalki SG  et al. MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. Stem Cells Transl Med 6:1385-1398 (2017). WB ; Pig . Read more (PubMed: 28213979) »
  • Dole NS  et al. Osteocyte-Intrinsic TGF-ß Signaling Regulates Bone Quality through Perilacunar/Canalicular Remodeling. Cell Rep 21:2585-2596 (2017). IHC-P ; Mouse . Read more (PubMed: 29186693) »

See all 27 Publications for this product

Customer reviews and Q&As

Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products.
I have been in contact with the lab who have agreed to send us a vial from their new lot free of charge. I am forwarding these details...

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Thank you for taking time to contact us. I am sorry to hear this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality...

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Thanks for the extra information. I have sent you ab58815 (order number ********) so that you can have another try at getting staining in the invadopodia. Since the antibodies that we have previously sent, have been giving staining (just not in the inv...

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If you do want to test another MMP14 antibody then ab58815 would be a good bet, also ab78738 would be a possible candidate. I have been looking back at the previous MMP14 antibodies you have tried, these are ab38971, ab3644 and ab73879 and all ...

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Thank you for your reply. If the replacement antibody fails to work then I would be happy to send you another MMP14 antibody to try, however if you wish to try a completely different antibody, then I can refund the original purchase and then you can bu...

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Thank you for your reply. If the replacement antibody fails to work as stated in the datasheet, then I would be happy to refund the cost of the original antibody. Please let me know how the alternative methods work out for you and if th...

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Thank you for contacting us. According to the alignment information that you have sent to me it looks like you should have success with using these antibodies with canine protein. I hope this information is helpful to you. Please do not hesitate to con...

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Thank you for your enquiry. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instr...

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The replacement antibody ab73897 should be shipped today, Nov 9th. The fedex tracking number is #******.

I have processed your request to receive ab73897 as a replacement for ab3644. I hope that this antibody proves to be successful in your IF staining. Your new order number is *******.

1-10 of 18 Abreviews or Q&A


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