Overview

  • Product name
    Anti-MMP14 antibody - Hinge region
    See all MMP14 primary antibodies
  • Description
    Rabbit polyclonal to MMP14 - Hinge region
  • Host species
    Rabbit
  • Specificity
    This antibody binds to MMP14, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9).
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Human MMP14.
    (Peptide available as ab41091)

  • Positive control
    • HT1080 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab38971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB 1/2000 - 1/5000. Predicted molecular weight: 66 kDa. When used against the reduced protein this antibody identifies bands at 65 Kd and 63 Kd (the pro-form and active form) as well as activation/breakdown products.
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function
    Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
  • Tissue specificity
    Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization
    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Matrix metallopeptidase 14 (membrane inserted) antibody
    • Matrix metalloproteinase 14 antibody
    • Matrix metalloproteinase-14 antibody
    • Membrane type 1 matrix metalloproteinase antibody
    • Membrane type 1 metalloprotease antibody
    • Membrane type matrix metalloproteinase 1 antibody
    • Membrane-type matrix metalloproteinase 1 antibody
    • Membrane-type-1 matrix metalloproteinase antibody
    • MMP 14 antibody
    • MMP X1 antibody
    • MMP-14 antibody
    • MMP-X1 antibody
    • Mmp14 antibody
    • MMP14_HUMAN antibody
    • MMPX1 antibody
    • MT MMP 1 antibody
    • MT-MMP 1 antibody
    • MT1 MMP antibody
    • MT1-MMP antibody
    • MT1MMP antibody
    • MTMMP 1 antibody
    • MTMMP1 antibody
    see all

Images

  • All lanes : Anti-MMP14 antibody - Hinge region (ab38971)

    Lane 1 : Cell lysates from human dermal fibroblasts neonatal (no treatment)
    Lane 2 : Cell lysates from human dermal fibroblasts neonatal (treated with II1beta)

    Predicted band size: 66 kDa
    Observed band size: 66 kDa

  • Anti-MMP14 antibody - Hinge region (ab38971) at 1/1000 dilution + HT1080 whole cell lysate at 30 µg

    Secondary
    HRP-conjugated donkey anti-rabbit

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 64 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

  • All lanes : Anti-MMP14 antibody - Hinge region (ab38971) at 1/5000 dilution

    Lane 1 : Whole cell lysate prepared from HT1080 cells
    Lane 2 : Whole cell lysate prepared from human vascular smooth muscle cells

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : HRP conjugated donkey anti-rabbit IgG at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 63,65 kDa why is the actual band size different from the predicted?
    Additional bands at: 20 kDa (possible non-specific binding)


    Exposure time: 30 seconds


    Blocked using 5% milk for 1 hour at 20°C.

    See Abreview

  • ab38971 at 10ug/ml staining human HT1080 cells by ICC/IF. The cells were paraformaldhyde fixed and incubated with the antibody for 1 hour. A FITC conjugated donkey anti-rabbit antibody was used as the secondary.

    See Abreview

  • Ab38971 staining Human normal myometrium tissue. Staining is localized to nuclear and cytoplasmic compartment.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 (ab64214) in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification ma

References

This product has been referenced in:
  • Almalki SG  et al. MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. Stem Cells Transl Med 6:1385-1398 (2017). WB ; Pig . Read more (PubMed: 28213979) »
  • Dole NS  et al. Osteocyte-Intrinsic TGF-ß Signaling Regulates Bone Quality through Perilacunar/Canalicular Remodeling. Cell Rep 21:2585-2596 (2017). IHC-P ; Mouse . Read more (PubMed: 29186693) »
See all 28 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-4 of 4 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Bone-Osteocytes)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Bone-Osteocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 22 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080 and human vascular smooth muscle cells)
Loading amount
5 µg
Specification
HT1080 and human vascular smooth muscle cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 14 2010

Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080)
Loading amount
30 µg
Specification
HT1080
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 14 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (fibrosarcoma)
Specification
fibrosarcoma
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 02 2007

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