Key features and details
- Mouse monoclonal [LEM-2/63.1] to MMP14
- Suitable for: WB, IP, ELISA, IHC-Fr, Flow Cyt, Inhibition Assay, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2a
Product nameAnti-MMP14 antibody [LEM-2/63.1]
See all MMP14 primary antibodies
DescriptionMouse monoclonal [LEM-2/63.1] to MMP14
Tested applicationsSuitable for: WB, IP, ELISA, IHC-Fr, Flow Cyt, Inhibition Assay, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis, Macaque monkey
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.0536% PBS
Concentration information loading...
PurityTissue culture supernatant
Purification notesPurified from TCS.
Light chain typekappa
Our Abpromise guarantee covers the use of ab78738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 66 kDa.|
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|Inhibition Assay||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionSeems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
Tissue specificityExpressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsThe precursor is cleaved by a furin endopeptidase.
Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Matrix metallopeptidase 14 (membrane inserted) antibody
- Matrix metalloproteinase 14 antibody
- Matrix metalloproteinase-14 antibody
ab78738 has been referenced in 15 publications.
- Röhl J et al. Invasion by activated macrophages requires delivery of nascent membrane-type-1 matrix metalloproteinase through late endosomes/lysosomes to the cell surface. Traffic 20:661-673 (2019). PubMed: 31297933
- Hwang HJ et al. Multiplex quantitative analysis of stroma-mediated cancer cell invasion, matrix remodeling, and drug response in a 3D co-culture model of pancreatic tumor spheroids and stellate cells. J Exp Clin Cancer Res 38:258 (2019). PubMed: 31200779
- Tikhonova AN et al. The bone marrow microenvironment at single-cell resolution. Nature 569:222-228 (2019). PubMed: 30971824
- Kumar S et al. MMP Secretion Rate and Inter-invadopodia Spacing Collectively Govern Cancer Invasiveness. Biophys J 114:650-662 (2018). PubMed: 29414711
- Arkadash V et al. Development of High Affinity and High Specificity Inhibitors of Matrix Metalloproteinase 14 through Computational Design and Directed Evolution. J Biol Chem 292:3481-3495 (2017). PubMed: 28087697