Key features and details
- Rabbit polyclonal to MMP16
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab53145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 57 kDa.|
FunctionEndopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.
Tissue specificityExpressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Developmental stageExpressed in tissues undergoing reconstruction. Present in fetal tissues, especially in brain. Expression seems to decline with advanced development.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsThe precursor is cleaved by a furin endopeptidase.
Cellular localizationCell membrane. Localized at the cell surface of melanoma cells and Secreted > extracellular space > extracellular matrix. Cell surface. Localized at the cell surface of melanoma cells.
- Information by UniProt
- C8orf57 antibody
- DKFZp761D112 antibody
- Matrix metalloproteinase 16 antibody
ab53145 has been referenced in 2 publications.
- Wang H et al. Cryptotanshinone inhibits lung cancer invasion via microRNA-133a/matrix metalloproteinase 14 regulation. Oncol Lett 18:2554-2559 (2019). PubMed: 31452742
- Hadchouel A et al. Matrix metalloproteinase gene polymorphisms and bronchopulmonary dysplasia: identification of MMP16 as a new player in lung development. PLoS ONE 3:e3188 (2008). WB ; Human . PubMed: 18784838