Anti-MMP17 antibody [EP1270Y] (ab51075)


  • Product name
    Anti-MMP17 antibody [EP1270Y]
  • Description
    Rabbit monoclonal [EP1270Y] to MMP17
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IHC-FoFrmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MMP17 aa 150-250 (internal sequence). The exact sequence is proprietary.

  • Positive control
    • Mouse brain lysate, human lymphoma tissue or HeLa cells.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab51075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 67 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22022509
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin.
    • Tissue specificity
      Expressed in brain, leukocytes, colon, ovary testis and breast cancer. Expressed also in many transformed and non-transformed cell types.
    • Sequence similarities
      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • Domain
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      The precursor is cleaved by a furin endopeptidase.
    • Cellular localization
      Cell membrane. Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Matrix metalloproteinase 17 antibody
      • Matrix metalloproteinase 17 membrane inserted antibody
      • Matrix metalloproteinase-17 antibody
      • Membrane type 4 matrix metalloproteinase antibody
      • Membrane type matrix metalloproteinase 4 antibody
      • Membrane-type matrix metalloproteinase 4 antibody
      • Membrane-type-4 matrix metalloproteinase antibody
      • MMP 17 antibody
      • MMP-17 antibody
      • Mmp17 antibody
      • MMP17_HUMAN antibody
      • MT MMP 4 antibody
      • MT MMP4 antibody
      • MT-MMP 4 antibody
      • MT4 MMP antibody
      • MT4-MMP antibody
      • MT4MMP antibody
      • MTMMP 4 antibody
      • MTMMP4 antibody
      see all


    • Anti-MMP17 antibody [EP1270Y] (ab51075) at 1/1000 dilution + Mouse brain lysate at 10 µg

      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 67 kDa
      Observed band size: 58 kDa (why is the actual band size different from the predicted?)

    • Ab51075 (1:50) staining human MMP17 in human lymphoma tissue by immunohistochemistry using paraffin embedded tissue.
    • Immunofluorescent staining of HeLa cells using ab51075 (1:50).
    • Immunohistochemical analysis of rat basolateral amygdala tissue, staining MMP17 with ab51075.

      Tissue was permeabilized with 0.5% Triton-X 100 before incubating with primary antibody (1/100) for 48 hours at 4°C. An AlexaFluor®568 goat anti-rabbit IgG was used as the secondary antibody.


    This product has been referenced in:
    • Clemente C  et al. MT4-MMP deficiency increases patrolling monocyte recruitment to early lesions and accelerates atherosclerosis. Nat Commun 9:910 (2018). Read more (PubMed: 29500407) »
    • Blanco MJ  et al. Developmental expression of membrane type 4-matrix metalloproteinase (Mt4-mmp/Mmp17) in the mouse embryo. PLoS One 12:e0184767 (2017). Read more (PubMed: 28926609) »

    See all 8 Publications for this product

    Customer reviews and Q&As

    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Human Cell lysate - whole cell (human cell lines)
    human cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Mrs. Monika Schumacher

    Verified customer

    Submitted Feb 25 2015

    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (10 %)
    Guinea pig Tissue lysate - whole (Liver)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Mrs. Monika Schumacher

    Verified customer

    Submitted Jan 23 2015

    r these details. I am sorry this vial of antibody has not worked for this customer.

    As requested, we are pleased to arrange an alternative free of charge replacement of ab39028. I can confirm that 1 vial of ab39028 has been added to your ord...

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    Thank you for your message and for providing this further information.

    I am sorry there are no further suggestions to help improve the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased t...

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    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will provide us with vital informa...

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    Thank you for your inquiry.

    The concentration of ab51075 is lot dependent. The lot that we have in stock at the moment and of the last vial you receivedhas a concentration of 0.224 mg/mL.

    I also would like to comment on the seemingl...

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    Vielen Dank für Ihren Anruf gestern.

    Ich habe jetzt genauere Information zum Immunogen erhalten:

    Es liegt zwischen den Aminosäuren AA190 und 230 (SwissProt ID Q9ULZ9).

    Ich hoffe, diese Information hilft Ihnen her...

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    Thank you for your enquiry. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instr...

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    Thank you for your inquiry. I am happy to confirm that the concentration of lot GR19885 is 0.224 mg/mL. I also would like to comment on the seemingly low concentration: The IgG produced by rabbits have greater affinity and greater sensitivity ...

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