Anti-MMP19 antibody (ab53146)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-MMP19 antibody
See all MMP19 primary antibodies -
DescriptionRabbit polyclonal to MMP19
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Host speciesRabbit
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Specificityab53146 detects endogenous levels of total MMP19 protein.
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Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
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Species reactivityReacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide derived from human MMP19.
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Positive control
- WB: Extracts of HuvEc cells IHC-P: Human breast carcinoma tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab53146 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab53146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 57 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | 1/10000. |
Target
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FunctionEndopeptidase that degrades various components of the extracellular matrix, such as aggrecan and cartilage oligomeric matrix protein (comp), during development, haemostasis and pathological conditions (arthritic disease). May also play a role in neovascularization or angiogenesis. Hydrolyzes collagen type IV, laminin, nidogen, nascin-C isoform, fibronectin, and type I gelatin.
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Tissue specificityExpressed in mammary gland, placenta, lung, pancreas, ovary, small intestine, spleen, thymus, prostate, testis colon, heart and blood vessel walls. Not detected in brain and peripheral blood leukocytes. Also expressed in the synovial fluid of normal and rheumatoid patients.
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Involvement in diseaseMay play a role in pathological processes participating in rheumatoid arthritis (RA)-associated joint tissue destruction. Autoantigen anti-MMP19 are frequent in RA patients.
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Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
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Post-translational
modificationsActivated by autolytic cleavage after Lys-97. -
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
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Database links
- Entrez Gene: 4327 Human
- Entrez Gene: 304608 Rat
- Omim: 601807 Human
- SwissProt: Q99542 Human
- Unigene: 591033 Human
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Alternative names
- Matrix metalloproteinase 18 antibody
- Matrix metalloproteinase 19 antibody
- Matrix metalloproteinase RASI antibody
see all
Images
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Western blot - Anti-MMP19 antibody (ab53146)All lanes : Anti-MMP19 antibody (ab53146) at 1/500 dilution
Lane 1 : Extracts of Huv Ec cells with no peptide
Lane 2 : Extracts of Huv Ec cells with peptide
Predicted band size: 57 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP19 antibody (ab53146)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53146 at a dilution of 1/50-1/100. Right hand image: treated with immunising peptide. Left hand image: untreated.
Datasheets and documents
References
This product has been referenced in:
- Liu RY et al. Overexpression of asparagine synthetase and matrix metalloproteinase 19 confers cisplatin sensitivity in nasopharyngeal carcinoma cells. Mol Cancer Ther 12:2157-66 (2013). Human . Read more (PubMed: 23956056) »
- Wang L et al. Co-expression of MMP-14 and MMP-19 predicts poor survival in human glioma. Clin Transl Oncol 15:139-45 (2013). Read more (PubMed: 22855183) »
