Overview

  • Product name
    Anti-MMP19 antibody
    See all MMP19 primary antibodies
  • Description
    Rabbit polyclonal to MMP19
  • Host species
    Rabbit
  • Specificity
    ab53146 detects endogenous levels of total MMP19 protein.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from human MMP19.

  • Positive control
    • WB: Extracts of HuvEc cells IHC-P: Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab53146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 57 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

Target

  • Function
    Endopeptidase that degrades various components of the extracellular matrix, such as aggrecan and cartilage oligomeric matrix protein (comp), during development, haemostasis and pathological conditions (arthritic disease). May also play a role in neovascularization or angiogenesis. Hydrolyzes collagen type IV, laminin, nidogen, nascin-C isoform, fibronectin, and type I gelatin.
  • Tissue specificity
    Expressed in mammary gland, placenta, lung, pancreas, ovary, small intestine, spleen, thymus, prostate, testis colon, heart and blood vessel walls. Not detected in brain and peripheral blood leukocytes. Also expressed in the synovial fluid of normal and rheumatoid patients.
  • Involvement in disease
    May play a role in pathological processes participating in rheumatoid arthritis (RA)-associated joint tissue destruction. Autoantigen anti-MMP19 are frequent in RA patients.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    Activated by autolytic cleavage after Lys-97.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
    • Alternative names
      • Matrix metalloproteinase 18 antibody
      • Matrix metalloproteinase 19 antibody
      • Matrix metalloproteinase RASI antibody
      • Matrix metalloproteinase-18 antibody
      • Matrix metalloproteinase-19 antibody
      • MMP 18 antibody
      • MMP 19 antibody
      • MMP-18 antibody
      • MMP-19 antibody
      • MMP18 antibody
      • MMP19 antibody
      • MMP19_HUMAN antibody
      • RASI 1 antibody
      • RASI antibody
      • RASI1 antibody
      see all

    Images

    • Western blot - Anti-MMP19 antibody (ab53146)
      Western blot - Anti-MMP19 antibody (ab53146)
      All lanes : Anti-MMP19 antibody (ab53146) at 1/500 dilution

      Lane 1 : Extracts of Huv Ec cells with no peptide
      Lane 2 : Extracts of Huv Ec cells with peptide

      Predicted band size: 57 kDa
      Observed band size: 62 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP19 antibody (ab53146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP19 antibody (ab53146)
      Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53146 at a dilution of 1/50-1/100. Right hand image: treated with immunising peptide. Left hand image: untreated.

    References

    This product has been referenced in:
    • Liu RY  et al. Overexpression of asparagine synthetase and matrix metalloproteinase 19 confers cisplatin sensitivity in nasopharyngeal carcinoma cells. Mol Cancer Ther 12:2157-66 (2013). Human . Read more (PubMed: 23956056) »
    • Wang L  et al. Co-expression of MMP-14 and MMP-19 predicts poor survival in human glioma. Clin Transl Oncol 15:139-45 (2013). Read more (PubMed: 22855183) »

    See all 3 Publications for this product

    Publishing research using ab53146? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab53146.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up