Anti-MMP19 antibody (ab53146)
Key features and details
- Rabbit polyclonal to MMP19
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MMP19 antibody
See all MMP19 primary antibodies -
Description
Rabbit polyclonal to MMP19 -
Host species
Rabbit -
Specificity
ab53146 detects endogenous levels of total MMP19 protein. -
Tested applications
Suitable for: WB, IHC-P, ELISAmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide derived from human MMP19.
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Positive control
- WB: Extracts of HuvEc cells IHC-P: Human breast carcinoma tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab53146 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab53146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 57 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | 1/10000. |
Target
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Function
Endopeptidase that degrades various components of the extracellular matrix, such as aggrecan and cartilage oligomeric matrix protein (comp), during development, haemostasis and pathological conditions (arthritic disease). May also play a role in neovascularization or angiogenesis. Hydrolyzes collagen type IV, laminin, nidogen, nascin-C isoform, fibronectin, and type I gelatin. -
Tissue specificity
Expressed in mammary gland, placenta, lung, pancreas, ovary, small intestine, spleen, thymus, prostate, testis colon, heart and blood vessel walls. Not detected in brain and peripheral blood leukocytes. Also expressed in the synovial fluid of normal and rheumatoid patients. -
Involvement in disease
May play a role in pathological processes participating in rheumatoid arthritis (RA)-associated joint tissue destruction. Autoantigen anti-MMP19 are frequent in RA patients. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsActivated by autolytic cleavage after Lys-97. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4327 Human
- Entrez Gene: 304608 Rat
- Omim: 601807 Human
- SwissProt: Q99542 Human
- Unigene: 591033 Human
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Alternative names
- Matrix metalloproteinase 18 antibody
- Matrix metalloproteinase 19 antibody
- Matrix metalloproteinase RASI antibody
see all
Images
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Western blot - Anti-MMP19 antibody (ab53146)All lanes : Anti-MMP19 antibody (ab53146) at 1/500 dilution
Lane 1 : Extracts of Huv Ec cells with no peptide
Lane 2 : Extracts of Huv Ec cells with peptide
Predicted band size: 57 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP19 antibody (ab53146)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53146 at a dilution of 1/50-1/100. Right hand image: treated with immunising peptide. Left hand image: untreated.
Protocols
Datasheets and documents
References (4)
ab53146 has been referenced in 4 publications.
- Tian G et al. MicroRNA-16 inhibits the proliferation, migration and invasion of non-small cell lung carcinoma cells by down-regulating matrix metalloproteinase-19 expression. Eur Rev Med Pharmacol Sci 23:5260-5269 (2019). PubMed: 31298377
- Liu RY et al. Overexpression of asparagine synthetase and matrix metalloproteinase 19 confers cisplatin sensitivity in nasopharyngeal carcinoma cells. Mol Cancer Ther 12:2157-66 (2013). Human . PubMed: 23956056
- Wang L et al. Co-expression of MMP-14 and MMP-19 predicts poor survival in human glioma. Clin Transl Oncol 15:139-45 (2013). PubMed: 22855183
- Jackson V et al. Matrix metalloproteinase 14 and 19 expression is associated with thoracic aortic aneurysms. J Thorac Cardiovasc Surg 144:459-66 (2012). Human . PubMed: 21955474
