• Product name
  • Description
    Rabbit polyclonal to MMP19
  • Host species
  • Specificity
    ab53146 detects endogenous levels of total MMP19 protein.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from human MMP19.

  • Positive control
    • WB: Extracts of HuvEc cells IHC-P: Human breast carcinoma tissue



Our Abpromise guarantee covers the use of ab53146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 57 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.


  • Function
    Endopeptidase that degrades various components of the extracellular matrix, such as aggrecan and cartilage oligomeric matrix protein (comp), during development, haemostasis and pathological conditions (arthritic disease). May also play a role in neovascularization or angiogenesis. Hydrolyzes collagen type IV, laminin, nidogen, nascin-C isoform, fibronectin, and type I gelatin.
  • Tissue specificity
    Expressed in mammary gland, placenta, lung, pancreas, ovary, small intestine, spleen, thymus, prostate, testis colon, heart and blood vessel walls. Not detected in brain and peripheral blood leukocytes. Also expressed in the synovial fluid of normal and rheumatoid patients.
  • Involvement in disease
    May play a role in pathological processes participating in rheumatoid arthritis (RA)-associated joint tissue destruction. Autoantigen anti-MMP19 are frequent in RA patients.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    Activated by autolytic cleavage after Lys-97.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Matrix metalloproteinase 18 antibody
    • Matrix metalloproteinase 19 antibody
    • Matrix metalloproteinase RASI antibody
    • Matrix metalloproteinase-18 antibody
    • Matrix metalloproteinase-19 antibody
    • MMP 18 antibody
    • MMP 19 antibody
    • MMP-18 antibody
    • MMP-19 antibody
    • MMP18 antibody
    • MMP19 antibody
    • MMP19_HUMAN antibody
    • RASI 1 antibody
    • RASI antibody
    • RASI1 antibody
    see all


  • All lanes : Anti-MMP19 antibody (ab53146) at 1/500 dilution

    Lane 1 : Extracts of Huv Ec cells with no peptide
    Lane 2 : Extracts of Huv Ec cells with peptide

    Predicted band size: 57 kDa
    Observed band size: 62 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53146 at a dilution of 1/50-1/100. Right hand image: treated with immunising peptide. Left hand image: untreated.


This product has been referenced in:
  • Liu RY  et al. Overexpression of asparagine synthetase and matrix metalloproteinase 19 confers cisplatin sensitivity in nasopharyngeal carcinoma cells. Mol Cancer Ther 12:2157-66 (2013). Human . Read more (PubMed: 23956056) »
  • Wang L  et al. Co-expression of MMP-14 and MMP-19 predicts poor survival in human glioma. Clin Transl Oncol 15:139-45 (2013). Read more (PubMed: 22855183) »
See all 3 Publications for this product

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