Overview

  • Product name
  • Description
    Rabbit polyclonal to MMP19 rasi6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 214-489 of Human MMP19 rasi6 (UniProt ID: Q99542).

  • Positive control
    • H1299, HCT116 and MCF7 cell lysates; Human hepatoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function
    Endopeptidase that degrades various components of the extracellular matrix, such as aggrecan and cartilage oligomeric matrix protein (comp), during development, haemostasis and pathological conditions (arthritic disease). May also play a role in neovascularization or angiogenesis. Hydrolyzes collagen type IV, laminin, nidogen, nascin-C isoform, fibronectin, and type I gelatin.
  • Tissue specificity
    Expressed in mammary gland, placenta, lung, pancreas, ovary, small intestine, spleen, thymus, prostate, testis colon, heart and blood vessel walls. Not detected in brain and peripheral blood leukocytes. Also expressed in the synovial fluid of normal and rheumatoid patients.
  • Involvement in disease
    Note=May play a role in pathological processes participating in rheumatoid arthritis (RA)-associated joint tissue destruction. Autoantigen anti-MMP19 are frequent in RA patients.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    Activated by autolytic cleavage after Lys-97.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Matrix metalloproteinase 18 antibody
    • Matrix metalloproteinase 19 isoform rasi1 antibody
    • Matrix metalloproteinase 19 isoform rasi9 antibody
    • Matrix metalloproteinase RASI antibody
    • Matrix metalloproteinase-18 antibody
    • Matrix metalloproteinase-19 antibody
    • MMP-18 antibody
    • MMP-19 antibody
    • MMP18 antibody
    • MMP19 antibody
    • MMP19_HUMAN antibody
    • Preproprotein matrix metalloproteinase 19 isoform rasi6 antibody
    • RASI 1 antibody
    • RASI1 antibody
    see all

Images

  • All lanes : Anti-MMP19 rasi6 antibody (ab154496) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HCT116 whole cell lysate
    Lane 3 : MCF7 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 57 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human hepatoma tissue, labeling MMP19 rasi6 using ab154496 at a 1/500 dilution.

References

ab154496 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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