Key features and details
- Rabbit polyclonal to MMP19 rasi6
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MMP19 rasi6 antibody
DescriptionRabbit polyclonal to MMP19 rasi6
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant protein fragment, corresponding to a region within amino acids 214-489 of Human MMP19 rasi6 (UniProt ID: Q99542).
- H1299, HCT116 and MCF7 cell lysates; Human hepatoma tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 57 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionEndopeptidase that degrades various components of the extracellular matrix, such as aggrecan and cartilage oligomeric matrix protein (comp), during development, haemostasis and pathological conditions (arthritic disease). May also play a role in neovascularization or angiogenesis. Hydrolyzes collagen type IV, laminin, nidogen, nascin-C isoform, fibronectin, and type I gelatin.
Tissue specificityExpressed in mammary gland, placenta, lung, pancreas, ovary, small intestine, spleen, thymus, prostate, testis colon, heart and blood vessel walls. Not detected in brain and peripheral blood leukocytes. Also expressed in the synovial fluid of normal and rheumatoid patients.
Involvement in diseaseNote=May play a role in pathological processes participating in rheumatoid arthritis (RA)-associated joint tissue destruction. Autoantigen anti-MMP19 are frequent in RA patients.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsActivated by autolytic cleavage after Lys-97.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Matrix metalloproteinase 18 antibody
- Matrix metalloproteinase 19 isoform rasi1 antibody
- Matrix metalloproteinase 19 isoform rasi9 antibody
All lanes : Anti-MMP19 rasi6 antibody (ab154496) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HCT116 whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa
7.5% SDS PAGE
Immunohistochemical analysis of formalin fixed, paraffin embedded Human hepatoma tissue, labeling MMP19 rasi6 using ab154496 at a 1/500 dilution.
ab154496 has not yet been referenced specifically in any publications.