Anti-MMP2 antibody [4D3] (ab2462)
- Datasheet
- References (18)
- Protocols
Overview
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Product nameAnti-MMP2 antibody [4D3]
See all MMP2 primary antibodies -
DescriptionMouse monoclonal [4D3] to MMP2
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Host speciesMouse
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SpecificityBinds to human MMP-2 and pro-MMP2. The antibody was tested for cross-reactivity with MMP-1, MMP-3, and MMP-9 and showed mild cross-reactivity with MMP-3.
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Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
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Species reactivityReacts with: Rabbit, Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human MMP2 aa 557-569 (internal sequence).
Sequence:TSLGLPPDVQRVD
Database link: P08253 -
Positive control
- Colon carcinoma. Placental tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone number4D3
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IsotypeIgG1
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Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab2462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 19910495 | |
| WB | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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FunctionUbiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. -
Tissue specificityProduced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
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Involvement in diseaseDefects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
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Sequence similaritiesBelongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains. -
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
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Post-translational
modificationsPhosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3. -
Cellular localizationSecreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
- Information by UniProt
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Database links
- Entrez Gene: 4313 Human
- Entrez Gene: 17390 Mouse
- Entrez Gene: 100009000 Rabbit
- Entrez Gene: 81686 Rat
- Omim: 120360 Human
- SwissProt: P08253 Human
- SwissProt: P33434 Mouse
- SwissProt: P50757 Rabbit
see all -
Alternative names
- 72 kDa gelatinase antibody
- 72kD type IV collagenase antibody
- CLG 4 antibody
see all
Images
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![Western blot - Anti-MMP2 antibody [4D3] (ab2462)](https://www.abcam.com/ps/products/2/ab2462/Images/ab2462-220493-ab2462.jpg)
Western blot - Anti-MMP2 antibody [4D3] (ab2462)400 ng samples of recombinant human pro-enzyme (lane 1 = MMP-1; lane 2 = MMP-3; lane 3 = MMP-2; lane 4 = MMP-9) were subjected to SDS-PAGE, transferred to PVDF membrane, and detected by immunoblotting with ab2462 specific for MMP-2.
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![Western blot - Anti-MMP2 antibody [4D3] (ab2462)](https://www.abcam.com/ps/products/2/ab2462/Images/ab2462-197048-ab2462wb.jpg)
Western blot - Anti-MMP2 antibody [4D3] (ab2462)This image is courtesy of an anonymous AbreviewAnti-MMP2 antibody [4D3] (ab2462) at 1/5000 dilution + Rabbit aorta tissue lysate at 40 µg
Secondary
HRP-conjugate Sheep anti-mouse IgG polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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![Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody [4D3] (ab2462)](https://www.abcam.com/ps/products/2/ab2462/Images/MMP2-Primary-antibodies-ab2462-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody [4D3] (ab2462)Image from Kean MJ et al, J Cell Sci. 2009 Nov 15;122(Pt 22):4089-98, Fig 1. DOI 10.1242/?jcs.052761ab2462 staining MMP2 in HT-1080 cells by Immunocytochemistry/ Immunofluorescence.
Cells were grown on glass coverslips, treated with PMA and then fixed with 4% (w/v) paraformaldehyde in phosphate buffered saline (PBS). Samples were then permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% (w/v) skimmed milk powder in PBS before staining with ab2462 and the appropriate secondary antibody, followed by washing and mounting.
Scale bar: 10 µm.
Protocols
References
This product has been referenced in:
- Liang X et al. Reactive oxygen species mediated oxidative stress links diabetes and atrial fibrillation. Mol Med Rep 17:4933-4940 (2018). Read more (PubMed: 29393403) »
- Cao Q et al. Dickkopf-3 upregulation mediates the cardioprotective effects of curcumin on chronic heart failure. Mol Med Rep 17:7249-7257 (2018). Read more (PubMed: 29568962) »
