• Product name
    Anti-MMP2 antibody [4D3]
    See all MMP2 primary antibodies
  • Description
    Mouse monoclonal [4D3] to MMP2
  • Host species
  • Specificity
    Binds to human MMP-2 and pro-MMP2. The antibody was tested for cross-reactivity with MMP-1, MMP-3, and MMP-9 and showed mild cross-reactivity with MMP-3.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rabbit, Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human MMP2 aa 557-569 (internal sequence).


    Database link: P08253

  • Positive control
    • Colon carcinoma. Placental tissue



Our Abpromise guarantee covers the use of ab2462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19910495
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.


  • Function
    Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
    -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
    PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
  • Tissue specificity
    Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
  • Involvement in disease
    Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
    The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 72 kDa gelatinase antibody
    • 72kD type IV collagenase antibody
    • CLG 4 antibody
    • CLG 4A antibody
    • CLG4 antibody
    • CLG4A antibody
    • Collagenase Type 4 alpha antibody
    • Collagenase type IV A antibody
    • Gelatinase A antibody
    • Gelatinase alpha antibody
    • Gelatinase neutrophil antibody
    • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
    • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 2 antibody
    • Matrix metalloproteinase II antibody
    • Matrix metalloproteinase-2 antibody
    • MMP 2 antibody
    • MMP II antibody
    • MMP-2 antibody
    • MMP2 antibody
    • MMP2_HUMAN antibody
    • MONA antibody
    • Neutrophil gelatinase antibody
    • PEX antibody
    • TBE 1 antibody
    • TBE-1 antibody
    see all


  • 400 ng samples of recombinant human pro-enzyme (lane 1 = MMP-1; lane 2 = MMP-3; lane 3 = MMP-2; lane 4 = MMP-9) were subjected to SDS-PAGE, transferred to PVDF membrane, and detected by immunoblotting with ab2462 specific for MMP-2.

  • Anti-MMP2 antibody [4D3] (ab2462) at 1/5000 dilution + Rabbit aorta tissue lysate at 40 µg

    HRP-conjugate Sheep anti-mouse IgG polyclonal

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 72 kDa
    why is the actual band size different from the predicted?

    Exposure time: 30 seconds

    See Abreview

  • ab2462 staining MMP2 in HT-1080 cells by Immunocytochemistry/ Immunofluorescence.
    Cells were grown on glass coverslips, treated with PMA and then fixed with 4% (w/v) paraformaldehyde in phosphate buffered saline (PBS). Samples were then permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% (w/v) skimmed milk powder in PBS before staining with ab2462 and the appropriate secondary antibody, followed by washing and mounting.

    Scale bar: 10 µm.


This product has been referenced in:
  • Liang X  et al. Reactive oxygen species mediated oxidative stress links diabetes and atrial fibrillation. Mol Med Rep 17:4933-4940 (2018). Read more (PubMed: 29393403) »
  • Cao Q  et al. Dickkopf-3 upregulation mediates the cardioprotective effects of curcumin on chronic heart failure. Mol Med Rep 17:7249-7257 (2018). Read more (PubMed: 29568962) »
See all 18 Publications for this product

Customer reviews and Q&As

1-10 of 14 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Rabbit Tissue sections (skin)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: proteinase K
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Aug 11 2016

Immunohistochemistry (Frozen sections)
Human Tissue sections (skin)
Blocking step
human serum in 0.25% casein as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 16 2016

Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Rabbit Tissue lysate - whole (aorta)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 24 2013


Thank you for confirming the quality of the secondary antibody. I can believe that the issue is with the MMP2 antibody but I am surprised that all three antibodies are giving you trouble.

Are the problems other people from other labs reporting about the same MMP antibodies from Abcam? Or just the MMP2 antibody?

If you are interested in trying a different MMP2 antibody, I will be happy to send a free replacement if your order is within the limits of our guarantee. Do you know when the order was placed, and the distributor

Read More


Thank you for the protocol details.

Do the blots for MMP1 and MMP9 look similar to the MMP2 blot? Can you please tell me the species of KB cells?

I am a little concerned aboutthe quality of the sample, given the multiple bands between 50 and 75, and the smearing at the top of the wells. What is the protein that you are staining successfully? Are you using the same secondary for blots of that protein? Is there any possibility that the secondary antibody is the issue?

I look forward to your reply.

Read More


Thank you for bringing this to our attention. Can you please describe your samples, how you prepare them for loading into the gel, how much antibody you use and how long you incubate, and any other details you think may be relevant? Are you confident the secondary antibody is effective and is not the source of the background? Have you stained blots of these samples with any other antibodies, and, if so, were the results as you expected?

Read More


Thank you for your enquiry.

Thank you for contacting us and sorry for the delay in getting back to you.

As discussed over the phone, unfortunately I can now confirm that the concentration of the Anti-MMP2 antibody [4D3] (ab2462) has not as yet been determined.

The concentration of unpurified antibodies, such as this one sold as tissue culture supernatant, will not have a concentration determined. Antibody concentration is usually determined by protein assay, and tissue culture supernatant will contain a lot of other proteins other than IgG, which means the antibody quantification would not be accurate.

I can however confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml.

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab2462.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

Read More


Thank you for your enquiry. We suggest a concentration of 1:20, however we also recommend a pilot experiment using a serial dilution with a range from 1:5 to 1:300 to determine the optimal concentration for the tissue you are staining. I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

Read More


Thank you for your enquiry. This Ab reacts with both the pro- and cleaved form of MMP2. Placental tissue can be used as a positive control. Should you have any further questions then please do not hesitate to get back in touch with me.

Read More

1-10 of 14 Abreviews or Q&A

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