Key features and details
- Mouse monoclonal [4D3] to MMP2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Product nameAnti-MMP2 antibody [4D3]
See all MMP2 primary antibodies
DescriptionMouse monoclonal [4D3] to MMP2
SpecificityBinds to human MMP-2 and pro-MMP2. The antibody was tested for cross-reactivity with MMP-1, MMP-3, and MMP-9 and showed mild cross-reactivity with MMP-3.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Colon carcinoma. Placental tissue
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
PurityTissue culture supernatant
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
FunctionUbiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
Tissue specificityProduced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
Involvement in diseaseDefects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsPhosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
Cellular localizationSecreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
- Information by UniProt
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400 ng samples of recombinant human pro-enzyme (lane 1 = MMP-1; lane 2 = MMP-3; lane 3 = MMP-2; lane 4 = MMP-9) were subjected to SDS-PAGE, transferred to PVDF membrane, and detected by immunoblotting with ab2462 specific for MMP-2.
Anti-MMP2 antibody [4D3] (ab2462) at 1/5000 dilution + Rabbit aorta tissue lysate at 40 µg
HRP-conjugate Sheep anti-mouse IgG polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
ab2462 has been referenced in 30 publications.
- Wei D et al. Circular RNA circ_0000043 promotes endometrial carcinoma progression by regulating miR-1271-5p/CTNND1 axis. Arch Gynecol Obstet 303:1075-1087 (2021). PubMed: 33128584
- Zhang C et al. Ethanol Extracts of Solanum lyratum Thunb Regulate Ovarian Cancer Cell Proliferation, Apoptosis, and Epithelial-to-Mesenchymal Transition (EMT) via the ROS-Mediated p53 Pathway. J Immunol Res 2021:5569354 (2021). PubMed: 33869638
- Gong R et al. A Comparison of Subconjunctival Wound Healing between Different Methods of Dissecting Subconjunctival Tissues. Ophthalmic Res 64:99-107 (2021). PubMed: 32564013
- Pan X et al. Genetic and Clinical Characterization of HOXB2 in Glioma. Onco Targets Ther 13:10465-10473 (2020). PubMed: 33116626
- Xie H et al. ROS/NF-?B Signaling Pathway-Mediated Transcriptional Activation of TRIM37 Promotes HBV-Associated Hepatic Fibrosis. Mol Ther Nucleic Acids 22:114-123 (2020). PubMed: 32916597