Application
Western blot
Sample
Human Cell lysate - whole cell (Fibroblasts)
Loading amount
60000 cells
Specification
Fibroblasts
Gel Running Conditions
Reduced Denaturing (10% acrylamide)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 3% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 74 kDa
Bruno da Rocha-Azevedo
Verified customer
Submitted Oct 30 2012