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    mmp2-antibody-ab227755.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP
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Anti-MMP2 antibody (ab227755)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab227755)

    Key features and details

    • Rabbit polyclonal to MMP2
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-MMP2 antibody
      See all MMP2 primary antibodies
    • Description

      Rabbit polyclonal to MMP2
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig
    • Immunogen

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • Positive control

      • IHC-P: Human placenta tissue.
    • General notes

      This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.60
      Preservative: 0.1% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Adhesion / ECM
      • Matrix Metalloproteinases
      • MMP
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • MMPs
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Metalloprotease
      • MMPs
      • Cancer
      • Tumor biomarkers
      • Enzymes
      • MMPs
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Neuroscience
      • Diseases
      • Neuroscience
      • Processes

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human MMP2 protein (Active) (ab81550)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab227755 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Primary antibody incubation for 30 minutes at room temperature.

    Notes
    IHC-P
    1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Primary antibody incubation for 30 minutes at room temperature.

    Target

    • Function

      Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
      -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
      PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
    • Tissue specificity

      Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
    • Involvement in disease

      Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
    • Sequence similarities

      Belongs to the peptidase M10A family.
      Contains 3 fibronectin type-II domains.
      Contains 4 hemopexin-like domains.
    • Domain

      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications

      Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
      The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
    • Target information above from: UniProt accession P08253 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 386583386583 Chicken
      • Entrez Gene: 282872 Cow
      • Entrez Gene: 403733 Dog
      • Entrez Gene: 4313 Human
      • Entrez Gene: 17390 Mouse
      • Entrez Gene: 397391 Pig
      • Entrez Gene: 100009000 Rabbit
      • Entrez Gene: 81686 Rat
      • Omim: 120360 Human
      • SwissProt: Q90611 Chicken
      • SwissProt: Q9GLE5 Cow
      • SwissProt: P08253 Human
      • SwissProt: P33434 Mouse
      • SwissProt: P50757 Rabbit
      • SwissProt: P33436 Rat
      • Unigene: 513617 Human
      • Unigene: 29564 Mouse
      • Unigene: 6422 Rat
      see all
    • Alternative names

      • 72 kDa gelatinase antibody
      • 72kD type IV collagenase antibody
      • CLG 4 antibody
      • CLG 4A antibody
      • CLG4 antibody
      • CLG4A antibody
      • Collagenase Type 4 alpha antibody
      • Collagenase type IV A antibody
      • Gelatinase A antibody
      • Gelatinase alpha antibody
      • Gelatinase neutrophil antibody
      • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
      • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody
      • Matrix Metalloproteinase 2 antibody
      • Matrix metalloproteinase II antibody
      • Matrix metalloproteinase-2 antibody
      • MMP 2 antibody
      • MMP II antibody
      • MMP-2 antibody
      • MMP2 antibody
      • MMP2_HUMAN antibody
      • MONA antibody
      • Neutrophil gelatinase antibody
      • PEX antibody
      • TBE 1 antibody
      • TBE-1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab227755)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab227755)

      Formalin-fixed, paraffin-embedded human placenta tissue stained for MMP2 using ab227755 at 1/100 dilution in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab227755? Please let us know so that we can cite the reference in this datasheet.

    ab227755 has been referenced in 2 publications.

    • Liu X  et al. CREB1 acts via the miR-922/ARID2 axis to enhance malignant behavior of liver cancer cells. Oncol Rep 45:N/A (2021). PubMed: 33786634
    • Koldysheva EV  et al. Role of Matrix Metalloproteinase-2 in the Development of Cyclophosphamide-Induced Cardiomyopathy. Bull Exp Biol Med 164:483-487 (2018). PubMed: 29504102

    Customer reviews and Q&As

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