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    mmp2-antibody-ab235167.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP
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Anti-MMP2 antibody (ab235167)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab235167)
  • Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (ab235167)
  • Western blot - Anti-MMP2 antibody (ab235167)
  • Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (ab235167)

Key features and details

  • Rabbit polyclonal to MMP2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Protein
Recombinant human MMP2 protein (Active) (ab81550)

View more associated products

Overview

  • Product name

    Anti-MMP2 antibody
    See all MMP2 primary antibodies
  • Description

    Rabbit polyclonal to MMP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HepG2 and U87MG cells. IHC-P: Human metastatic breast carcinoma in lymph node tissue. WB: MMP2 HEK-293 over-expressing lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Matrix Metalloproteinases
    • MMP
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MMPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • MMPs
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • MMPs
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Neuroscience
    • Diseases
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Recombinant Protein

    • Recombinant human MMP2 protein (Active) (ab81550)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab235167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 73 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 15 µg/ml.
Notes
WB
1/1000. Predicted molecular weight: 73 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 15 µg/ml.

Target

  • Function

    Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
    -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
    PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
  • Tissue specificity

    Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
  • Involvement in disease

    Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
    The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
  • Target information above from: UniProt accession P08253 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 282872 Cow
    • Entrez Gene: 4313 Human
    • Entrez Gene: 17390 Mouse
    • Entrez Gene: 100009000 Rabbit
    • Entrez Gene: 81686 Rat
    • Omim: 120360 Human
    • SwissProt: Q9GLE5 Cow
    • SwissProt: P08253 Human
    • SwissProt: P33434 Mouse
    • SwissProt: P50757 Rabbit
    • SwissProt: P33436 Rat
    • Unigene: 513617 Human
    • Unigene: 29564 Mouse
    • Unigene: 6422 Rat
    see all
  • Alternative names

    • 72 kDa gelatinase antibody
    • 72kD type IV collagenase antibody
    • CLG 4 antibody
    • CLG 4A antibody
    • CLG4 antibody
    • CLG4A antibody
    • Collagenase Type 4 alpha antibody
    • Collagenase type IV A antibody
    • Gelatinase A antibody
    • Gelatinase alpha antibody
    • Gelatinase neutrophil antibody
    • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
    • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 2 antibody
    • Matrix metalloproteinase II antibody
    • Matrix metalloproteinase-2 antibody
    • MMP 2 antibody
    • MMP II antibody
    • MMP-2 antibody
    • MMP2 antibody
    • MMP2_HUMAN antibody
    • MONA antibody
    • Neutrophil gelatinase antibody
    • PEX antibody
    • TBE 1 antibody
    • TBE-1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab235167)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab235167)

    Immunohistochemical analysis of paraffin-embedded human metastic breast carcinoma tissue in lymph node tissue labeling MMP2 with ab235167 at 1/100 dilution (30 minutes at RT), followed by a ready to use Goat anti-Rabbit polyclonal IgG (HRP) (8 minutes at RT). Counterstained with hematoxylin.

    Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.

  • Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (ab235167)
    Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (ab235167)

    Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling MMP2 with ab235167 at 15 µg/ml (Overnight at 4°C), followed by a Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 15 µg/ml (1 hour at RT) (green).
    Panel A: DAPI.
    Panel B: ab235167 + secondary antibody.
    Panel C: Merge of panel A and B.
    Panel D: Secondary only control.

  • Western blot - Anti-MMP2 antibody (ab235167)
    Western blot - Anti-MMP2 antibody (ab235167)
    All lanes : Anti-MMP2 antibody (ab235167) at 1/1000 dilution

    Lane 1 : MMP2 HEK-293 (human epithelial cell line from embryonic kidney) over-expressing lysate
    Lane 2 : HEK-293 (empty vector) lysate

    Secondary
    All lanes : Pre-adsorbed goat anti-rabbit IgG (H&L) conjugated to HRP at 1/70000 dilution

    Predicted band size: 73 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% BLOTTO.

  • Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (ab235167)
    Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (ab235167)

    Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized U87MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling MMP2 with ab235167 at 15 µg/ml (Overnight at 4°C), followed by a Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 5 µg/ml (1 hour at RT) (green).
    Panel A: DAPI.
    Panel B: ab235167 + secondary antibody.
    Panel C: Merge of panel A and B.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (2)

Publishing research using ab235167? Please let us know so that we can cite the reference in this datasheet.

ab235167 has been referenced in 2 publications.

  • Guo X  et al. LncRNA GAS6 antisense RNA 1 facilitates the tumorigenesis of clear cell renal cell carcinoma by regulating the AMP-activated protein kinase/mTOR signaling pathway. Oncol Lett 22:727 (2021). PubMed: 34429767
  • Li R  et al. Hepsin Promotes Epithelial-Mesenchymal Transition and Cell Invasion Through the miR-222/PPP2R2A/AKT Axis in Prostate Cancer. Onco Targets Ther 13:12141-12149 (2020). PubMed: 33268993

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