Overview

  • Product name

  • Description

    Rabbit polyclonal to MMP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • ICC/IF: HepG2 and U87MG cells. IHC-P: Human metastatic breast carcinoma in lymph node tissue. WB: MMP2 HEK-293 over-expressing lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab235167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 73 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 15 µg/ml.

Target

  • Function

    Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
    -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
    PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
  • Tissue specificity

    Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
  • Involvement in disease

    Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
    The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
  • Information by UniProt
  • Database links

  • Alternative names

    • 72 kDa gelatinase antibody
    • 72kD type IV collagenase antibody
    • CLG 4 antibody
    • CLG 4A antibody
    • CLG4 antibody
    • CLG4A antibody
    • Collagenase Type 4 alpha antibody
    • Collagenase type IV A antibody
    • Gelatinase A antibody
    • Gelatinase alpha antibody
    • Gelatinase neutrophil antibody
    • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
    • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 2 antibody
    • Matrix metalloproteinase II antibody
    • Matrix metalloproteinase-2 antibody
    • MMP 2 antibody
    • MMP II antibody
    • MMP-2 antibody
    • MMP2 antibody
    • MMP2_HUMAN antibody
    • MONA antibody
    • Neutrophil gelatinase antibody
    • PEX antibody
    • TBE 1 antibody
    • TBE-1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human metastic breast carcinoma tissue in lymph node tissue labeling MMP2 with ab235167 at 1/100 dilution (30 minutes at RT), followed by a ready to use Goat anti-Rabbit polyclonal IgG (HRP) (8 minutes at RT). Counterstained with hematoxylin.

    Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.

  • Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling MMP2 with ab235167 at 15 µg/ml (Overnight at 4°C), followed by a Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 15 µg/ml (1 hour at RT) (green).
    Panel A: DAPI.
    Panel B: ab235167 + secondary antibody.
    Panel C: Merge of panel A and B.
    Panel D: Secondary only control.

  • All lanes : Anti-MMP2 antibody (ab235167) at 1/1000 dilution

    Lane 1 : MMP2 HEK-293 (human epithelial cell line from embryonic kidney) over-expressing lysate
    Lane 2 : HEK-293 (empty vector) lysate

    Secondary
    All lanes : Pre-adsorbed goat anti-rabbit IgG (H&L) conjugated to HRP at 1/70000 dilution

    Predicted band size: 73 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% BLOTTO.

  • Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized U87MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling MMP2 with ab235167 at 15 µg/ml (Overnight at 4°C), followed by a Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 5 µg/ml (1 hour at RT) (green).
    Panel A: DAPI.
    Panel B: ab235167 + secondary antibody.
    Panel C: Merge of panel A and B.

References

ab235167 has not yet been referenced specifically in any publications.

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