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  1. Link

    mmp2-antibody-ab97779.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP
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Anti-MMP2 antibody (ab97779)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (123)

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Western blot - Anti-MMP2 antibody (ab97779)
  • Western blot - Anti-MMP2 antibody (ab97779)
  • Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (ab97779)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab97779)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab97779)
  • Immunoprecipitation - Anti-MMP2 antibody (ab97779)

Key features and details

  • Rabbit polyclonal to MMP2
  • Suitable for: IHC-P, IP, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-MMP2 antibody [EPR1184] (ab92536)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-MMP2 antibody
    See all MMP2 primary antibodies
  • Description

    Rabbit polyclonal to MMP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Sheep, Rabbit, Cow, Pig, Xenopus laevis
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 193 and 545 of Human MMP2 (NP_004521).

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lysate; U-87 MG whole cell extracts. PC-3, mouse brain.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Matrix Metalloproteinases
    • MMP
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MMPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • MMPs
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • MMPs
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Diseases
    • Neuroscience
    • Processes

Associated products

  • Assay kits

    • MMP2 Inhibitor Screening Assay Kit (Colorimetric) (ab139446)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human MMP2 protein (Active) (ab81550)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
1/100 - 1/1000.
IP
1/100 - 1/500.
ICC/IF
1/100 - 1/1000.
WB (2)
1/500 - 1/3000. Predicted molecular weight: 74 kDa.
Notes
IHC-P
1/100 - 1/1000.
IP
1/100 - 1/500.
ICC/IF
1/100 - 1/1000.
WB
1/500 - 1/3000. Predicted molecular weight: 74 kDa.

Target

  • Function

    Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
    -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
    PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
  • Tissue specificity

    Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
  • Involvement in disease

    Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
    The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
  • Target information above from: UniProt accession P08253 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 282872 Cow
    • Entrez Gene: 4313 Human
    • Entrez Gene: 397391 Pig
    • Entrez Gene: 100009000 Rabbit
    • Entrez Gene: 81686 Rat
    • Entrez Gene: 380389 Xenopus laevis
    • Omim: 120360 Human
    • SwissProt: Q9GLE5 Cow
    • SwissProt: P08253 Human
    • SwissProt: P50757 Rabbit
    • SwissProt: P33436 Rat
    • Unigene: 513617 Human
    • Unigene: 6422 Rat
    see all
  • Alternative names

    • 72 kDa gelatinase antibody
    • 72kD type IV collagenase antibody
    • CLG 4 antibody
    • CLG 4A antibody
    • CLG4 antibody
    • CLG4A antibody
    • Collagenase Type 4 alpha antibody
    • Collagenase type IV A antibody
    • Gelatinase A antibody
    • Gelatinase alpha antibody
    • Gelatinase neutrophil antibody
    • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
    • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 2 antibody
    • Matrix metalloproteinase II antibody
    • Matrix metalloproteinase-2 antibody
    • MMP 2 antibody
    • MMP II antibody
    • MMP-2 antibody
    • MMP2 antibody
    • MMP2_HUMAN antibody
    • MONA antibody
    • Neutrophil gelatinase antibody
    • PEX antibody
    • TBE 1 antibody
    • TBE-1 antibody
    see all

Images

  • Western blot - Anti-MMP2 antibody (ab97779)
    Western blot - Anti-MMP2 antibody (ab97779)
    Anti-MMP2 antibody (ab97779) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 74 kDa



    7.5% SDS Page
  • Western blot - Anti-MMP2 antibody (ab97779)
    Western blot - Anti-MMP2 antibody (ab97779)
    All lanes : Anti-MMP2 antibody (ab97779) at 1/3000 dilution

    Lane 1 : PC-3
    Lane 2 : U-87 MG
    Lane 3 : SK-N-SH

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 74 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (ab97779)
    Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (ab97779)

    Immunofluorescent analysis of methanol-fixed HeLa (Human epithelial cells from cervix adenocarcinoma cell line) labeling MMP2 with ab97779 at 1/200 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab97779)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab97779)

    Immunohistochemical analysis of Human endometrical carcinoma labeling MMP2 with ab97779 at 1/500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab97779)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab97779)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MMP2 with ab97779 at 1/500 dilution.

  • Immunoprecipitation - Anti-MMP2 antibody (ab97779)
    Immunoprecipitation - Anti-MMP2 antibody (ab97779)

    MMP2 was immunoprecipitated from U-87 MG (glioblastoma-astrocytoma epithelial cell line) whole cell extracts with 5 µg ab97779. Western blot was performed from the immunoprecipitate using ab97779.

    Lane 1: Control IgG IP in U-87 MG whole cell extracts.

    Lane 2: ab97779 IP in U-87 MG whole cell extracts.

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (123)

Publishing research using ab97779? Please let us know so that we can cite the reference in this datasheet.

ab97779 has been referenced in 123 publications.

  • Yuan Y  et al. Circ-CCS is identified as a cancer-promoting circRNA in lung cancer partly by regulating the miR-383/E2F7 axis. Life Sci 267:118955 (2021). PubMed: 33359669
  • Huang Y & Feng G MiR-423-5p aggravates lung adenocarcinoma via targeting CADM1. Thorac Cancer 12:210-217 (2021). PubMed: 33205911
  • Leng Y  et al. Overexpression of microRNA-29b inhibits epithelial-mesenchymal transition and angiogenesis of colorectal cancer through the ETV4/ERK/EGFR axis. Cancer Cell Int 21:17 (2021). PubMed: 33407520
  • Moore MP  et al. A dietary ketone ester mitigates histological outcomes of NAFLD and markers of fibrosis in high-fat diet fed mice. Am J Physiol Gastrointest Liver Physiol 320:G564-G572 (2021). PubMed: 33501889
  • Shi X  et al. Naringenin inhibits migration, invasion, induces apoptosis in human lung cancer cells and arrests tumour progression in vitro. J Cell Mol Med 25:2563-2571 (2021). PubMed: 33523599
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MMP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 20 2021

Western blot abreview for Anti-MMP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Chicken Purified protein (eye tissues)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
20 µg
Specification
eye tissues
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 07 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MMP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (urinary bladder)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate 6
Permeabilization
No
Specification
urinary bladder
Blocking step
serum free protein block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 23°C
Fixative
10% NBF
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 17 2019

Western blot abreview for Anti-MMP2 antibody

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-15%)
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 05 2014

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-MMP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Antigen retrieval step
None
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 03 2014

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