Anti-MMP2 antibody (ab97779)
Overview
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Product name
Anti-MMP2 antibody
See all MMP2 primary antibodies -
Description
Rabbit polyclonal to MMP2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Cow, Pig, Xenopus laevis
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Immunogen
Recombinant protein fragment corresponding to a region within amino acids 193 and 545 of Human MMP2 (NP_004521).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lysate; U-87 MG whole cell extracts. PC-3, mouse brain.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab97779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| IP | 1/100 - 1/500. | |
| ICC/IF | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 74 kDa. |
Target
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Function
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. -
Tissue specificity
Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. -
Involvement in disease
Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsPhosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3. -
Cellular localization
Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes. - Information by UniProt
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Database links
- Entrez Gene: 282872 Cow
- Entrez Gene: 4313 Human
- Entrez Gene: 17390 Mouse
- Entrez Gene: 397391 Pig
- Entrez Gene: 100009000 Rabbit
- Entrez Gene: 81686 Rat
- Entrez Gene: 380389 Xenopus laevis
- Omim: 120360 Human
see all -
Alternative names
- 72 kDa gelatinase antibody
- 72kD type IV collagenase antibody
- CLG 4 antibody
see all
Images
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Western blot - Anti-MMP2 antibody (ab97779)Anti-MMP2 antibody (ab97779) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 74 kDa
7.5% SDS Page -
Western blot - Anti-MMP2 antibody (ab97779)All lanes : Anti-MMP2 antibody (ab97779) at 1/3000 dilution
Lane 1 : PC-3
Lane 2 : U-87 MG
Lane 3 : SK-N-SH
Lysates/proteins at 30 µg per lane.
Predicted band size: 74 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (ab97779)Immunofluorescent analysis of methanol-fixed HeLa (Human epithelial cells from cervix adenocarcinoma cell line) labeling MMP2 with ab97779 at 1/200 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab97779)Immunohistochemical analysis of Human endometrical carcinoma labeling MMP2 with ab97779 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab97779)Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MMP2 with ab97779 at 1/500 dilution.
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Immunoprecipitation - Anti-MMP2 antibody (ab97779)MMP2 was immunoprecipitated from U-87 MG (glioblastoma-astrocytoma epithelial cell line) whole cell extracts with 5 µg ab97779. Western blot was performed from the immunoprecipitate using ab97779.
Lane 1: Control IgG IP in U-87 MG whole cell extracts.
Lane 2: ab97779 IP in U-87 MG whole cell extracts.
Protocols
References
ab97779 has been referenced in 16 publications.
- Zhao J et al. Quercetin inhibits cell viability, migration and invasion by regulating miR-16/HOXA10 axis in oral cancer. Eur J Pharmacol 847:11-18 (2019). PubMed: 30639311
- Cui C et al. Znhit1 inhibits breast cancer by up-regulating PTEN to deactivate the PI3K/Akt/mTOR pathway. Life Sci 224:204-211 (2019). PubMed: 30928405
- Cai F et al. CXCL12-regulated miR-370-3p functions as a tumor suppressor gene by targeting HMGA2 in nonfunctional pituitary adenomas. Mol Cell Endocrinol 488:25-35 (2019). PubMed: 30853598
- Liu P et al. LncRNA-MALAT1 promotes neovascularization in diabetic retinopathy through regulating miR-125b/VE-cadherin axis. Biosci Rep 39:N/A (2019). PubMed: 30988072
- Wang J et al. CIAPIN1 Targeted NHE1 and ERK1/2 to Suppress NSCLC Cells' Metastasis and Predicted Good Prognosis in NSCLC Patients Receiving Pulmonectomy. Oxid Med Cell Longev 2019:1970818 (2019). PubMed: 31093311
