Product nameAnti-MMP2 antibody
See all MMP2 primary antibodies
DescriptionRabbit polyclonal to MMP2
Tested applicationsSuitable for: IHC-P, IP, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Cow, Pig, Xenopus laevis
Recombinant protein fragment corresponding to a region within amino acids 193 and 545 of Human MMP2 (NP_004521).
- 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lysate; U-87 MG whole cell extracts. PC-3, mouse brain.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 74 kDa.|
FunctionUbiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
Tissue specificityProduced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
Involvement in diseaseDefects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsPhosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
Cellular localizationSecreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
- Information by UniProt
- 72 kDa gelatinase antibody
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Anti-MMP2 antibody (ab97779) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 74 kDa
7.5% SDS Page
All lanes : Anti-MMP2 antibody (ab97779) at 1/3000 dilution
Lane 1 : PC-3
Lane 2 : U-87 MG
Lane 3 : SK-N-SH
Lysates/proteins at 30 µg per lane.
Predicted band size: 74 kDa
Immunofluorescent analysis of methanol-fixed HeLa (Human epithelial cells from cervix adenocarcinoma cell line) labeling MMP2 with ab97779 at 1/200 dilution.
Immunohistochemical analysis of Human endometrical carcinoma labeling MMP2 with ab97779 at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MMP2 with ab97779 at 1/500 dilution.
MMP2 was immunoprecipitated from U-87 MG (glioblastoma-astrocytoma epithelial cell line) whole cell extracts with 5 µg ab97779. Western blot was performed from the immunoprecipitate using ab97779.
Lane 1: Control IgG IP in U-87 MG whole cell extracts.
Lane 2: ab97779 IP in U-87 MG whole cell extracts.
ab97779 has been referenced in 16 publications.
- Zhao J et al. Quercetin inhibits cell viability, migration and invasion by regulating miR-16/HOXA10 axis in oral cancer. Eur J Pharmacol 847:11-18 (2019). PubMed: 30639311
- Cui C et al. Znhit1 inhibits breast cancer by up-regulating PTEN to deactivate the PI3K/Akt/mTOR pathway. Life Sci 224:204-211 (2019). PubMed: 30928405
- Cai F et al. CXCL12-regulated miR-370-3p functions as a tumor suppressor gene by targeting HMGA2 in nonfunctional pituitary adenomas. Mol Cell Endocrinol 488:25-35 (2019). PubMed: 30853598
- Liu P et al. LncRNA-MALAT1 promotes neovascularization in diabetic retinopathy through regulating miR-125b/VE-cadherin axis. Biosci Rep 39:N/A (2019). PubMed: 30988072
- Wang J et al. CIAPIN1 Targeted NHE1 and ERK1/2 to Suppress NSCLC Cells' Metastasis and Predicted Good Prognosis in NSCLC Patients Receiving Pulmonectomy. Oxid Med Cell Longev 2019:1970818 (2019). PubMed: 31093311