Recombinant
RabMAb

Recombinant Anti-MMP2 antibody [EPR1184] - Low endotoxin, Azide free (ab215986)

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Overview

  • Product name

    Anti-MMP2 antibody [EPR1184] - Low endotoxin, Azide free
    See all MMP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR1184] to MMP2 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MMP2 aa 550-650 (C terminal).
    Database link: P08253

  • Positive control

    • L6, fetal heart and NIH/3T3 cell lysates

  • General notes

    ab215986 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab215986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 74 kDa.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
      -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
      PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.

    • Tissue specificity

      Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.

    • Involvement in disease

      Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.

    • Sequence similarities

      Belongs to the peptidase M10A family.
      Contains 3 fibronectin type-II domains.
      Contains 4 hemopexin-like domains.

    • Domain

      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

    • Post-translational
      modifications

      Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
      The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.

    • Cellular localization

      Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 72 kDa gelatinase antibody
      • 72kD type IV collagenase antibody
      • CLG 4 antibody
      • CLG 4A antibody
      • CLG4 antibody
      • CLG4A antibody
      • Collagenase Type 4 alpha antibody
      • Collagenase type IV A antibody
      • Gelatinase A antibody
      • Gelatinase alpha antibody
      • Gelatinase neutrophil antibody
      • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
      • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody
      • Matrix Metalloproteinase 2 antibody
      • Matrix metalloproteinase II antibody
      • Matrix metalloproteinase-2 antibody
      • MMP 2 antibody
      • MMP II antibody
      • MMP-2 antibody
      • MMP2 antibody
      • MMP2_HUMAN antibody
      • MONA antibody
      • Neutrophil gelatinase antibody
      • PEX antibody
      • TBE 1 antibody
      • TBE-1 antibody
      see all

    Images

    • Flow Cytometry - Anti-MMP2 antibody [EPR1184] - Low endotoxin, Azide free (ab215986)
      Flow Cytometry - Anti-MMP2 antibody [EPR1184] - Low endotoxin, Azide free (ab215986)

      Flow Cytometry analysis of PC-3 (human prostate adenocarcinoma) cells labeling MMP2 with purified ab92536 at 1/180 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92536).

    • Flow Cytometry - Anti-MMP2 antibody [EPR1184] - Low endotoxin, Azide free (ab215986)
      Flow Cytometry - Anti-MMP2 antibody [EPR1184] - Low endotoxin, Azide free (ab215986)
      Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab92536 at a dilution of 1 in 400 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92536).

    • Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody [EPR1184] - Low endotoxin, Azide free (ab215986)
      Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody [EPR1184] - Low endotoxin, Azide free (ab215986)

      Immunofluorescence staining of PC-3 cells with purified ab92536 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab92536 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92536).

    References

    This product has been referenced in:

    • Li C  et al. Inhibitory Effects of Isorhamnetin on the Invasion of Human Breast Carcinoma Cells by Downregulating the Expression and Activity of Matrix Metalloproteinase-2/9. Nutr Cancer 67:1191-200 (2015). Human . Read more (PubMed: 26359917) »
    • She S  et al. C-reactive protein is a biomarker of AFP-negative HBV-related hepatocellular carcinoma. Int J Oncol 47:543-54 (2015). Read more (PubMed: 26058824) »
    See all 7 Publications for this product

    Publishing research using ab215986? Please let us know so that we can cite the reference in this datasheet.

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