Recombinant Anti-MMP2 antibody [EPR17003-25] (ab181286)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17003-25] to MMP2
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MMP2 antibody [EPR17003-25]
See all MMP2 primary antibodies -
Description
Rabbit monoclonal [EPR17003-25] to MMP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: HT1080 whole cell lysate. WB: Human, mouse and rat plasma lysates; Rat serum lysate; Mouse and rat lung lysates; HT1080 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17003-25 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa).
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IP |
1/30.
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Notes |
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WB
1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa). |
IP
1/30. |
Target
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Function
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. -
Tissue specificity
Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. -
Involvement in disease
Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsPhosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3. -
Cellular localization
Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes. - Information by UniProt
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Database links
- Entrez Gene: 4313 Human
- Entrez Gene: 17390 Mouse
- Entrez Gene: 81686 Rat
- Omim: 120360 Human
- SwissProt: P08253 Human
- SwissProt: P33434 Mouse
- SwissProt: P33436 Rat
- Unigene: 513617 Human
see all -
Alternative names
- 72 kDa gelatinase antibody
- 72kD type IV collagenase antibody
- CLG 4 antibody
see all
Images
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MMP2 was immunoprecipitated from 0.35 mg HT1080 (human fibrosarcoma cell line) whole cell lysate with ab181286 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181286 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HT1080 whole cell lysate 10 µg (Input).
Lane 2: ab181286 IP in HT1080 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab181286 in HT1080 whole cell lysate (-).Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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Lanes 1-5 : Anti-MMP2 antibody [EPR17003-25] (ab181286) at 1/1000 dilution
Lanes 6-7 : Anti-MMP2 antibody [EPR17003-25] (ab181286) at 1/2000 dilution
Lane 1 : Mouse plasma lysate at 20 µg
Lane 2 : Rat serum lysate at 20 µg
Lane 3 : Mouse lung lysate at 20 µg
Lane 4 : Rat plasma lysate at 20 µg
Lane 5 : Rat lung lysate at 20 µg
Lane 6 : HT1080 (human fibrosarcoma cell line) whole cell lysate at 10 µg
Lane 7 : Human plasma lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 73 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?Exposure time: Lanes 1-2: 3 minutes; Lanes 3-5: 10 seconds; Lanes 6-7: 30 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (20)
ab181286 has been referenced in 20 publications.
- Tang J et al. Circ_0006220 Contributes to NSCLC Progression through miR-342-3p/GOT2 Axis. Ann Thorac Cardiovasc Surg 29:11-22 (2023). PubMed: 36575008
- Li C et al. miR-362-3p suppresses ovarian cancer by inhibiting LRP8. Transl Oncol 15:101284 (2022). PubMed: 34839107
- Fu X et al. Molecular mechanism of albumin in suppressing invasion and metastasis of hepatocellular carcinoma. Liver Int 42:696-709 (2022). PubMed: 34854209
- Huang H et al. Eriodictyol suppresses the malignant progression of colorectal cancer by downregulating tissue specific transplantation antigen P35B (TSTA3) expression to restrain fucosylation. Bioengineered 13:5551-5563 (2022). PubMed: 35184647
- Lv B et al. Network Pharmacology Experiments Show That Emodin Can Exert a Protective Effect on MCAO Rats by Regulating Hif-1α/VEGF-A Signaling. ACS Omega 7:22577-22593 (2022). PubMed: 35811865