Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate (12 x 8 well strips)
- Sample type: Inhibitor compounds
Product nameMMP2 Inhibitor Screening Kit (Fluorometric)
See all MMP2 kits
Sample typeInhibitor compounds
Assay durationMultiple steps standard assay
MMP-2 Inhibitor Screening Kit (ab283367) provides a quick and sensitive way for screening, studying and characterizing potential inhibitors of MMP-2. In this assay, MMP-2 hydrolyzes a FRETbased MMP-2 substrate and releases the quenched fluorescent group, Mca, which can then be detected fluorometrically at Ex/Em = 325/393 nm. A potent, specific MMP-2 inhibitor is also included in the kit.
This product is manufactured by BioVision, an Abcam company and was previously called K2017 MMP-2 Inhibitor Screening Kit (Fluorometric). K2017-100 is the same size as the 100 test size of ab283367.
Matrix metalloproteinase-2 (MMP-2) is a zinc-dependent endopeptidase family member that is capable of degrading denatured collagen, collagen type IV as well as other extracellular matrix proteins in normal physiological processes such as embryonic development, tissue remodeling and in disease processes including metastasis, atherosclerosis, cardiac dysfunction etc. MMP-2 is widely expressed in almost all cell types. Like most MMP's, MMP-2 is secreted as inactive pro-protein that is activated when cleaved by extracellular proteases. Additionally, MMP-2 can be activated intracellularly via S-Glutathionylation.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Inhibitor (NNGH, 2 mM) Yellow cap 1 x 50µl MMP-2 Assay Buffer WM cap 1 x 25ml MMP-2 Substrate Red cap 1 x 100µl Recombinant MMP-2 Blue Cap 1 vial
FunctionUbiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
Tissue specificityProduced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
Involvement in diseaseDefects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsPhosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
Cellular localizationSecreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
- Information by UniProt
- 72 kDa gelatinase
- 72kD type IV collagenase
- CLG 4
ab283367 has not yet been referenced specifically in any publications.