Overview

  • Product name

  • Description

    Rabbit polyclonal to MMP20
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human MMP20 (internal sequence). Gene Accession: NP_004762.
    Database link: O60882

  • Positive control

    • Lysates from 823 cell, mouse brain and Human fetal brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab198815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 54 kDa.

Target

  • Function

    Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation.
  • Tissue specificity

    Expressed specifically in the enamel organ.
  • Involvement in disease

    Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2) [MIM:612529]. AI2A2 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel.
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    Autoactivates at least at the 107-Asn-
    -Tyr-108 site.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI2A2 antibody
    • Enamel metalloproteinase antibody
    • Enamelysin antibody
    • Matrix metalloproteinase 20 antibody
    • Matrix metalloproteinase-20 antibody
    • MMP 20 antibody
    • MMP-20 antibody
    • MMP20 antibody
    • MMP20_HUMAN antibody
    see all

Images

  • All lanes : Anti-MMP20 antibody (ab198815) at 1/400 dilution

    Lane 1 : 823 cell lysate
    Lane 2 : mouse brain tissue lysate
    Lane 3 : Human fetal brain tissue lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 54 kDa


    Exposure time: 20 seconds


    Gel: 8%SDS-PAGE

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MMP20 with ab198815 at 1/40 dilution.

References

ab198815 has not yet been referenced specifically in any publications.

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