Key features and details
- Rabbit polyclonal to MMP20
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MMP20 antibody
See all MMP20 primary antibodies
DescriptionRabbit polyclonal to MMP20
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human MMP20 (internal sequence). Gene Accession: NP_004762.
Database link: O60882
- Lysates from 823 cell, mouse brain and Human fetal brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab198815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 54 kDa.|
FunctionDegrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation.
Tissue specificityExpressed specifically in the enamel organ.
Involvement in diseaseDefects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2) [MIM:612529]. AI2A2 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsAutoactivates at least at the 107-Asn-
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- AI2A2 antibody
- Enamel metalloproteinase antibody
- Enamelysin antibody
All lanes : Anti-MMP20 antibody (ab198815) at 1/400 dilution
Lane 1 : 823 cell lysate
Lane 2 : mouse brain tissue lysate
Lane 3 : Human fetal brain tissue lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 54 kDa
Exposure time: 20 seconds
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MMP20 with ab198815 at 1/40 dilution.
ab198815 has been referenced in 1 publication.
- Wang S et al. Matrix Metalloproteinase Expressions Play Important role in Prediction of Ovarian Cancer Outcome. Sci Rep 9:11677 (2019). PubMed: 31406154