Overview

  • Product name

    Anti-MMP20 antibody [EP1275Y]
    See all MMP20 primary antibodies
  • Description

    Rabbit monoclonal [EP1275Y] to MMP20
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MMP20 aa 450-550 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa whole cell lysate (ab150035); HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation.
    • Tissue specificity

      Expressed specifically in the enamel organ.
    • Involvement in disease

      Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2) [MIM:612529]. AI2A2 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel.
    • Sequence similarities

      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • Domain

      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications

      Autoactivates at least at the 107-Asn-
      -Tyr-108 site.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • AI2A2 antibody
      • Enamel metalloproteinase antibody
      • Enamelysin antibody
      • Matrix metalloproteinase 20 antibody
      • Matrix metalloproteinase-20 antibody
      • MMP 20 antibody
      • MMP-20 antibody
      • MMP20 antibody
      • MMP20_HUMAN antibody
      see all

    Images

    • Anti-MMP20 antibody [EP1275Y] (ab76109) at 1/500 dilution + HeLa cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 54 kDa
      Observed band size: 54 kDa

    References

    This product has been referenced in:

    • Li Z  et al. Bcl11b regulates enamel matrix protein expression and dental epithelial cell differentiation during rat tooth development. Mol Med Rep 15:297-304 (2017). Read more (PubMed: 27959403) »
    • Yang T  et al. High amounts of fluoride induce apoptosis/cell death in matured ameloblast-like LS8 cells by downregulating Bcl-2. Arch Oral Biol 58:1165-73 (2013). WB ; Mouse . Read more (PubMed: 23598055) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Answer

    Merci de nous avoir contactés.

    Nous sommes désolés d'apprendre que le produit ab76109que vous avez reçu ne fonctionne pas comme attendu.
    Comme convenu ce matin par téléphone, j'ai mis en place l'envoi d'une unité de ab76109 d'un lot différent de celui que vous avez originalement reçu. Le numéro de commande de remplacement gratuit de ce produit est : *******. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

    N'hésitez pas àme contacter lors d'une prochaine occasion.

    Bonne continuation dans vos recherches.

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