Recombinant
RabMAb

Anti-MMP20 antibody [EP1275Y] (ab76109)

Overview

  • Product name
    Anti-MMP20 antibody [EP1275Y]
    See all MMP20 primary antibodies
  • Description
    Rabbit monoclonal [EP1275Y] to MMP20
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MMP20 aa 450-550 (C terminal). The exact sequence is proprietary.

  • Positive control
    • HeLa cell lysate; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation.
    • Tissue specificity
      Expressed specifically in the enamel organ.
    • Involvement in disease
      Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2) [MIM:612529]. AI2A2 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel.
    • Sequence similarities
      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • Domain
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications
      Autoactivates at least at the 107-Asn-
      -Tyr-108 site.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI2A2 antibody
      • Enamel metalloproteinase antibody
      • Enamelysin antibody
      • Matrix metalloproteinase 20 antibody
      • Matrix metalloproteinase-20 antibody
      • MMP 20 antibody
      • MMP-20 antibody
      • MMP20 antibody
      • MMP20_HUMAN antibody
      see all

    Images

    • ab76109 at 1/100-1/250 dilution staining MMP20 in HeLa cells by Immunofluorescence.
    • Anti-MMP20 antibody [EP1275Y] (ab76109) at 1/500 dilution + HeLa cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 54 kDa
      Observed band size: 54 kDa

    References

    This product has been referenced in:
    • Yang T  et al. High amounts of fluoride induce apoptosis/cell death in matured ameloblast-like LS8 cells by downregulating Bcl-2. Arch Oral Biol 58:1165-73 (2013). WB ; Mouse . Read more (PubMed: 23598055) »
    • Mohazab L  et al. Critical role for avß6 integrin in enamel biomineralization. J Cell Sci 126:732-44 (2013). Read more (PubMed: 23264742) »

    See all 2 Publications for this product

    Customer reviews and Q&As

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