Key features and details
- Rabbit polyclonal to MMP22
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
Product nameAnti-MMP22 antibody
See all MMP22 primary antibodies
DescriptionRabbit polyclonal to MMP22
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Rat, Cow
Recombinant full length protein (His-T7-tag) corresponding to Human MMP22 aa 79-390. Expressed in E. coli. N-terminal tags. Full length soluble chain.
YTLTPARLRWDHFNLTYRILSFPRNLLSPRETRRALAAAFRMWSDVSPFS FREVAPEQPSDLRIGFYPINHTDCLVSALHHCFDGPTGELAHAFFPPHGG IHFDDSEYWVLGPTRYSWKKGVWLTDLVHVAAHEIGHALGLMHSQHGRAL MHLNATLRGWKALSQDELWGLHRLYGCLDRLFVCASWARRGFCDARRRLM KRLCPSSCDFCYEFPFPTVATTPPPPRTKTRLVPEGRNVTFRCGQKILHK KGKVYWYKDQEPLEFSYPGYLALGEAHLSIIANAVNEGTYTCVVRRQQRV LTTYSWRVRVRG
Database link: O75900-1
- IHC-P: Human breast cancer, glioma, prostate cancer, prostate, stomach and colorectal cancer tissue. WB: PC-3 cell lysate. Pig stomach and large intestine tissue lysate. Recombinant human MMP22 protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography
Our Abpromise guarantee covers the use of ab231617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 44 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionProtease. May regulate the surface expression of some potassum channels by retaining them in the endoplasmic reticulum.
Tissue specificityPredominantly expressed in ovary, testis and prostate.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
DomainThe toxin-like domain associates with, and blocks several potassium channels in the nanomolar to low micromolar range. The relative affinity is Kv1.6 > Kv1.3 > Kv1.1 = Kv3.2 > Kv1.4.
Proteolytic cleavage might yield an active form.
Cellular localizationEndoplasmic reticulum membrane. Membrane. A secreted form produced by proteolytic cleavage may also exist.
- Information by UniProt
- Femalysin antibody
- Matrix metallopeptidase 21 antibody
- matrix metallopeptidase 23B antibody
Anti-MMP22 antibody (ab231617) at 2 µg/ml + Recombinant human MMP22 protein
Predicted band size: 44 kDa
All lanes : Anti-MMP22 antibody (ab231617) at 1 µg/ml
Lane 1 : PC-3 (Human prostate adenocarcinoma cell line) cell lysate
Lane 2 : Pig stomach tissue lysate
Lane 3 : Pig large intestine tissue lysate
All lanes : HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 44 kDa
Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for MMP22 with ab231617 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for MMP22 with ab231617 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human stomach tissue stained for MMP22 with ab231617 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human glioma tissue stained for MMP22 with ab231617 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for MMP22 with ab231617 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human prostate tissue stained for MMP22 with ab231617 at 30 µg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231617 has not yet been referenced specifically in any publications.