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    mmp24-antibody-ab233004.pdf

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Anti-MMP24 antibody (ab233004)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)
  • Western blot - Anti-MMP24 antibody (ab233004)
  • Western blot - Anti-MMP24 antibody (ab233004)
  • Western blot - Anti-MMP24 antibody (ab233004)
  • Western blot - Anti-MMP24 antibody (ab233004)
  • Western blot - Anti-MMP24 antibody (ab233004)

Key features and details

  • Rabbit polyclonal to MMP24
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-MMP24 antibody
  • Description

    Rabbit polyclonal to MMP24
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment (His-T7-tag) corresponding to Human MMP24 aa 429-642. Two N-terminal tags. (Expressed in E.coli).
    Sequence:

    IDAAYERADGRFVFFKGDKYWVFKEVTVEPGYPHSLGELGSCLPREGIDT ALRWEPVGKTYFFKGERYWRYSEERRATDPGYPKPITVWKGIPQAPQGAF ISKEGYYTYFYKGRDYWKFDNQKLSVEPGYPRNILRDWMGCNQKEVERRK ERRLPQDDVDIMVTINDVPGSVNAVAVVIPCILSLCILVLVYTIFQFKNK TGPQPVTYYKRPVQ


    Database link: Q9Y5R2
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Rat pancreas, cerebrum and kidney lysate; Human liver lysate; Recombinant human MMP24 protein. IHC-P: Human prostate, kidney, skin cancer, colorectal cancer, liver and stomach cancer tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • MMP
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MMPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • MMPs

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Rat brain cerebrum tissue lysate - total protein (ab4033)
  • Recombinant Protein

    • Recombinant human MMP24 protein (ab157081)

Applications

Our Abpromise guarantee covers the use of ab233004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 20 µg/ml.
WB Use a concentration of 0.2 - 2 µg/ml.

Target

  • Function

    Activates progelatinase A. May also be a proteoglycanase involved in degradation of proteoglycans, such as dermatan sulfate and chondroitin sulfate proteoglycans. Cleaves partially fibronectin, but not collagen type I, nor laminin.
  • Tissue specificity

    Predominantly expressed in brain, kidney, pancreas and lung. Overexpressed in a series of brain tumors, including astrocytomas and glioblastomas.
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization

    Cell membrane and Secreted > extracellular space > extracellular matrix. Also shed from cell surface as soluble proteinase, by a proteolytic cleavage.
  • Target information above from: UniProt accession Q9Y5R2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10893 Human
    • Entrez Gene: 17391 Mouse
    • Entrez Gene: 83513 Rat
    • Omim: 604871 Human
    • SwissProt: Q9Y5R2 Human
    • SwissProt: Q9R0S2 Mouse
    • SwissProt: Q99PW6 Rat
    • Unigene: 715494 Human
    • Unigene: 330707 Mouse
    • Unigene: 389325 Mouse
    • Unigene: 3117 Rat
    see all
  • Alternative names

    • Matrix metallopeptidase 24 (membrane inserted) antibody
    • Matrix metallopeptidase 24 antibody
    • Membrane type 5 matrix metalloproteinase antibody
    • Membrane type matrix metalloproteinase 5 antibody
    • Membrane-type matrix metalloproteinase 5 antibody
    • Membrane-type-5 matrix metalloproteinase antibody
    • MMP 24 antibody
    • MMP 25 antibody
    • MMP-24 antibody
    • Mmp24 antibody
    • MMP24_HUMAN antibody
    • MMP25 antibody
    • MT MMP5 antibody
    • MT-MMP 5 antibody
    • MT5 MMP antibody
    • MT5-MMP antibody
    • MT5MMP antibody
    • MTMMP5 antibody
    • Processed matrix metalloproteinase-24 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)

    Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for MMP24 using ab233004 at 10 µg/ml in immunohistochemical analysis. DAB staining. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)

    Formalin-fixed, paraffin-embedded human liver tissue stained for MMP24 using ab233004 at 10 µg/ml in immunohistochemical analysis. DAB staining. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)

    Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for MMP24 using ab233004 at 10 µg/ml in immunohistochemical analysis. DAB staining. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)

    Formalin-fixed, paraffin-embedded human skin cancer tissue stained for MMP24 using ab233004 at 10 µg/ml in immunohistochemical analysis. DAB staining. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)

    Formalin-fixed, paraffin-embedded human kidney tissue stained for MMP24 using ab233004 at 10 µg/ml in immunohistochemical analysis. DAB staining. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP24 antibody (ab233004)

    Formalin-fixed, paraffin-embedded human prostate tissue stained for MMP24 using ab233004 at 10 µg/ml in immunohistochemical analysis. DAB staining. 

  • Western blot - Anti-MMP24 antibody (ab233004)
    Western blot - Anti-MMP24 antibody (ab233004)
    Anti-MMP24 antibody (ab233004) at 1.5 µg/ml + Rat kidney lysate
  • Western blot - Anti-MMP24 antibody (ab233004)
    Western blot - Anti-MMP24 antibody (ab233004)
    Anti-MMP24 antibody (ab233004) at 1.5 µg/ml + Rat cerebrum lysate
  • Western blot - Anti-MMP24 antibody (ab233004)
    Western blot - Anti-MMP24 antibody (ab233004)
    Anti-MMP24 antibody (ab233004) at 2 µg/ml + Human liver lysate
  • Western blot - Anti-MMP24 antibody (ab233004)
    Western blot - Anti-MMP24 antibody (ab233004)
    Anti-MMP24 antibody (ab233004) at 1.5 µg/ml + Rat pancreas lysate
  • Western blot - Anti-MMP24 antibody (ab233004)
    Western blot - Anti-MMP24 antibody (ab233004)
    Anti-MMP24 antibody (ab233004) at 2 µg/ml + Recombinant human MMP24 protein

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab233004? Please let us know so that we can cite the reference in this datasheet.

    ab233004 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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