Anti-MMP24 antibody (ab233004)
Key features and details
- Rabbit polyclonal to MMP24
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-MMP24 antibody -
Description
Rabbit polyclonal to MMP24 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human MMP24 aa 400 to the C-terminus. Two N-terminal tags. (Expressed in E.coli).
Database link: Q9Y5R2 -
Positive control
- WB: Rat pancreas, cerebrum and kidney lysate; Human liver lysate; Recombinant human MMP24 protein. IHC-P: Human prostate, kidney, skin cancer, colorectal cancer, liver and stomach cancer tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
|
WB |
Use a concentration of 0.2 - 2 µg/ml.
|
Notes |
---|
IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. |
Target
-
Function
Activates progelatinase A. May also be a proteoglycanase involved in degradation of proteoglycans, such as dermatan sulfate and chondroitin sulfate proteoglycans. Cleaves partially fibronectin, but not collagen type I, nor laminin. -
Tissue specificity
Predominantly expressed in brain, kidney, pancreas and lung. Overexpressed in a series of brain tumors, including astrocytomas and glioblastomas. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Cell membrane and Secreted > extracellular space > extracellular matrix. Also shed from cell surface as soluble proteinase, by a proteolytic cleavage. - Information by UniProt
-
Database links
- Entrez Gene: 10893 Human
- Entrez Gene: 17391 Mouse
- Entrez Gene: 83513 Rat
- Omim: 604871 Human
- SwissProt: Q9Y5R2 Human
- SwissProt: Q9R0S2 Mouse
- SwissProt: Q99PW6 Rat
- Unigene: 715494 Human
see all -
Alternative names
- Matrix metallopeptidase 24 (membrane inserted) antibody
- Matrix metallopeptidase 24 antibody
- Membrane type 5 matrix metalloproteinase antibody
see all
Images
-
Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for MMP24 using ab233004 at 10 µg/ml in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded human liver tissue stained for MMP24 using ab233004 at 10 µg/ml in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for MMP24 using ab233004 at 10 µg/ml in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded human skin cancer tissue stained for MMP24 using ab233004 at 10 µg/ml in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded human kidney tissue stained for MMP24 using ab233004 at 10 µg/ml in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded human prostate tissue stained for MMP24 using ab233004 at 10 µg/ml in immunohistochemical analysis. DAB staining.
-
Anti-MMP24 antibody (ab233004) at 1.5 µg/ml + Rat kidney lysate
-
Anti-MMP24 antibody (ab233004) at 1.5 µg/ml + Rat cerebrum lysate
-
Anti-MMP24 antibody (ab233004) at 2 µg/ml + Human liver lysate
-
Anti-MMP24 antibody (ab233004) at 1.5 µg/ml + Rat pancreas lysate
-
Anti-MMP24 antibody (ab233004) at 2 µg/ml + Recombinant human MMP24 protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab233004 has not yet been referenced specifically in any publications.