Overview

  • Product name
  • Description
    Rabbit polyclonal to MMP28
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-335 of Human MMP28 (Uniprot ID: Q9H239).

  • Positive control
    • H1299 whole cell lysate; HeLa cells; Human colon carcinoma tissue
  • General notes
    Keep as a concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 59 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Can degrade casein. Could play a role in tissues homeostasis and repair.
  • Tissue specificity
    Expressed at high levels in testes and lung. Low levels are detected in kidney, pancreas and skin. Also expressed in fetal lung, brain, skeletal muscle and kidney. Expressed selectively in keratinocytesis. Widely expressed in several carcinomas as well. Is up-regulated in response to injury in the skin.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epilysin antibody
    • Matrix metalloproteinase 28 antibody
    • Matrix metalloproteinase-28 antibody
    • MM 28 antibody
    • MM28 antibody
    • MMP 25 antibody
    • MMP 28 antibody
    • MMP-28 antibody
    • MMP25 antibody
    • MMP28 antibody
    • MMP28_HUMAN antibody
    see all

Images

  • Anti-MMP28 antibody (ab155507) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 59 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling MMP28 with ab155507 at 1/500 dilution.
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling MMP28 with ab155507 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

References

This product has been referenced in:
  • Zhang J  et al. Overexpression of MMP21 and MMP28 is associated with gastric cancer progression and poor prognosis. Oncol Lett 15:7776-7782 (2018). Read more (PubMed: 29731903) »
  • Wang X  et al. Epilysin is overexpressed in glioblastoma and related to clinical outcome of patients. Med Oncol 32:363 (2015). Read more (PubMed: 25429835) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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