Overview

  • Product name
  • Description
    Rabbit polyclonal to MMP3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within internal sequence amino acids 214-308 of Human MMP3

  • Positive control
    • 293T, A431, H1299, HeLaS3, Molt-4 and Raji cell lysate. HepG2 whole cell lysate (ab7900). HeLa cells, A549 xenograft tissue

Applications

Our Abpromise guarantee covers the use of ab137659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 53 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHDS6 antibody
    • Matrix metalloproteinase 3 antibody
    • Matrix metalloproteinase-3 antibody
    • MGC126102 antibody
    • MGC126103 antibody
    • MGC126104 antibody
    • MMP 3 antibody
    • MMP-3 antibody
    • MMP3 antibody
    • MMP3_HUMAN antibody
    • Proteoglycanase antibody
    • SL-1 antibody
    • SL1 antibody
    • STMY antibody
    • STMY1 antibody
    • STR1 antibody
    • Stromelysin 1 antibody
    • Stromelysin-1 antibody
    • Transin 1 antibody
    • Transin-1 antibody
    see all

Images

  • Anti-MMP3 antibody (ab137659) at 1/500 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 53 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft labelling MMP3 with ab137659 at 1/500 dilution.
  • Immunofluorescence analysis of methanol-fixed HeLa cells labelling MMP3 with ab137659 at 1/200 dilution. Lower panel is costained with Hoechst 33342.

References

This product has been referenced in:
  • Singh S  et al. Interleukin-17 regulates matrix metalloproteinase activity in human pulmonary tuberculosis. J Pathol 244:311-322 (2018). IHC-P ; Human . Read more (PubMed: 29210073) »
  • Maurel J  et al. Phosphorylated-insulin growth factor I receptor (p-IGF1R) and metalloproteinase-3 (MMP3) expression in advanced gastrointestinal stromal tumors (GIST). A GEIS 19 study. Clin Sarcoma Res 6:10 (2016). Read more (PubMed: 27358721) »
See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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