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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP
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MMP3 Inhibitor Screening Assay Kit (Colorimetric) (ab139439)

  • Datasheet
  • SDS
  • Protocol Booklet
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Plot of OD vs. time
  • Inhibition of MMP3 by NNGH (1.3µM)
  • Inhibiton of MMP3 by NNGH
  • Example graph for Km and Vmax determination

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Sample type: Inhibitor compounds

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Overview

  • Product name

    MMP3 Inhibitor Screening Assay Kit (Colorimetric)
    See all MMP3 kits
  • Detection method

    Colorimetric
  • Sample type

    Inhibitor compounds
  • Assay type

    Enzyme activity
  • Product overview

    Abcam's MMP3 Inhibitor Screening Assay Kit (Colorimetric) (ab139439) is a complete assay system designed to screen MMP3 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5). The MMP cleavage site peptide bond is replaced by a thioester bond in the thiopeptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5’-dithiobis(2-nitrobenzoic acid), Ellman’s reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm (ε=13,600 M-1cm-1 at pH 6.0 and above). The assays are performed in a convenient 96-well microplate format

  • Notes

    This kit is useful to screen inhibitors of MMP3, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.

    Thiol inhibitors should not be used with this kit, as they may interfere with the colorimetric assay.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    96-well Clear Microplate (1/2 Volume) 1 unit
    Colorimetric Assay Buffer 1 x 20ml
    MMP Inhibitor 1 x 50µl
    MMP Substrate 1 x 50µl
    MMP3 Enzyme (Human, Recombinant) 1 x 30µl
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Matrix Metalloproteinases
    • MMP
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • MMP
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • ECM enzymes
    • MMPs
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MMPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • MMPs
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • MMPs
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other
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  • Function

    Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P08254 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • CHDS6
    • Matrix metalloproteinase 3
    • Matrix metalloproteinase 3 preproprotein
    • Matrix metalloproteinase-3
    • MGC126102
    • MGC126103
    • MGC126104
    • MMP 3
    • MMP-3
    • MMP3
    • MMP3_HUMAN
    • Proteoglycanase
    • SL-1
    • SL1
    • STMY
    • STMY1
    • STR1
    • Stromelisin 1
    • Stromelysin 1
    • Stromelysin 1 progelatinase
    • Stromelysin-1
    • Transin 1
    • Transin-1
    see all

Associated products

    Images

    • Plot of OD vs. time
      Plot of OD vs. time

      Slope = V= 1.185E-02 OD/min

    • Inhibition of MMP3 by NNGH (1.3µM)
      Inhibition of MMP3 by NNGH (1.3µM)

      control slope = 1.4E-02 OD/min

      inhibitor slope = 1.3E-04 OD/min

      inhibitor % activity remaining = (1.3E-04/1.4E-02) x 100 = 0.9%

    • Inhibiton of MMP3 by NNGH
      Inhibiton of MMP3 by NNGH
    • Example graph for Km and Vmax determination
      Example graph for Km and Vmax determination

      Km = 276 µM

      Vmax = 10.6 pmol/sec

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab139439? Please let us know so that we can cite the reference in this datasheet.

    ab139439 has not yet been referenced specifically in any publications.

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