Key features and details
- Rabbit polyclonal to MMP7
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Product nameAnti-MMP7 antibody
See all MMP7 primary antibodies
DescriptionRabbit polyclonal to MMP7
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human, Pig
Recombinant full length protein (His-tag) corresponding to Human MMP7 aa 1-267. (Expressed in E.coli).
MRLTVLCAVCLLPGSLALPLPQEAGGMSELQWEQAQDYLKRFYLYDSETK NANSLEAKLKEMQKFFGLPITGMLNSRVIEIMQKPRCGVPDVAEYSLFPN SPKWTSKVVTYRIVSYTRDLPHITVDRLVSKALNMWGKEIPLHFRKVVWG TADIMIGFARGAHGDSYPFDGPGNTLAHAFAPGTGLGGDAHFDEDERWTD GSSLGINFLYAATHELGHSLGMGHSSDPNAVMYPTYGNGDPQNFKLSQDD IKGIQKLYGKRSNSRKK
Database link: P09237
- WB: Human urine; Pig lung lysate; Mouse placenta lysate; Recombinant human MMP7 protein. IHC-P: Human kidney tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml.|
FunctionDegrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
Sequence similaritiesBelongs to the peptidase M10A family.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Matrilysin antibody
- Matrin antibody
- Matrix Metalloproteinase 7 antibody
All lanes : Anti-MMP7 antibody (ab232737) at 3 µg/ml
Lane 1 : Human urine
Lane 2 : Pig lung lysate
Lane 3 : Mouse placenta lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Anti-MMP7 antibody (ab232737) at 3 µg/ml + Recombinant human MMP7 protein
HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Formalin-fixed, paraffin-embedded human kidney tissue stained for MMP7 using ab232737 at 20 µg/mL in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232737 has not yet been referenced specifically in any publications.