• Product name
  • Description
    Rabbit polyclonal to MMP7
  • Host species
  • Specificity
    This antibody specifically recognises MMP7, and does not cross react with MMP1, 2, 3, 8, 9, 10 or 16.
  • Tested applications
    Suitable for: WB, ELISA, IP, IHC-Fr, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to C-Terminus of MMP7 (Human).

  • Positive control
    • PMA stimulated A431 whole cell lysate.



Our Abpromise guarantee covers the use of ab5706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: Use at a concentration of 0.1 - 1.0 µg/ml.
    IHC-Fr: Use at an assay dependent dilution (PMID 18499699).
    IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    IP: Use 3-5µg for 106 cells.
    WB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects a band of approximately 28 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
    • Sequence similarities
      Belongs to the peptidase M10A family.
    • Domain
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Matrilysin antibody
      • Matrin antibody
      • Matrix Metalloproteinase 7 antibody
      • Matrix metalloproteinase-7 antibody
      • MMP 7 antibody
      • MMP-7 antibody
      • MMP7 antibody
      • MMP7_HUMAN antibody
      • MPSL1 antibody
      • PUMP 1 antibody
      • Pump 1 protease antibody
      • Pump-1 protease antibody
      • PUMP1 antibody
      • Uterine matrilysin antibody
      • Uterine metalloproteinase antibody
      see all


    • Immunohistochemistry analysis of human prostate carcinoma stained with rabbit polyclonal anti-MMP-7 (ab5706).

    • All lanes : Anti-MMP7 antibody (ab5706)

      Lane 1 : Mouse brain lysate
      Lane 2 : Human MMP-7 over-expressing Hek293 cell lysate
    • ab5706 (2µg/ml) staining MMP7 in human kidney cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasm and nuclei within proximal convoluted tubules.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.


    This product has been referenced in:
    • Diao B  et al. The role of galectin-3 in the tumorigenesis and progression of pituitary tumors. Oncol Lett 15:4919-4925 (2018). Read more (PubMed: 29545898) »
    • Soler A  et al. Elevated 20-HETE in metabolic syndrome regulates arterial stiffness and systolic hypertension via MMP12 activation. J Mol Cell Cardiol 117:88-99 (2018). WB . Read more (PubMed: 29428638) »

    See all 13 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Human Cell lysate - whole cell (stomach cancer cell)
    Loading amount
    25 µg
    stomach cancer cell
    10uM cannabinoid antagonist
    Gel Running Conditions
    Reduced Denaturing (7.5% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Apr 08 2013

    Thanks for your reply.

    If you can provide the PO number or Abcam order reference number associated with the purchase of ab5706 I will be happy to arrange shipment of ab4044 as a replacement.

    Thanks for the additional information!

    I look forward to seeing your next western blotwhere:

    1. Lesspure MMP7 is loaded as control

    2.Molecular weight markers are noted

    Thanks for sending along the blot image. Here are my thoughts:

    1) Where are the markers and marker labels?;

    2) Has any other antibody been used successfully with these samples?;

    3) It is mentioned 0.5mg protein loaded, Is ...

    Read More

    Thank you for submitting your completed questionnaire. I am sorry to hear this vial of ab5706 is giving you trouble.

    You mentioned including a western blot in your enquiry but unfortunately it did not make it through. If you could, please re ...

    Read More

    Thank you for confirming this information and for your help and cooperation with this case.

    As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order...

    Read More

    Thank you for your message and for kindly providing this further information.

    I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be plea...

    Read More

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will enable us to investigate this...

    Read More
    Mouse Cell (Pancreas)
    Blocking step
    Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% serum +

    Dr. Howard Crawford

    Verified customer

    Submitted Feb 21 2006


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