Product nameAnti-MMP7 antibody
See all MMP7 primary antibodies
DescriptionRabbit polyclonal to MMP7
SpecificityThis antibody specifically recognises MMP7, and does not cross react with MMP1, 2, 3, 8, 9, 10 or 16.
Tested applicationsSuitable for: WB, ELISA, IP, IHC-Fr, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide corresponding to C-Terminus of MMP7 (Human).
- PMA stimulated A431 whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab5706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 28 kDa.|
|ELISA||Use a concentration of 0.1 - 1 µg/ml.|
|IP||Use 3-5µg for 106 cells.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 18499699|
|ICC/IF||Use at an assay dependent concentration. PubMed: 24400123|
|IHC-P||Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionDegrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
Sequence similaritiesBelongs to the peptidase M10A family.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Matrilysin antibody
- Matrin antibody
- Matrix Metalloproteinase 7 antibody
Immunohistochemistry analysis of human prostate carcinoma stained with rabbit polyclonal anti-MMP-7 (ab5706).
All lanes : Anti-MMP7 antibody (ab5706)
Lane 1 : Mouse brain lysate
Lane 2 : Human MMP-7 over-expressing Hek293 cell lysate
ab5706 (2µg/ml) staining MMP7 in human kidney cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasm and nuclei within proximal convoluted tubules.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
This product has been referenced in:
- Tang H et al. MARCH5 overexpression contributes to tumor growth and metastasis and associates with poor survival in breast cancer. Cancer Manag Res 11:201-215 (2019). Read more (PubMed: 30636894) »
- Zhang XW et al. Long noncoding RNA AK089579 inhibits epithelial-to-mesenchymal transition of peritoneal mesothelial cells by competitively binding to microRNA-296-3p via DOK2 in peritoneal fibrosis. FASEB J 33:5112-5125 (2019). Read more (PubMed: 30652956) »