Overview

  • Product name
  • Description
    Rabbit polyclonal to MMP7
  • Host species
    Rabbit
  • Specificity
    This antibody specifically recognises MMP7, and does not cross react with MMP1, 2, 3, 8, 9, 10 or 16.
  • Tested applications
    Suitable for: WB, ELISA, IP, IHC-Fr, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to C-Terminus of MMP7 (Human).

  • Positive control
    • PMA stimulated A431 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab5706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IP
IHC-Fr
ICC/IF
IHC-P
  • Application notes
    ELISA: Use at a concentration of 0.1 - 1.0 µg/ml.
    IHC-Fr: Use at an assay dependent dilution (PMID 18499699).
    IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    IP: Use 3-5µg for 106 cells.
    WB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects a band of approximately 28 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
    • Sequence similarities
      Belongs to the peptidase M10A family.
    • Domain
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Matrilysin antibody
      • Matrin antibody
      • Matrix Metalloproteinase 7 antibody
      • Matrix metalloproteinase-7 antibody
      • MMP 7 antibody
      • MMP-7 antibody
      • MMP7 antibody
      • MMP7_HUMAN antibody
      • MPSL1 antibody
      • PUMP 1 antibody
      • Pump 1 protease antibody
      • Pump-1 protease antibody
      • PUMP1 antibody
      • Uterine matrilysin antibody
      • Uterine metalloproteinase antibody
      see all

    Images

    • Immunohistochemistry analysis of human prostate carcinoma stained with rabbit polyclonal anti-MMP-7 (ab5706).

    • All lanes : Anti-MMP7 antibody (ab5706)

      Lane 1 : Mouse brain lysate
      Lane 2 : Human MMP-7 over-expressing Hek293 cell lysate
    • ab5706 (2µg/ml) staining MMP7 in human kidney cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasm and nuclei within proximal convoluted tubules.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    References

    This product has been referenced in:
    • Diao B  et al. The role of galectin-3 in the tumorigenesis and progression of pituitary tumors. Oncol Lett 15:4919-4925 (2018). Read more (PubMed: 29545898) »
    • Soler A  et al. Elevated 20-HETE in metabolic syndrome regulates arterial stiffness and systolic hypertension via MMP12 activation. J Mol Cell Cardiol 117:88-99 (2018). WB . Read more (PubMed: 29428638) »

    See all 13 Publications for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (stomach cancer cell)
    Loading amount
    25 µg
    Specification
    stomach cancer cell
    Treatment
    10uM cannabinoid antagonist
    Gel Running Conditions
    Reduced Denaturing (7.5% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 08 2013

    Application
    Immunocytochemistry
    Sample
    Mouse Cell (Pancreas)
    Specification
    Pancreas
    Blocking step
    Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% serum +
    Username

    Dr. Howard Crawford

    Verified customer

    Submitted Feb 21 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up