• Product name
  • Description
    Rabbit polyclonal to MMP7
  • Host species
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Horse, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human MMP7.

    (Peptide available as ab95052.)

  • Positive control
    • This antibody gave a positive signal in A431 whole cell lysate and in the following human tissue lysates; Placenta; Liver; Kidney; Small Intestine.



Our Abpromise guarantee covers the use of ab85144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). Also detects a band at approximately 22 kDa.


  • Function
    Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
  • Sequence similarities
    Belongs to the peptidase M10A family.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Matrilysin antibody
    • Matrin antibody
    • Matrix Metalloproteinase 7 antibody
    • Matrix metalloproteinase-7 antibody
    • MMP 7 antibody
    • MMP-7 antibody
    • MMP7 antibody
    • MMP7_HUMAN antibody
    • MPSL1 antibody
    • PUMP 1 antibody
    • Pump 1 protease antibody
    • Pump-1 protease antibody
    • PUMP1 antibody
    • Uterine matrilysin antibody
    • Uterine metalloproteinase antibody
    see all


  • All lanes : Anti-MMP7 antibody (ab85144) at 1 µg/ml

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Human kidney tissue lysate - total protein (ab30203)
    Lane 5 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 22,29 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 200 kDa, 40 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 5 minutes

    MMP7 protein contains both a 17-amino acid signal sequence and an activation peptide (77 amino-acids) (Swissprot). If both were removed, the expected MW would be around 20-kDa. We hypothesise that the band at approximately 20-kDa, detected in A431 whole cell lysate, and in human liver, kidney and small intestine tissue lysates, is the cleaved form of MMP7. The band at approximately 29-kDa in human placenta tissue lysate is probably the full length protein.
  • IHC image of MMP7 staining in human pancreatic cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85144, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX


ab85144 has not yet been referenced specifically in any publications.

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