Overview

  • Product name

    Anti-MMP7 antibody [EPR1251(2)]
    See all MMP7 primary antibodies
  • Description

    Rabbit monoclonal [EPR1251(2)] to MMP7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MMP7 aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P09237

  • Positive control

    • Human prostate, BxPC-3 and Human colon cancer lysates. BxPC-3 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab176325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
    • Sequence similarities

      Belongs to the peptidase M10A family.
    • Domain

      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • Matrilysin antibody
      • Matrin antibody
      • Matrix Metalloproteinase 7 antibody
      • Matrix metalloproteinase-7 antibody
      • MMP 7 antibody
      • MMP-7 antibody
      • MMP7 antibody
      • MMP7_HUMAN antibody
      • MPSL1 antibody
      • PUMP 1 antibody
      • Pump 1 protease antibody
      • Pump-1 protease antibody
      • PUMP1 antibody
      • Uterine matrilysin antibody
      • Uterine metalloproteinase antibody
      see all

    Images

    • All lanes : Anti-MMP7 antibody [EPR1251(2)] (ab176325) at 1/1000 dilution

      Lane 1 : Human prostate lysate
      Lane 2 : BxPC-3 lysate
      Lane 3 : Human colon cancer lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 29 kDa

    • Flow cytometric analysis of permeabilized BxPC-3 cells labeling MMP7 using ab176325 at a 1/100 dilution (red) or a rabbit IgG control (green).

    References

    This product has been referenced in:

    • Xie Y  et al. MMP7 interacts with ARF in nucleus to potentiate tumor microenvironments for prostate cancer progression in vivo. Oncotarget 7:47609-47619 (2016). WB . Read more (PubMed: 27356744) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab176325.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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