Recombinant
RabMAb

Recombinant Anti-MMP7 antibody [EPR17888-71] (ab207299)

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Overview

  • Product name

    Anti-MMP7 antibody [EPR17888-71]
    See all MMP7 primary antibodies

  • Description

    Rabbit monoclonal [EPR17888-71] to MMP7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein within Human MMP7 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P09237

  • Positive control

    • WB: Human colon cancer lysate; A549 and BxPC-3 whole cell lysates. IHC-P: Human endometrial cancer and breast cancer tissues. ICC/IF: HT-29 and PC-3 cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab207299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/300.

Target

  • Function

    Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.

  • Sequence similarities

    Belongs to the peptidase M10A family.

  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Database links

    • Alternative names

      • Matrilysin antibody
      • Matrin antibody
      • Matrix Metalloproteinase 7 antibody
      • Matrix metalloproteinase-7 antibody
      • MMP 7 antibody
      • MMP-7 antibody
      • MMP7 antibody
      • MMP7_HUMAN antibody
      • MPSL1 antibody
      • PUMP 1 antibody
      • Pump 1 protease antibody
      • Pump-1 protease antibody
      • PUMP1 antibody
      • Uterine matrilysin antibody
      • Uterine metalloproteinase antibody
      see all

    Images

    • Western blot - Anti-MMP7 antibody [EPR17888-71] (ab207299)
      Western blot - Anti-MMP7 antibody [EPR17888-71] (ab207299)
      All lanes : Anti-MMP7 antibody [EPR17888-71] (ab207299) at 1/2000 dilution

      Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
      Lane 2 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 30 kDa
      Observed band size: 30 kDa


      Exposure time: 3 minutes


      5% NFDM/TBST: Blocking and dilution buffer.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-71] (ab207299)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-71] (ab207299)

      Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling MMP7 with ab207299 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Cytoplasm staining on Human endometrial cancer is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-MMP7 antibody [EPR17888-71] (ab207299)
      Immunocytochemistry/ Immunofluorescence - Anti-MMP7 antibody [EPR17888-71] (ab207299)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-3 (Human prostate adenocarcinoma cell line) cells labeling MMP7 with ab207299 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing cytoplasmic staining on PC-3 cell line.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

      The negative controls are as follows:-

      -ve control 1: ab207299 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-71] (ab207299)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-71] (ab207299)

      Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling MMP7 with ab207299 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Cytoplasm staining on cancer cells of breast cancer is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-MMP7 antibody [EPR17888-71] (ab207299)
      Western blot - Anti-MMP7 antibody [EPR17888-71] (ab207299)
      Anti-MMP7 antibody [EPR17888-71] (ab207299) at 1/1000 dilution + Human colon cancer lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 30 kDa
      Observed band size: 30 kDa


      Exposure time: 3 minutes


      5% NFDM/TBST: Blocking and dilution buffer.

    • Immunocytochemistry/ Immunofluorescence - Anti-MMP7 antibody [EPR17888-71] (ab207299)
      Immunocytochemistry/ Immunofluorescence - Anti-MMP7 antibody [EPR17888-71] (ab207299)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cell line) cells labeling MMP7 with ab207299 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing cytoplasmic staining on HT-29 cell line.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

      The negative controls are as follows:-

      -ve control 1: ab207299 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    References

    This product has been referenced in:

    • Xiang R  et al. Different effects of allergic rhinitis on nasal mucosa remodeling in chronic rhinosinusitis with and without nasal polyps. Eur Arch Otorhinolaryngol 276:115-130 (2019). Read more (PubMed: 30446828) »
    • Santoro R  et al. MEKK3 Sustains EMT and Stemness in Pancreatic Cancer by Regulating YAP and TAZ Transcriptional Activity. Anticancer Res 38:1937-1946 (2018). Read more (PubMed: 29599309) »
    See all 5 Publications for this product

    Publishing research using ab207299? Please let us know so that we can cite the reference in this datasheet.

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