Recombinant Anti-MMP7 antibody [EPR17888-71] (ab207299)

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Western blot - Anti-MMP7 antibody [EPR17888-71] (ab207299)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17888-71] to MMP7
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Carrier Free

Product name

Anti-MMP7 antibody [EPR17888-71]
See all MMP7 primary antibodies
Description

Rabbit monoclonal [EPR17888-71] to MMP7
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human colon cancer lysates; A549 and BxPC-3 whole cell lysates. IHC-P: Human endometrial cancer and breast cancer tissues. ICC/IF: HT-29 and PC-3 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17888-71
Isotype

IgG
Research areas
- Cancer
- Tumor biomarkers
- Enzymes
- MMPs
- Neuroscience
- Diseases

Alternative Versions
- Anti-MMP7 antibody [EPR17888-71] - Low endotoxin, Azide free (ab226005)
- Anti-MMP7 antibody [EPR17888-71] - BSA and Azide free (ab271977)
Assay kits
- MMP7 Inhibitor Screening Assay Kit (Fluorometric) (ab139445)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab207299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IHC-P		1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/300.

Notes
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/300.

Function

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
Sequence similarities

Belongs to the peptidase M10A family.
Domain

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization

Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accession P09237 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4316 Human
- Omim: 178990 Human
- SwissProt: P09237 Human
- Unigene: 2256 Human
Alternative names
- Matrilysin antibody
- Matrin antibody
- Matrix Metalloproteinase 7 antibody
- Matrix metalloproteinase-7 antibody
- MMP 7 antibody
- MMP-7 antibody
- MMP7 antibody
- MMP7_HUMAN antibody
- MPSL1 antibody
- PUMP 1 antibody
- Pump 1 protease antibody
- Pump-1 protease antibody
- PUMP1 antibody
- Uterine matrilysin antibody
- Uterine metalloproteinase antibody
see all

Western blot - Anti-MMP7 antibody [EPR17888-71] (ab207299)

All lanes : Anti-MMP7 antibody [EPR17888-71] (ab207299) at 1/2000 dilution

Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 30 kDa
Observed band size: 30 kDa

Exposure time: 3 minutes

5% NFDM/TBST: Blocking and dilution buffer.
Immunocytochemistry/ Immunofluorescence - Anti-MMP7 antibody [EPR17888-71] (ab207299)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-3 (Human prostate adenocarcinoma cell line) cells labeling MMP7 with ab207299 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on PC-3 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab207299 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-71] (ab207299)

Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling MMP7 with ab207299 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on Human endometrial cancer is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-71] (ab207299)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling MMP7 with ab207299 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on cancer cells of breast cancer is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-MMP7 antibody [EPR17888-71] (ab207299)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cell line) cells labeling MMP7 with ab207299 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on HT-29 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab207299 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Western blot - Anti-MMP7 antibody [EPR17888-71] (ab207299)

Anti-MMP7 antibody [EPR17888-71] (ab207299) at 1/1000 dilution + Human colon cancer lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 30 kDa
Observed band size: 30 kDa

Exposure time: 3 minutes

5% NFDM/TBST: Blocking and dilution buffer.
Anti-MMP7 antibody [EPR17888-71] (ab207299)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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Publishing research using ab207299? Please let us know so that we can cite the reference in this datasheet.

ab207299 has been referenced in 19 publications.

Wang X et al. lncRNA MEG3 inhibits pituitary tumor development by participating in cell proliferation, apoptosis and EMT processes. Oncol Rep 45:N/A (2021). PubMed: 33649837
Guo X et al. Circular RNA hsa_circ_0072309 inhibits the proliferation, invasion and migration of gastric cancer cells via inhibition of PI3K/AKT signaling by activating PPAR?/PTEN signaling. Mol Med Rep 23:N/A (2021). PubMed: 33760104
Sun CX et al. Demethylated miR-216a Regulates High Mobility Group Box 3 Promoting Growth of Esophageal Cancer Cells Through Wnt/ß-Catenin Pathway. Front Oncol 11:622073 (2021). PubMed: 33842327
Sai C et al. Macleayins A From Macleaya Promotes Cell Apoptosis Through Wnt/ß-Catenin Signaling Pathway and Inhibits Proliferation, Migration, and Invasion in Cervical Cancer HeLa Cells. Front Pharmacol 12:668348 (2021). PubMed: 34421589
Peng F et al. Downregulation of the Proton-Activated Cl- Channel TMEM206 Inhibits Malignant Properties of Human Osteosarcoma Cells. Oxid Med Cell Longev 2021:3672112 (2021). WB ; Human . PubMed: 34777684

View all Publications for this product

Customer reviews and Q&As

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Assay kits

Compatible Secondaries

Isotype control

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Domain

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (19)

Customer reviews and Q&As