MMP7 Inhibitor Screening Assay Kit (Fluorometric) (ab139445)

Overview

  • Product name

    MMP7 Inhibitor Screening Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Inhibitor compounds
  • Assay type

    Enzyme activity
  • Product overview

    Abcam MMP7 Inhibitor Screening Assay Kit (Fluorometric) (ab139445) is a complete assay system designed to screen MMP7 inhibitors using a quenched fluorogenic peptide: MMP Fluorogenic Substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 [Mca=(7-methoxycoumarin-4-yl)-acetyl; Dpa=N-3-(2,4-dinitrophenyl)-L-α-β-diaminopropionyl]. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format.

  • Notes

    This kit is useful to screen inhibitors of MMP7, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    96-well White Microplate 1/2 Volume 1 unit
    Fluorometric Assay Buffer 1 x 20ml
    MMP Calibration Standard 1 x 50µl
    MMP Fluorogenic Substrate 1 x 200µl
    MMP Inhibitor 1 x 50µl
    MMP7 Enzyme (Human, Recombinant) 1 x 33.3µl
  • Function

    Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
  • Sequence similarities

    Belongs to the peptidase M10A family.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names

    • Matrilysin
    • Matrin
    • Matrix metalloproteinase-7
    • MMP-7
    • MMP7
    • MMP7_HUMAN
    • Pump-1 protease
    • Uterine metalloproteinase
    see all

Images

Protocols

References

ab139445 has not yet been referenced specifically in any publications.

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