Overview

  • Product name
  • Description
    Rabbit polyclonal to MMP8
  • Host species
    Rabbit
  • Specificity
    MMP8 antibody detects endogenous levels of total MMP8 protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, Flow Cyt, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from human MMP8

  • Positive control
    • Human breast carcinoma tissue; extracts from NIH/3T3 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab53017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 21133852

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration. PubMed: 21133852
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • Function
    Can degrade fibrillar type I, II, and III collagens.
  • Tissue specificity
    Neutrophils.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localization
    Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLG 1 antibody
    • CLG1 antibody
    • Collagenase 1 antibody
    • Collagenase 1 neutrophil antibody
    • HNC antibody
    • Matrix metallopeptidase 8 (neutrophil collagenase) antibody
    • Matrix metalloprotease 8 antibody
    • Matrix metalloproteinase-8 antibody
    • MMP 8 antibody
    • MMP-8 antibody
    • Mmp8 antibody
    • MMP8_HUMAN antibody
    • Neutrophil collagenase antibody
    • PMNL CL antibody
    • PMNL collagenase antibody
    • PMNL-CL antibody
    • PMNLCL antibody
    see all

Images

  • All lanes : Anti-MMP8 antibody (ab53017) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells with no immunizing peptide
    Lane 2 : Extracts from NIH/3T3 cells with immunizing peptide

    Predicted band size: 53 kDa
    Observed band size: 53 kDa

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MMP8 antibody at 1/50 dilution. Left hand image; untreated tissue, Right hand image; tissue treated with the immunising peptide.
  • ICC/IF image of ab63017 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53017, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Mota R  et al. Increasing Cardiomyocyte Atrogin-1 Reduces Aging-Associated Fibrosis and Regulates Remodeling in Vivo. Am J Pathol 188:1676-1692 (2018). Read more (PubMed: 29758183) »
  • Spiegel A  et al. Neutrophils Suppress Intraluminal NK Cell-Mediated Tumor Cell Clearance and Enhance Extravasation of Disseminated Carcinoma Cells. Cancer Discov 6:630-49 (2016). Read more (PubMed: 27072748) »
See all 6 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer pH 6.0
Sample
Mouse Tissue sections (liver)
Specification
liver
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 04 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Non-Denaturing (Native)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 28 2014

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (human gut)
Specification
human gut
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Feb 14 2013

Application
Western blot
Sample
Rat Cell lysate - whole cell (rat vascular smooth muscle cells)
Loading amount
100000 cells
Specification
rat vascular smooth muscle cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Feb 05 2013

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.


I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused.

I was able to find the recommended protocol for use with CCR2 antibody ab25788. Please note the primary antibody concentration is 1:100. I hope this protocol helps you to achieve good staining with ab25788 anti-CCR2.



Protocol used by lab:

Blocking: 5% BSA for 2hr

Primary antibody: CCR2 antibody- 1:100 in 2.5% BSA (TBS/Tween 0.1%) 1hr at RT

Secondary antibody: Donkey anti-goat HRP - 1:8000 in 2.5% BSA (TBS/Tween 0.1%) 30 min at RT

Visualization: Amersham WB developer

As requested, I have issued a free of charge replacements for the anti-MMP8 and anti-MMP2 antibodies. I have also shipped a B cell lysate for positive control with ab25117 anti-SDF01 alpha. The order number for these is 1184512.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More
Application
Western blot
Sample
Human Tissue lysate - whole (human gut)
Loading amount
50 µg
Specification
human gut
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jul 03 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lymphoma)
Specification
lymphoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer (pH 6.0)
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 12 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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