Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Product nameMMP8 Inhibitor Screening Assay Kit (Fluorometric)
See all MMP8 kits
Sample typeInhibitor compounds
Assay typeEnzyme activity
Abcam MMP8 Inhibitor Screening Assay Kit (Fluorometric) (ab139453) is a complete assay system designed to sreen inhibitors of matrix metalloproteinase-8 (MMP8) using a quenched fluorogenic peptide: Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP8, a potential therapeutic target.
This kit is useful to screen inhibitors of MMP8, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 96-well White Microplate 1/2 Volume 1 unit Fluorometric Assay Buffer 1 x 20ml MMP Calibration Standard 1 x 50µl MMP Fluorogenic Substrate 1 x 200µl MMP Inhibitor 1 x 50µl MMP8 Enzyme (Human, Recombinant) 1 x 22µl
FunctionCan degrade fibrillar type I, II, and III collagens.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localizationCytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
- Information by UniProt
- CLG 1
- Collagenase 1
ab139453 has been referenced in 1 publication.
- Chen Z & Xue C G-Protein-Coupled Receptor 5 (LGR5) Overexpression Activates ß-Catenin Signaling in Breast Cancer Cells via Protein Kinase A. Med Sci Monit Basic Res 25:15-25 (2019). PubMed: 30662060