MMP8 Inhibitor Screening Assay Kit (Fluorometric) (ab139453)

Overview

  • Product name

    MMP8 Inhibitor Screening Assay Kit (Fluorometric)
    See all MMP8 kits
  • Detection method

    Fluorescent
  • Sample type

    Inhibitor compounds
  • Assay type

    Enzyme activity
  • Product overview

    Abcam MMP8 Inhibitor Screening Assay Kit (Fluorometric) (ab139453) is a complete assay system designed to sreen inhibitors of matrix metalloproteinase-8 (MMP8) using a quenched fluorogenic peptide: Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP8, a potential therapeutic target.

  • Notes

    This kit is useful to screen inhibitors of MMP8, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    96-well White Microplate 1/2 Volume 1 unit
    Fluorometric Assay Buffer 1 x 20ml
    MMP Calibration Standard 1 x 50µl
    MMP Fluorogenic Substrate 1 x 200µl
    MMP Inhibitor 1 x 50µl
    MMP8 Enzyme (Human, Recombinant) 1 x 22µl
  • Function

    Can degrade fibrillar type I, II, and III collagens.
  • Tissue specificity

    Neutrophils.
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localization

    Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
  • Information by UniProt
  • Alternative names

    • Matrix metalloproteinase-8
    • MMP-8
    • Mmp8
    • MMP8_HUMAN
    • Neutrophil collagenase
    • PMNL collagenase
    • PMNL-CL
    see all

Images

Protocols

References

This product has been referenced in:

  • Chen Z & Xue C G-Protein-Coupled Receptor 5 (LGR5) Overexpression Activates ß-Catenin Signaling in Breast Cancer Cells via Protein Kinase A. Med Sci Monit Basic Res 25:15-25 (2019). Read more (PubMed: 30662060) »
See 1 Publication for this product

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