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    mmp9-antibody-2c3-bsa-and-azide-free-ab270281.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP
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Anti-MMP9 antibody [2C3] - BSA and Azide free (ab270281)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [2C3] - BSA and Azide free (ab270281)

    Key features and details

    • Mouse monoclonal [2C3] to MMP9 - BSA and Azide free
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-MMP9 antibody [2C3] - BSA and Azide free
      See all MMP9 primary antibodies
    • Description

      Mouse monoclonal [2C3] to MMP9 - BSA and Azide free
    • Host species

      Mouse
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide corresponding to Human MMP9 aa 600-700.
      Database link: P14780

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • IHC-P: Human spleen tissue.
    • General notes

      ab270281 is a carrier free version of ab270246.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.2
      Constituent: PBS
    • Carrier free

      Yes
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      2C3
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Adhesion / ECM
      • Matrix Metalloproteinases
      • MMP
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Enzymes
      • MMP
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • ECM enzymes
      • MMPs
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • MMPs
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Metalloprotease
      • MMPs
      • Cancer
      • Tumor biomarkers
      • Enzymes
      • MMPs
      • Cardiovascular
      • Atherosclerosis
      • Thrombosis
      • Other
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    Associated products

    • Alternative Versions

      • Anti-MMP9 antibody [2C3] (ab270246)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab270281 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    Notes
    IHC-P
    Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Target

    • Function

      May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
      -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
    • Tissue specificity

      Produced by normal alveolar macrophages and granulocytes.
    • Involvement in disease

      Intervertebral disc disease
      Metaphyseal anadysplasia 2
    • Sequence similarities

      Belongs to the peptidase M10A family.
      Contains 3 fibronectin type-II domains.
      Contains 4 hemopexin repeats.
    • Domain

      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications

      Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
      N- and O-glycosylated.
    • Cellular localization

      Secreted, extracellular space, extracellular matrix.
    • Target information above from: UniProt accession P14780 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4318 Human
      • Omim: 120361 Human
      • SwissProt: P14780 Human
      • Unigene: 297413 Human
      • Alternative names

        • 82 kDa matrix metalloproteinase-9 antibody
        • 92 kDa gelatinase antibody
        • 92 kDa type IV collagenase antibody
        • CLG 4B antibody
        • CLG4B antibody
        • Collagenase Type 4 beta antibody
        • Collagenase type IV 92 KD antibody
        • EC 3.4.24.35 antibody
        • Gelatinase 92 KD antibody
        • Gelatinase B antibody
        • Gelatinase beta antibody
        • GelatinaseB antibody
        • GELB antibody
        • Macrophage gelatinase antibody
        • MANDP2 antibody
        • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
        • Matrix Metalloproteinase 9 antibody
        • MMP 9 antibody
        • MMP-9 antibody
        • MMP9 antibody
        • MMP9_HUMAN antibody
        • Type V collagenase antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [2C3] - BSA and Azide free (ab270281)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [2C3] - BSA and Azide free (ab270281)

        Formalin-fixed, paraffin-embedded human spleen tissue stained for MMP9 using ab270246 at 2 µg/ml in immunohistochemical analysis.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270246).

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab270281? Please let us know so that we can cite the reference in this datasheet.

      ab270281 has not yet been referenced specifically in any publications.

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