Anti-MMP9 antibody [56-2A4] (ab58803)

1:500 dilution of ab58803 detected MMP-9 on 10 µg of Human spleen lysates. Arrow indicates protein MMP-9 (~92 kDa). Lower bands indicate active forms of MMP-9

Immunocytochemical analysis of Human PANC-1 cells, labeling MMP9 with ab58803 (1/100). Cells were fixed in formaldehyde, permeabilized in 0.25% Triton-X100 and blocked in 1% BSA for 1 hour at 20°C. DAPI used to stain nuclei. 

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ab58803 staining MMP9 in murine rib sections by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.1% Triton. Samples were then blocked with 20% serum for 1 hour at 19°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa Fluor®488-conjugated donkey anti-mouse polyclonal (green)  (ab150105) was used as secondary antibody at a 1/200 dilution. Counterstain DAPI (blue).

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Immunohistochemical analysis of Rabbit spleen tissue section, labeling MMP9 with ab58803 (1/50 with PBS and 0.05% Tween-20 at 23°C for 1 hour). Heat mediated antigen retrieval, using 10mM Citrate pH6. 

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Immunofluorescence analysis of murine neural stem cells, staining MMP9 (red) with ab58803. Cells were fixed in paraformaldehyde and blocked and permeabilized with 0.1% Triton-X and 10% goat serum. Cells were incubated with primary antibody and an AlexaFluor®568-conjugated goat anti-mouse IgG (ab175473) was used as the secondary antibody.