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    mmp9-antibody-56-2a4-ab58803.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP
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Anti-MMP9 antibody [56-2A4] (ab58803)

  • Datasheet
  • SDS
Reviews (9) Submit a question References (85)

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Western blot - Anti-MMP9 antibody [56-2A4] (ab58803)

    Key features and details

    • Mouse monoclonal [56-2A4] to MMP9
    • Suitable for: IHC-P, IHC-Fr, ICC/IF, WB
    • Reacts with: Mouse, Rat, Rabbit, Guinea pig, Human
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-MMP9 antibody [56-2A4]
      See all MMP9 primary antibodies
    • Description

      Mouse monoclonal [56-2A4] to MMP9
    • Host species

      Mouse
    • Specificity

      Recognizes the ~92 kDa latent and the ~83 kDa active forms of MMP9. Does not react with MMP1, MMP2, MMP3 or MMP13. The antibody recognizes precursor (92 kDa) and active (83 kDa) forms of human and guinea pig MMP-9.
    • Tested applications

      Suitable for: IHC-P, IHC-Fr, ICC/IF, WBmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Rabbit, Guinea pig, Human
    • Immunogen

      Synthetic peptide corresponding to Human MMP9 aa 626-644.
      Sequence:

      LFSGRRLWRFDVKAQMVDP


      Database link: P14780
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Breast carcinoma tissue. Conditioned medium of human fibrosarcoma HT1080 cells The protein is secreted so you may need to concentrate the protein from cell culture media. Concentrate protein by TCA, acetone or ammonium sulphate precipitation. HL60 cell lysate, U937 cell lysate, HT1080 cell lysate, human spleen lysate
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.1% Sodium azide
      Constituents: 1.64% Sodium phosphate, 0.1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Is purified.
    • Clonality

      Monoclonal
    • Clone number

      56-2A4
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Adhesion / ECM
      • Matrix Metalloproteinases
      • MMP
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Enzymes
      • MMP
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • ECM enzymes
      • MMPs
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • MMPs
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Metalloprotease
      • MMPs
      • Cancer
      • Tumor biomarkers
      • Enzymes
      • MMPs
      • Cardiovascular
      • Atherosclerosis
      • Thrombosis
      • Other
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      • Neuroscience
      • Diseases
      • Neuroscience
      • Processes

    Associated products

    • Assay kits

      • MMP9 Inhibitor Screening Assay Kit (Fluorometric) (ab139449)
    • Compatible Secondaries

      • Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 568) (ab175473)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human MMP9 protein (Proenzyme) (ab82955)
    • Related Products

      • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab58803 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P (3)
    Use at an assay dependent concentration.
    IHC-Fr (3)
    Use at an assay dependent concentration.
    ICC/IF (1)
    Use at an assay dependent concentration. PubMed: 22496779
    WB (2)
    Use a concentration of 2 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 78 kDa).
    Notes
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF
    Use at an assay dependent concentration. PubMed: 22496779
    WB
    Use a concentration of 2 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 78 kDa).

    Target

    • Function

      May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
      -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
    • Tissue specificity

      Produced by normal alveolar macrophages and granulocytes.
    • Involvement in disease

      Intervertebral disc disease
      Metaphyseal anadysplasia 2
    • Sequence similarities

      Belongs to the peptidase M10A family.
      Contains 3 fibronectin type-II domains.
      Contains 4 hemopexin repeats.
    • Domain

      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications

      Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
      N- and O-glycosylated.
    • Cellular localization

      Secreted, extracellular space, extracellular matrix.
    • Target information above from: UniProt accession P14780 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4318 Human
      • Entrez Gene: 17395 Mouse
      • Entrez Gene: 81687 Rat
      • Omim: 120361 Human
      • SwissProt: P14780 Human
      • SwissProt: P41245 Mouse
      • SwissProt: P41246 Rabbit
      • SwissProt: P50282 Rat
      • Unigene: 297413 Human
      see all
    • Alternative names

      • 82 kDa matrix metalloproteinase-9 antibody
      • 92 kDa gelatinase antibody
      • 92 kDa type IV collagenase antibody
      • CLG 4B antibody
      • CLG4B antibody
      • Collagenase Type 4 beta antibody
      • Collagenase type IV 92 KD antibody
      • EC 3.4.24.35 antibody
      • Gelatinase 92 KD antibody
      • Gelatinase B antibody
      • Gelatinase beta antibody
      • GelatinaseB antibody
      • GELB antibody
      • Macrophage gelatinase antibody
      • MANDP2 antibody
      • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
      • Matrix Metalloproteinase 9 antibody
      • MMP 9 antibody
      • MMP-9 antibody
      • MMP9 antibody
      • MMP9_HUMAN antibody
      • Type V collagenase antibody
      see all

    Images

    • Western blot - Anti-MMP9 antibody [56-2A4] (ab58803)
      Western blot - Anti-MMP9 antibody [56-2A4] (ab58803)

      1:500 dilution of ab58803 detected MMP-9 on 10 µg of Human spleen lysates. Arrow indicates protein MMP-9 (~92 kDa). Lower bands indicate active forms of MMP-9

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (85)

    Publishing research using ab58803? Please let us know so that we can cite the reference in this datasheet.

    ab58803 has been referenced in 85 publications.

    • Leng Y  et al. Overexpression of microRNA-29b inhibits epithelial-mesenchymal transition and angiogenesis of colorectal cancer through the ETV4/ERK/EGFR axis. Cancer Cell Int 21:17 (2021). PubMed: 33407520
    • Shiu YT  et al. Natural Vascular Scaffolding Treatment Promotes Outward Remodeling During Arteriovenous Fistula Development in Rats. Front Bioeng Biotechnol 9:622617 (2021). PubMed: 33681159
    • Liu J  et al. Early Passive Leg Movement Prevents Against the Development of Heart Failure With Preserved Ejection Fraction in Rats. Front Cardiovasc Med 8:655009 (2021). PubMed: 33969019
    • Zhu L  et al. MicroRNA-363-3p inhibits tumor cell proliferation and invasion in oral squamous cell carcinoma cell lines by targeting SSFA2. Exp Ther Med 21:549 (2021). PubMed: 33850521
    • He Y  et al. Curcumin inhibits the proliferation and migration of vascular smooth muscle cells by targeting the chemerin / CMKLR1 / LCN2 axis. Aging (Albany NY) 13:13859-13875 (2021). PubMed: 34029211
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MMP9 antibody [56-2A4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Panc1 cell line)
    Permeabilization
    Yes - 0.25% Triton-X100
    Specification
    Panc1 cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 15 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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