Anti-MMP9 antibody [56-2A4] (ab58803)
![Western blot - Anti-MMP9 antibody [56-2A4] (ab58803)](https://www.abcam.com/ps/products/58/ab58803/Images/ab58803-218393-ab58803.jpg "Western blot - Anti-MMP9 antibody [56-2A4] (ab58803)")
Key features and details
- Mouse monoclonal [56-2A4] to MMP9
- Suitable for: IHC-P, IHC-Fr, ICC/IF, WB
- Reacts with: Mouse, Rat, Rabbit, Guinea pig, Human
- Isotype: IgG1
Overview
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Product name
Anti-MMP9 antibody [56-2A4]
See all MMP9 primary antibodies -
Description
Mouse monoclonal [56-2A4] to MMP9 -
Host species
Mouse -
Specificity
Recognizes the ~92 kDa latent and the ~83 kDa active forms of MMP9. Does not react with MMP1, MMP2, MMP3 or MMP13. The antibody recognizes precursor (92 kDa) and active (83 kDa) forms of human and guinea pig MMP-9. -
Tested applications
Suitable for: IHC-P, IHC-Fr, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Guinea pig, Human -
Immunogen
Synthetic peptide corresponding to Human MMP9 aa 626-644.
Sequence:LFSGRRLWRFDVKAQMVDP
Database link: P14780 -
Positive control
- Breast carcinoma tissue. Conditioned medium of human fibrosarcoma HT1080 cells The protein is secreted so you may need to concentrate the protein from cell culture media. Concentrate protein by TCA, acetone or ammonium sulphate precipitation. HL60 cell lysate, U937 cell lysate, HT1080 cell lysate, human spleen lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.1% Sodium azide
Constituents: 1.64% Sodium phosphate, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Is purified. -
Clonality
Monoclonal -
Clone number
56-2A4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (2) |
Use at an assay dependent concentration.
|
| IHC-Fr | (3) |
Use at an assay dependent concentration.
|
| ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 22496779
|
| WB | (1) |
Use a concentration of 2 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 78 kDa).
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 22496779 |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 78 kDa). |
Target
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Function
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. -
Tissue specificity
Produced by normal alveolar macrophages and granulocytes. -
Involvement in disease
Intervertebral disc disease
Metaphyseal anadysplasia 2 -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsProcessing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4318 Human
- Entrez Gene: 17395 Mouse
- Entrez Gene: 81687 Rat
- Omim: 120361 Human
- SwissProt: P14780 Human
- SwissProt: P41245 Mouse
- SwissProt: P41246 Rabbit
- SwissProt: P50282 Rat
see all -
Alternative names
- 82 kDa matrix metalloproteinase-9 antibody
- 92 kDa gelatinase antibody
- 92 kDa type IV collagenase antibody
see all
Images
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Western blot - Anti-MMP9 antibody [56-2A4] (ab58803)
1:500 dilution of ab58803 detected MMP-9 on 10 µg of Human spleen lysates. Arrow indicates protein MMP-9 (~92 kDa). Lower bands indicate active forms of MMP-9
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (85)
ab58803 has been referenced in 85 publications.
- Leng Y et al. Overexpression of microRNA-29b inhibits epithelial-mesenchymal transition and angiogenesis of colorectal cancer through the ETV4/ERK/EGFR axis. Cancer Cell Int 21:17 (2021). PubMed: 33407520
- Shiu YT et al. Natural Vascular Scaffolding Treatment Promotes Outward Remodeling During Arteriovenous Fistula Development in Rats. Front Bioeng Biotechnol 9:622617 (2021). PubMed: 33681159
- Liu J et al. Early Passive Leg Movement Prevents Against the Development of Heart Failure With Preserved Ejection Fraction in Rats. Front Cardiovasc Med 8:655009 (2021). PubMed: 33969019
- Zhu L et al. MicroRNA-363-3p inhibits tumor cell proliferation and invasion in oral squamous cell carcinoma cell lines by targeting SSFA2. Exp Ther Med 21:549 (2021). PubMed: 33850521
- He Y et al. Curcumin inhibits the proliferation and migration of vascular smooth muscle cells by targeting the chemerin / CMKLR1 / LCN2 axis. Aging (Albany NY) 13:13859-13875 (2021). PubMed: 34029211