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    mmp9-antibody-ab38898.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP
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Anti-MMP9 antibody (ab38898)

  • Datasheet
  • SDS
Reviews (46)Q&A (3)References (814)

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Western blot - Anti-MMP9 antibody (ab38898)

    Key features and details

    • Rabbit polyclonal to MMP9
    • Suitable for: WB
    • Reacts with: Mouse, Recombinant fragment
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-MMP9 antibody [RM1020] (ab283575)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-MMP9 antibody
      See all MMP9 primary antibodies
    • Description

      Rabbit polyclonal to MMP9
    • Host species

      Rabbit
    • Specificity

      The antibody binds to MMP-9, but does not cross react with the other MMP family members (MMP-1, MMP-2, MMP-3).

      In our hands, no signal or a very weak signal was observed in WB in native human samples (BLAST of full length mouse protein sequence showed 72% homology with the Human MMP9 sequence).

      Treatment increasing the expression of MMP-9 is recommended when using this antibody.

    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Recombinant fragment
    • Immunogen

      Full length protein corresponding to Mouse MMP9.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituent: 50% Glycerol
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Adhesion / ECM
      • Matrix Metalloproteinases
      • MMP
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Enzymes
      • MMP
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • ECM enzymes
      • MMPs
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • MMPs
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Metalloprotease
      • MMPs
      • Cancer
      • Tumor biomarkers
      • Enzymes
      • MMPs
      • Cardiovascular
      • Atherosclerosis
      • Thrombosis
      • Other
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      • Neuroscience
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      • Processes

    Associated products

    • Assay kits

      • MMP9 Inhibitor Screening Assay Kit (Fluorometric) (ab139449)
    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
      • Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HL-60 whole cell lysate (ab7914)
    • Recombinant Protein

      • Recombinant Human MMP9 protein (Proenzyme) (ab82955)
    • Related Products

      • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
      • Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab8069)
      • Anti-MMP2 antibody [6E3F8] (ab86607)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab38898 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (20)
    1/1000. Detects a band of approximately 92 kDa. When using colorimetric substrates such as BCIP/NBT use at a 1/5000 dilution (for chemiluminescent substrates). Detects a band of approximately 92-95 kDa (pro-form) and 82kDa (active form) (Human samples). Mouse MMP9 is larger, and on SDS PAGE gels runs about 102-105 kDa. Dilution optimised using Chromogenic detection.
    Notes
    WB
    1/1000. Detects a band of approximately 92 kDa. When using colorimetric substrates such as BCIP/NBT use at a 1/5000 dilution (for chemiluminescent substrates). Detects a band of approximately 92-95 kDa (pro-form) and 82kDa (active form) (Human samples). Mouse MMP9 is larger, and on SDS PAGE gels runs about 102-105 kDa. Dilution optimised using Chromogenic detection.

    Target

    • Function

      May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
      -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
    • Tissue specificity

      Produced by normal alveolar macrophages and granulocytes.
    • Involvement in disease

      Intervertebral disc disease
      Metaphyseal anadysplasia 2
    • Sequence similarities

      Belongs to the peptidase M10A family.
      Contains 3 fibronectin type-II domains.
      Contains 4 hemopexin repeats.
    • Domain

      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications

      Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
      N- and O-glycosylated.
    • Cellular localization

      Secreted, extracellular space, extracellular matrix.
    • Target information above from: UniProt accession P14780 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 17395 Mouse
      • SwissProt: P41245 Mouse
      • Alternative names

        • 82 kDa matrix metalloproteinase-9 antibody
        • 92 kDa gelatinase antibody
        • 92 kDa type IV collagenase antibody
        • CLG 4B antibody
        • CLG4B antibody
        • Collagenase Type 4 beta antibody
        • Collagenase type IV 92 KD antibody
        • EC 3.4.24.35 antibody
        • Gelatinase 92 KD antibody
        • Gelatinase B antibody
        • Gelatinase beta antibody
        • GelatinaseB antibody
        • GELB antibody
        • Macrophage gelatinase antibody
        • MANDP2 antibody
        • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
        • Matrix Metalloproteinase 9 antibody
        • MMP 9 antibody
        • MMP-9 antibody
        • MMP9 antibody
        • MMP9_HUMAN antibody
        • Type V collagenase antibody
        see all

      Images

      • Western blot - Anti-MMP9 antibody (ab38898)
        Western blot - Anti-MMP9 antibody (ab38898)
        Anti-MMP9 antibody (ab38898) + Human MMP9

        Observed band size: 88,92 kDa why is the actual band size different from the predicted?



        Ab38898 detects a band at 92 Kd (pro-form) and a band at 88 Kd (active form). Mouse MMP9 is slightly larger than human MMP9, and the antibody detects a band at about 105 Kd. It is recommend to concentrate samples by Gelatin-agarose affinity chromatography prior to Western blot usage. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples.

      Protocols

      • Recommended WB protocol

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (814)

      Publishing research using ab38898? Please let us know so that we can cite the reference in this datasheet.

      ab38898 has been referenced in 814 publications.

      • Mazzoni TS & Quagio-Grassiotto I Presence of the matrix metalloproteinases during the migration of the primordial germ cells in zebrafish gonadal ridge. Cell Tissue Res 383:707-722 (2021). PubMed: 32960354
      • Zhou Q  et al. Kinesin family member 3A stimulates cell proliferation, migration, and invasion of bladder cancer cells in vitro and in vivo. FEBS Open Bio 11:1487-1496 (2021). PubMed: 31774623
      • do Vale GT  et al. Nebivolol Prevents Up-Regulation of Nox2/NADPH Oxidase and Lipoperoxidation in the Early Stages of Ethanol-Induced Cardiac Toxicity. Cardiovasc Toxicol 21:224-235 (2021). PubMed: 33067693
      • Fu C  et al. CircTET1 Inhibits Retinoblastoma Progression via Targeting miR-492 and miR-494-3p through Wnt/ß-catenin Signaling Pathway. Curr Eye Res N/A:1-10 (2021). PubMed: 33108919
      • Xiao P  et al. LncRNA NEAT1 regulates chondrocyte proliferation and apoptosis via targeting miR-543/PLA2G4A axis. Hum Cell 34:60-75 (2021). PubMed: 33040229
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a question

      1-3 of 3 Q&A

      Question

      MMP-9 and fibronectin are closely associated - can product Anti-MMP9 antibody (ab38898) distinguish between MMP-9 and fibronectin?

      Read More

      Abcam community

      Verified customer

      Asked on Dec 10 2012

      Answer

      Product Anti-MMP9 antibody (ab38898)is specific to MMP-9, and will not cross-react with fibronectin..

      However, please note that MMP-9 binds to fibronectin, and fibronectin co-elutes with MMP-9 in affinity chromatography procedures. In plasma and tissue culture media samples, MMP-9 can be found associated with fibronectin.

      We have observed a complex of MMP-9 and fibronectin in SDS-PAGE applications, and it can be surprisingly difficult to dissociate the complex. Therefore, it is possible that product ab38898 is able to detect a complex of MMP-9 and fibronectin in certain applications; however, ab38898 is specific to MMP-9 alone.

      Read More

      Abcam Scientific Support

      Answered on Dec 10 2012

      Question

      Does product Anti-MMP9 antibody (ab38898) recognize both the pro-form and the active form of MMP-9?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 18 2012

      Answer

      Product Anti-MMP9 antibody (ab38898) is a polyclonal Ab raised against the full length mouse MMP-9 sequence, and will detect both the pro-form and the active form of the MMP-9 protein.

      Read More

      Abcam Scientific Support

      Answered on Apr 18 2012

      Question

      What is the anticipated WB band size for mouse and human MMP-9 probed with product Anti-MMP9 antibody (ab38898)?

      Read More

      Abcam community

      Verified customer

      Asked on Oct 27 2011

      Answer

      Product Anti-MMP9 antibody (ab38898) is a polyclonal Ab generated against full length mouse MMP-9; this Ab will detect both the pro-form and active form of mouse and human MMP-9.

      Mouse MMP-9 is slightly larger than human MMP-9. Human MMP-9 has a calculated MW of approximately 78.5 kDa. However, human MMP-9 is glycosylated at several amino acid residues and due to this glycosylation runs at an observed MW of approximately 92 kDa on non-reducing SDS-PAGE gels, and an observed MW of approximately 95 kDa on reducing gels.

      Mouse MMP-9 has a calculated MW of approximately 80.5 kDa, and is more heavily glycosylated than its human ortholog. Mouse MMP-9 therefore runs at an observed MW of approximately 105 kDa on reducing SDS-PAGE gels.

      In both mouse and human, MMP-9 is cleaved into a variety of forms: first by loss of the signal sequence, then by the loss of the propeptide domain, followed by a succession of cleavage events at the carboxyterminal hemopexin-like domain. These cleavage events can generate an apparent MW ladder of MMP-9 forms, running from the latent enzyme (approximately 100 kDa) to the smallest cleaved fragments in the 30 kDa range.

      Read More

      Abcam Scientific Support

      Answered on Oct 27 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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