Anti-MMP9 antibody (ab38898)

Product Anti-MMP9 antibody (ab38898)is specific to MMP-9, and will not cross-react with fibronectin..

However, please note that MMP-9 binds to fibronectin, and fibronectin co-elutes with MMP-9 in affinity chromatography procedures. In plasma and tissue culture media samples, MMP-9 can be found associated with fibronectin.

We have observed a complex of MMP-9 and fibronectin in SDS-PAGE applications, and it can be surprisingly difficult to dissociate the complex. Therefore, it is possible that product ab38898 is able to detect a complex of MMP-9 and fibronectin in certain applications; however, ab38898 is specific to MMP-9 alone.

Product Anti-MMP9 antibody (ab38898) is a polyclonal Ab raised against the full length mouse MMP-9 sequence, and will detect both the pro-form and the active form of the MMP-9 protein.

Product Anti-MMP9 antibody (ab38898) is a polyclonal Ab generated against full length mouse MMP-9; this Ab will detect both the pro-form and active form of mouse and human MMP-9.

Mouse MMP-9 is slightly larger than human MMP-9. Human MMP-9 has a calculated MW of approximately 78.5 kDa. However, human MMP-9 is glycosylated at several amino acid residues and due to this glycosylation runs at an observed MW of approximately 92 kDa on non-reducing SDS-PAGE gels, and an observed MW of approximately 95 kDa on reducing gels.

Mouse MMP-9 has a calculated MW of approximately 80.5 kDa, and is more heavily glycosylated than its human ortholog. Mouse MMP-9 therefore runs at an observed MW of approximately 105 kDa on reducing SDS-PAGE gels.

In both mouse and human, MMP-9 is cleaved into a variety of forms: first by loss of the signal sequence, then by the loss of the propeptide domain, followed by a succession of cleavage events at the carboxyterminal hemopexin-like domain. These cleavage events can generate an apparent MW ladder of MMP-9 forms, running from the latent enzyme (approximately 100 kDa) to the smallest cleaved fragments in the 30 kDa range.