Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Hippocampal astrocyte culture)
Permeabilization
Yes - Methanol
Specification
Hippocampal astrocyte culture
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Alexa fluor conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Additional data
Additional Notes
Hippocampal astrocyte 24 h after seeding. Red Fluorescence denotes MMP-9 expression in astrocytes in the untreated condition. Hoechst #33258 stained the nuclei (blue).
PubMed ID
27431094: View
Abcam user community
Verified customer
Submitted Apr 03 2017