Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-15%)
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 8°C · Diluent: 2.5% milk in PBS-Tween20
Dilution
1/300
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488-conjugated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Detection
Detection method
Fluorescence detection
Bands
Specific: 85 kDa
Exposure
3 minute(s) and 0 second(s)
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Abcam user community
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Submitted Nov 03 2014