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    mmp9-antibody-ep1255y-ab137867.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP
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RecombinantRabMAb

Recombinant Anti-MMP9 antibody [EP1255Y] (ab137867)

  • Datasheet
  • SDS
Reviews (6) Submit a question References (82)

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Western blot - Anti-MMP9 antibody [EP1255Y] (ab137867)
  • Western blot - Anti-MMP9 antibody [EP1255Y] (ab137867)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)
  • Anti-MMP9 antibody [EP1255Y] (ab137867)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1255Y] to MMP9
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free HRP

You may also be interested in

Primary
Product image
HRP Anti-MMP9 antibody [EP1255Y] (ab194316)
Protein
Product image
Native Mouse MMP9 protein (ab39309)
Protein
Product image
Recombinant Human MMP9 protein (Proenzyme) (ab82955)

View more associated products

Overview

  • Product name

    Anti-MMP9 antibody [EP1255Y]
    See all MMP9 primary antibodies
  • Description

    Rabbit monoclonal [EP1255Y] to MMP9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MMP9 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: U937, A549 (Human lung carcinoma epithelial cell) serum starved overnight, then treated with 80nM TPA for 24 hours whole cell lysate and MDA-MB-231 (Human breast adenocarcinoma epithelial cell) serum starved overnight, then treated with 200nM TPA for 24 hours whole cell lysate IHC-P: Human tonsil and lymphoma tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1255Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Matrix Metalloproteinases
    • MMP
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • MMP
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • ECM enzymes
    • MMPs
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MMPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • MMPs
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • MMPs
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other
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    • Neuroscience
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    • Neuroscience
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Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-MMP9 antibody [EP1255Y] (ab194314)
    • HRP Anti-MMP9 antibody [EP1255Y] (ab194316)
    • Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)
    • Anti-MMP9 antibody [EP1255Y] - BSA and Azide free (ab271910)
  • Assay kits

    • MMP9 Inhibitor Screening Assay Kit (Fluorometric) (ab139449)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human MMP9 protein (Proenzyme) (ab82955)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (5)
1/1000. Predicted molecular weight: 78 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/800.

Notes
WB
1/1000. Predicted molecular weight: 78 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/800.

Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • Tissue specificity

    Produced by normal alveolar macrophages and granulocytes.
  • Involvement in disease

    Intervertebral disc disease
    Metaphyseal anadysplasia 2
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix.
  • Target information above from: UniProt accession P14780 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4318 Human
    • Omim: 120361 Human
    • SwissProt: P14780 Human
    • Unigene: 297413 Human
    • Alternative names

      • 82 kDa matrix metalloproteinase-9 antibody
      • 92 kDa gelatinase antibody
      • 92 kDa type IV collagenase antibody
      • CLG 4B antibody
      • CLG4B antibody
      • Collagenase Type 4 beta antibody
      • Collagenase type IV 92 KD antibody
      • EC 3.4.24.35 antibody
      • Gelatinase 92 KD antibody
      • Gelatinase B antibody
      • Gelatinase beta antibody
      • GelatinaseB antibody
      • GELB antibody
      • Macrophage gelatinase antibody
      • MANDP2 antibody
      • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
      • Matrix Metalloproteinase 9 antibody
      • MMP 9 antibody
      • MMP-9 antibody
      • MMP9 antibody
      • MMP9_HUMAN antibody
      • Type V collagenase antibody
      see all

    Images

    • Western blot - Anti-MMP9 antibody [EP1255Y] (ab137867)
      Western blot - Anti-MMP9 antibody [EP1255Y] (ab137867)
      All lanes : Anti-MMP9 antibody [EP1255Y] (ab137867) at 1/1000 dilution

      Lane 1 : LoVo (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
      Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
      Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 5 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
      Lane 6 : A549 (Human lung carcinoma epithelial cell) serum starved overnight whole cell lysate
      Lane 7 : A549 (Human lung carcinoma epithelial cell) serum starved overnight, then treated with 80nM TPA for 24 hours whole cell lysate
      Lane 8 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) serum starved overnight whole cell lysate
      Lane 9 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) serum starved overnight, then treated with 200nM TPA for 24 hours whole cell lysate
      Lane 10 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 78 kDa
      Observed band size: 84 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      Blocking buffer and concentration 5% NFDM/TBST
      Diluting buffer and concentration 5% NFDM/TBST

      The expression of MMP-9 can be stimulated by various agents, such as inflammatory cytokine, growth factor, and 12-O-tetradecanoylphorbol-13-acetate (TPA) (PMID:21047770, 28969043).

      Treatment increasing the expression of MMP-9 is recommended when using this antibody.

    • Western blot - Anti-MMP9 antibody [EP1255Y] (ab137867)
      Western blot - Anti-MMP9 antibody [EP1255Y] (ab137867)
      Anti-MMP9 antibody [EP1255Y] (ab137867) at 1/1000 dilution (unpurified) + U937 cell lysate at 10 µg

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 78 kDa
      Observed band size: 78 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling MMP9 with unpurified ab137867 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling MMP9 with purified ab137867 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tissue labelling MMP9 with unpurified ab137867 at 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-MMP9 antibody [EP1255Y] (ab137867)
      Anti-MMP9 antibody [EP1255Y] (ab137867)

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (82)

    Publishing research using ab137867? Please let us know so that we can cite the reference in this datasheet.

    ab137867 has been referenced in 82 publications.

    • Cui S  et al. LncRNA EWSAT1 Regulates the Tumorigenesis of NSCLC as a ceRNA by Modulating miR-330-5p/ITGA5 Axis. Biochem Genet N/A:N/A (2021). PubMed: 33928467
    • Ye D  et al. Silencing PRSS1 suppresses the growth and proliferation of gastric carcinoma cells via the ERK pathway. Int J Biol Sci 17:957-971 (2021). PubMed: 33867821
    • Yin P  et al. MMP-9 Knockdown Inhibits Oral Squamous Cell Carcinoma Lymph Node Metastasis in the Nude Mouse Tongue-Xenografted Model through the RhoC/Src Pathway. Anal Cell Pathol (Amst) 2021:6683391 (2021). PubMed: 33828938
    • Orset C  et al. Combination treatment with U0126 and rt-PA prevents adverse effects of the delayed rt-PA treatment after acute ischemic stroke. Sci Rep 11:11993 (2021). PubMed: 34099834
    • Ye D  et al. LMP1 Up-regulates Calreticulin to Induce Epithelial-mesenchymal Transition via TGF-ß/Smad3/NRP1 Pathway in Nasopharyngeal Carcinoma Cells. J Cancer 11:1257-1269 (2020). PubMed: 31956372
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-6 of 6 Abreviews

    Western blot abreview for Anti-MMP9 antibody [EP1255Y]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (melanoma cells)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    10 µg
    Treatment
    canine recombinant IL-1β
    Specification
    melanoma cells
    Blocking step
    blockace as blocking agent for 50 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 08 2022

    Western blot abreview for Anti-MMP9 antibody [EP1255Y]

    Inconclusive
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Colon)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    25 µg
    Specification
    Colon
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Lewins Walter

    Verified customer

    Submitted Aug 12 2016

    Western blot abreview for Anti-MMP9 antibody [EP1255Y]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (whole embryo lysate)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    40 µg
    Specification
    whole embryo lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mrs. Ira Rostovsky

    Verified customer

    Submitted Mar 02 2016

    Western blot abreview for Anti-MMP9 antibody [EP1255Y]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Trophoblast)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    Trophoblast
    Blocking step
    4% block from PE as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 14 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-MMP9 antibody [EP1255Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Dog Tissue sections (Atria)
    Specification
    Atria
    Permeabilization
    Yes - 0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Juan Estrada

    Verified customer

    Submitted Jul 28 2014

    Western blot abreview for Anti-MMP9 antibody [EP1255Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    8 µg
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (4% stacking, 7.5% resolving)
    Sample
    Dog Tissue lysate - whole (atria)
    Specification
    atria
    Blocking step
    KPL Signa Lock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 02 2014

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