Anti-MMP9 antibody [EP1255Y] (ab137867)
- Datasheet
- Specific References (25)
- Protocols
Overview
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Product nameAnti-MMP9 antibody [EP1255Y]
See all MMP9 primary antibodies -
DescriptionRabbit monoclonal [EP1255Y] to MMP9
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Fr, WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human MMP9 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: U937 cell lysate. IHC-P: Human tonsil and lymphoma tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEP1255Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab137867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | 1/1000. Predicted molecular weight: 78 kDa. | |
| IHC-P | 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/800. |
Target
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FunctionMay play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. -
Tissue specificityProduced by normal alveolar macrophages and granulocytes.
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Involvement in diseaseIntervertebral disc disease
Metaphyseal anadysplasia 2 -
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats. -
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
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Post-translational
modificationsProcessing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated. -
Cellular localizationSecreted, extracellular space, extracellular matrix.
- Information by UniProt
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Database links
- Entrez Gene: 4318 Human
- Omim: 120361 Human
- SwissProt: P14780 Human
- Unigene: 297413 Human
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Alternative names
- 82 kDa matrix metalloproteinase-9 antibody
- 92 kDa gelatinase antibody
- 92 kDa type IV collagenase antibody
see all
Images
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![Western blot - Anti-MMP9 antibody [EP1255Y] (ab137867)](https://www.abcam.com/ps/products/137/ab137867/Images/ab137867-238898-ab137867upwb.jpg)
Western blot - Anti-MMP9 antibody [EP1255Y] (ab137867)Anti-MMP9 antibody [EP1255Y] (ab137867) at 1/1000 dilution (unpurified) + U937 cell lysate at 10 µg
Secondary
Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)](https://www.abcam.com/ps/products/137/ab137867/Images/ab137867-238900-ab137867upihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling MMP9 with unpurified ab137867 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)](https://www.abcam.com/ps/products/137/ab137867/Images/ab137867-238901-ab137867pihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling MMP9 with purified ab137867 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)](https://www.abcam.com/ps/products/137/ab137867/Images/MMP9-Primary-antibodies-ab137867-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tissue labelling MMP9 with unpurified ab137867 at 1/100.
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![Immunohistochemistry (Frozen sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)](https://www.abcam.com/ps/products/137/ab137867/Images/ab137867-230541-ab137867ihcf.jpg)
Immunohistochemistry (Frozen sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)This image is courtesy of an Abreview submitted by Juan EstradaUnpurified ab137867 staining MMP9 in 12µm dog atria tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde, permeabilized and blocked with PBS + 0.3% Triton X-100 + 10mg/mL BSA + 5% normal goat serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 10mg/mL BSA + 5% normal goat serum) for 6 hours at 25°C. A Cy2®-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
References
This product has been referenced in:
- Gao Y & Ge W The histone methyltransferase DOT1L inhibits osteoclastogenesis and protects against osteoporosis. Cell Death Dis 9:33 (2018). Read more (PubMed: 29348610) »
- Min JJ et al. Effects of Pravastatin on Type 1 Diabetic Rat Heart with or without Blood Glycemic Control. J Diabetes Res 2018:1067853 (2018). Read more (PubMed: 29682576) »
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