Name: Anti-MMP9 antibody [EP1255Y]
Brand: RabMAb
SKU: ab137867
Rating: 4 (5 reviews)

Add a compatible secondary antibody

Primary

Secondary

This product Rabbit Anti-MMP9 antibody [EP1255Y] (ab137867)
IHC-Fr, WB, IHC-P

Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC-P, WB, ELISA, IP

The primary selected is not available to customers in this country.

Sorry, we can't display this right now.
Please contact us to place your order, or try again later.

Product name

Anti-MMP9 antibody [EP1255Y]
See all MMP9 primary antibodies
Description

Rabbit monoclonal [EP1255Y] to MMP9
Host species

Rabbit
Tested applications

Suitable for: IHC-Fr, WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human MMP9 (C terminal). The exact sequence is proprietary.
Positive control
- WB: U937, A549 (Human lung carcinoma epithelial cell) serum starved overnight, then treated with 80nM TPA for 24 hours whole cell lysate and MDA-MB-231 (Human breast adenocarcinoma epithelial cell) serum starved overnight, then treated with 200nM TPA for 24 hours whole cell lysate IHC-P: Human tonsil and lymphoma tissues.
General notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1255Y
Isotype

IgG
Research areas
- Cancer
- Tumor biomarkers
- Enzymes
- MMPs

Alternative Versions
Assay kits
- MMP9 Inhibitor Screening Assay Kit (Fluorometric) (ab139449)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human MMP9 protein (Proenzyme) (ab82955)

Our Abpromise guarantee covers the use of ab137867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

IHC-Fr

Use at an assay dependent concentration.

1/1000. Predicted molecular weight: 78 kDa.

IHC-P

1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/800.

Application notes

Is unsuitable for Flow Cyt,ICC/IF or IP.

Function

May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Tissue specificity

Produced by normal alveolar macrophages and granulocytes.
Involvement in disease

Intervertebral disc disease
Metaphyseal anadysplasia 2
Sequence similarities

Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats.
Domain

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications

Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated.
Cellular localization

Secreted, extracellular space, extracellular matrix.
Target information above from: UniProt accession P14780 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4318 Human
- Omim: 120361 Human
- SwissProt: P14780 Human
- Unigene: 297413 Human
Alternative names
- 82 kDa matrix metalloproteinase-9 antibody
- 92 kDa gelatinase antibody
- 92 kDa type IV collagenase antibody
- CLG 4B antibody
- CLG4B antibody
- Collagenase Type 4 beta antibody
- Collagenase type IV 92 KD antibody
- EC 3.4.24.35 antibody
- Gelatinase 92 KD antibody
- Gelatinase B antibody
- Gelatinase beta antibody
- GelatinaseB antibody
- GELB antibody
- Macrophage gelatinase antibody
- MANDP2 antibody
- Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
- Matrix Metalloproteinase 9 antibody
- MMP 9 antibody
- MMP-9 antibody
- MMP9 antibody
- MMP9_HUMAN antibody
- Type V collagenase antibody
see all

Western blot - Anti-MMP9 antibody [EP1255Y] (ab137867)

All lanes : Anti-MMP9 antibody [EP1255Y] (ab137867) at 1/1000 dilution

Lane 1 : LoVo (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 6 : A549 (Human lung carcinoma epithelial cell) serum starved overnight whole cell lysate
Lane 7 : A549 (Human lung carcinoma epithelial cell) serum starved overnight, then treated with 80nM TPA for 24 hours whole cell lysate
Lane 8 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) serum starved overnight whole cell lysate
Lane 9 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) serum starved overnight, then treated with 200nM TPA for 24 hours whole cell lysate
Lane 10 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 78 kDa
Observed band size: 84 kDa
why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM/TBST

The expression of MMP-9 can be stimulated by various agents, such as inflammatory cytokine, growth factor, and 12-O-tetradecanoylphorbol-13-acetate (TPA) (PMID:21047770, 28969043).

Treatment increasing the expression of MMP-9 is recommended when using this antibody.
Western blot - Anti-MMP9 antibody [EP1255Y] (ab137867)

Anti-MMP9 antibody [EP1255Y] (ab137867) at 1/1000 dilution (unpurified) + U937 cell lysate at 10 µg

Secondary
Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 78 kDa
Observed band size: 78 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling MMP9 with unpurified ab137867 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling MMP9 with purified ab137867 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tissue labelling MMP9 with unpurified ab137867 at 1/100.
Immunohistochemistry (Frozen sections) - Anti-MMP9 antibody [EP1255Y] (ab137867)This image is courtesy of an Abreview submitted by Juan Estrada

Unpurified ab137867 staining MMP9 in 12µm dog atria tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde, permeabilized and blocked with PBS + 0.3% Triton X-100 + 10mg/mL BSA + 5% normal goat serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 10mg/mL BSA + 5% normal goat serum) for 6 hours at 25°C. A Cy2^®-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
See Abreview

Immunohistochemistry protocols
Western blot protocols

This product has been referenced in:

Cui C et al. Znhit1 inhibits breast cancer by up-regulating PTEN to deactivate the PI3K/Akt/mTOR pathway. Life Sci 224:204-211 (2019). Read more (PubMed: 30928405) »
Li P et al. Long noncoding RNA H19 participates in metformin-mediated inhibition of gastric cancer cell invasion. J Cell Physiol 234:4515-4527 (2019). Read more (PubMed: 30192003) »

See all 36 Publications for this product

Publishing research using ab137867? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review

1-5 of 5 Abreviews

Application

Western blot

Sample

Rat Tissue lysate - whole (Colon)

Gel Running Conditions

Non-reduced Denaturing

Loading amount

25 µg

Specification

Colon

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Lewins Walter

Verified customer

Submitted Aug 12 2016

Application

Western blot

Sample

Mouse Tissue lysate - whole (whole embryo lysate)

Gel Running Conditions

Reduced Denaturing (10%)

Loading amount

40 µg

Specification

whole embryo lysate

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Mrs. Ira Rostovsky

Verified customer

Submitted Mar 02 2016

Application

Western blot

Sample

Human Cell lysate - whole cell (Trophoblast)

Gel Running Conditions

Reduced Denaturing

Loading amount

10 µg

Specification

Trophoblast

Blocking step

4% block from PE as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 14 2015

Application

Immunohistochemistry (Frozen sections)

Blocking step

0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Sample

Dog Tissue sections (Atria)

Specification

Atria

Permeabilization

Yes - 0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS

Fixative

Paraformaldehyde

Dr. Juan Estrada

Verified customer

Submitted Jul 28 2014

Application

Western blot

Loading amount

8 µg

Gel Running Conditions

Reduced Non-Denaturing (Native) (4% stacking, 7.5% resolving)

Sample

Dog Tissue lysate - whole (atria)

Specification

atria

Blocking step

KPL Signa Lock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 02 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Location selector

Your location is currently set to:

Custom solutions & partnerships

Recombinant Anti-MMP9 antibody [EP1255Y] (ab137867)

Overview

Properties

Associated products

Applications

Target

Images

Protocols

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Western blot abreview for Anti-MMP9 antibody [EP1255Y]

Western blot abreview for Anti-MMP9 antibody [EP1255Y]

Western blot abreview for Anti-MMP9 antibody [EP1255Y]

Immunohistochemistry (Frozen sections) abreview for Anti-MMP9 antibody [EP1255Y]

Western blot abreview for Anti-MMP9 antibody [EP1255Y]