Western blot abreview for Anti-MMP9 antibody [EP1255Y]

Excellent
Abreviews
Application
Western blot
Loading amount
8 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (4% stacking, 7.5% resolving)
Sample
Dog Tissue lysate - whole (atria)
Specification
atria
Blocking step
KPL Signa Lock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Other product details

Incubation time
4 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: KPL Signa Lock
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Rabbit IgG(whole molecule), F(ab')2 fragment,
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL Pico
Bands
Specific: 92, 82 kDa
Exposure
8 minute(s) and 0 second(s)
Positive control
white blood cells from canine

Additional data

Additional Notes
RIPA lysates from canine atrial tissue and white blood cells

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Submitted May 02 2014

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