Immunohistochemistry (Frozen sections) abreview for Anti-MMP9 antibody [EP1255Y]

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Blocking step
0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Dog Tissue sections (Atria)
Specification
Atria
Permeabilization
Yes - 0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS
Fixative
Paraformaldehyde

Other product details

Incubation time
6 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 10mg/mL BSA, 5% Normal Goat Serum, in PB
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Jackson Immuno
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy2 ­
Dilution
1/200

Additional data

Additional Notes
4% PFA fixed tissue, 12um section, blocking and permeabilization combined as 1 step. 10mg/mL BSA, 5% Normal Goat Serum in PBS used as antibody diluent and as wash buffer. Image captured on epifluorescence microscope. Composite image, green channel Cy2 (CD34), red channel autofluorescence.

Dr. Juan Estrada

Verified customer

Submitted Jul 28 2014

Sign up