Western blot abreview for Anti-MMP9 antibody [EP1255Y]

Poor
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Trophoblast)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Trophoblast
Blocking step
4% block from PE as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: block buffer/TBST
Dilution
1/2000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Vector
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 90 kDa Non-specific: 55, 45 kDa

Additional data

Abcam response
Thank you for contacting us. You may want to adjust the dilution to 1/500 to see if this gives you the expetced band, although I can see some background bands exist at this dilution. Please note our Abpromise guarantee does protect the antibody for the tested species and applications for 12 months from purchase, and we can provide a replacement antibody, credit or refund if the antibody is not working as it should.

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Verified customer

Submitted Jul 14 2015

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