Thank you for contacting us. You may want to adjust the dilution to 1/500 to see if this gives you the expetced band, although I can see some background bands exist at this dilution. Please note our Abpromise guarantee does protect the antibody for the tested species and applications for 12 months from purchase, and we can provide a replacement antibody, credit or refund if the antibody is not working as it should.
Abcam user community
Submitted Jul 14 2015
Get resources and offers direct to your inboxSign up