Western blot abreview for Anti-MMP9 antibody [EP1255Y]

Average
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (whole embryo lysate)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
whole embryo lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/800

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: AffiPure Goat Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 100, 92, 82 kDa Non-specific: 55 kDa

Additional data

Additional Notes
Expected pro-active protein size in mouse suppose to be either 105, 97, or 91 kDa, a bit larger than the human MMP 9, depending on study.. Active forms are multiple sizes depending on study, 79, 78, 81, 86 kDa. Samples 1-6,8-9, are from embryonic mice, which normally expected as an Pro-and active MMP 9. Sample No'10 is from U937 cells extract. Compare to cells extract (10), it is looked that the antibody corresponded to the Mouse's MMP 9, but we also got a lot of nonspecific bands.

Mrs. Ira Rostovsky

Verified customer

Submitted Mar 02 2016

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