Western blot abreview for Anti-MMP9 antibody [EP1255Y]

Inconclusive
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Colon)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
25 µg
Specification
Colon
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5 % nonfat dry milk

Secondary antibody

Name
Non-Abcam antibody was used: Anti-mouse
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 66 kDa Non-specific: 55 kDa

Additional data

Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. We encourage all our customers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.

Lewins Walter

Verified customer

Submitted Aug 12 2016

Sign up