Recombinant Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)
- Datasheet
- References (8)
- Protocols
Overview
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Product nameAnti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free
See all MMP9 primary antibodies -
DescriptionRabbit monoclonal [EP1255Y] to MMP9 - Low endotoxin, Azide free
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivityReacts with: Dog, Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MMP9 (C terminal).
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Positive control
- WB: U937 cell lysate. IHC-P: Human tonsil and lymphoma tissues.
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General notes
The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferConstituent: PBS
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Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEP1255Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab219372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 78 kDa. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. |
Target
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FunctionMay play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. -
Tissue specificityProduced by normal alveolar macrophages and granulocytes.
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Involvement in diseaseIntervertebral disc disease
Metaphyseal anadysplasia 2 -
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats. -
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
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Post-translational
modificationsProcessing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated. -
Cellular localizationSecreted, extracellular space, extracellular matrix.
- Information by UniProt
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Database links
- Entrez Gene: 403885 Dog
- Entrez Gene: 4318 Human
- Omim: 120361 Human
- SwissProt: O18733 Dog
- SwissProt: P14780 Human
- Unigene: 297413 Human
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Alternative names
- 82 kDa matrix metalloproteinase-9 antibody
- 92 kDa gelatinase antibody
- 92 kDa type IV collagenase antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)](https://www.abcam.com/ps/products/219/ab219372/Images/ab219372-326723-anti-mmp9-antibody-ep1255y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling MMP9 with unpurified ab137867 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137867).
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![Immunohistochemistry (Frozen sections) - Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)](https://www.abcam.com/ps/products/219/ab219372/Images/ab219372-326722-anti-mmp9-antibody-ep1255y-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)This image is courtesy of an Abreview submitted by Juan Estrada.Unpurified ab137867 staining MMP9 in 12µm dog atria tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde, permeabilized and blocked with PBS + 0.3% Triton X-100 + 10mg/mL BSA + 5% normal goat serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 10mg/mL BSA + 5% normal goat serum) for 6 hours at 25°C. A Cy2®-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137867).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)](https://www.abcam.com/ps/products/219/ab219372/Images/ab219372-2-MMP9Primaryantibodiesab1378671.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)](https://www.abcam.com/ps/products/219/ab219372/Images/ab219372-291606-ab137867IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)This IHC data was generated using the same anti-MMP9 antibody clone, EP1255Y, in a different buffer formulation (cat# ab137867).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling MMP9 with purified ab137867 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Datasheets and documents
References
This product has been referenced in:
- Duan Y et al. Targeted silencing of CXCR4 inhibits epithelial-mesenchymal transition in oral squamous cell carcinoma. Oncol Lett 12:2055-2061 (2016). WB . Read more (PubMed: 27602138) »
- Xiao H et al. TAZ regulates cell proliferation and epithelial-mesenchymal transition of human hepatocellular carcinoma. Cancer Sci 106:151-9 (2015). Human . Read more (PubMed: 25495189) »
