Recombinant
RabMAb

Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)

Submit an Abreview Submit a Question Specific References (8)

Overview

  • Product name
    Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free
    See all MMP9 primary antibodies
  • Description
    Rabbit monoclonal [EP1255Y] to MMP9 - Low endotoxin, Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Dog, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MMP9 (C terminal).

  • Positive control
    • WB: U937 cell lysate. IHC-P: Human tonsil and lymphoma tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab219372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 78 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function
      May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
      -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
    • Tissue specificity
      Produced by normal alveolar macrophages and granulocytes.
    • Involvement in disease
      Intervertebral disc disease
      Metaphyseal anadysplasia 2
    • Sequence similarities
      Belongs to the peptidase M10A family.
      Contains 3 fibronectin type-II domains.
      Contains 4 hemopexin repeats.
    • Domain
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications
      Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
      N- and O-glycosylated.
    • Cellular localization
      Secreted, extracellular space, extracellular matrix.
    • Information by UniProt
    • Database links
      • Alternative names
        • 82 kDa matrix metalloproteinase-9 antibody
        • 92 kDa gelatinase antibody
        • 92 kDa type IV collagenase antibody
        • CLG 4B antibody
        • CLG4B antibody
        • Collagenase Type 4 beta antibody
        • Collagenase type IV 92 KD antibody
        • EC 3.4.24.35 antibody
        • Gelatinase 92 KD antibody
        • Gelatinase B antibody
        • Gelatinase beta antibody
        • GelatinaseB antibody
        • GELB antibody
        • Macrophage gelatinase antibody
        • MANDP2 antibody
        • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
        • Matrix Metalloproteinase 9 antibody
        • MMP 9 antibody
        • MMP-9 antibody
        • MMP9 antibody
        • MMP9_HUMAN antibody
        • Type V collagenase antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)

        This IHC data was generated using the same anti-MMP9 antibody clone, EP1255Y, in a different buffer formulation (cat# ab137867).

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling MMP9 with purified ab137867 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      References

      This product has been referenced in:
      • Duan Y  et al. Targeted silencing of CXCR4 inhibits epithelial-mesenchymal transition in oral squamous cell carcinoma. Oncol Lett 12:2055-2061 (2016). WB . Read more (PubMed: 27602138) »
      • Xiao H  et al. TAZ regulates cell proliferation and epithelial-mesenchymal transition of human hepatocellular carcinoma. Cancer Sci 106:151-9 (2015). Human . Read more (PubMed: 25495189) »

      See all 8 Publications for this product

      Publishing research using ab219372? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab219372.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

      Sign up