Recombinant Anti-MMP9 antibody [EPR22140-154] (ab228402)
![Western blot - Anti-MMP9 antibody [EPR22140-154] (ab228402)](https://www.abcam.com/ps/products/228/ab228402/Images/ab228402-315231-anti-mmp9-antibody-epr22140-154-western-blot.jpg "Western blot - Anti-MMP9 antibody [EPR22140-154] (ab228402)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22140-154] to MMP9
- Suitable for: IP, IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-MMP9 antibody [EPR22140-154]
See all MMP9 primary antibodies -
Description
Rabbit monoclonal [EPR22140-154] to MMP9 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse lung lysate; Rat lung and spleen lysates. IHC-P: Mouse spleen and lung tissues; Rat spleen tissue. IHC-Fr: Mouse lung tissue. IP: Mouse lung lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22140-154 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/30.
|
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| IHC-P | (1) |
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| IHC-Fr |
1/100.
|
|
| WB | (1) |
1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 81 kDa).
|
| Notes |
|---|
|
IP
1/30. |
|
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IHC-Fr
1/100. |
|
WB
1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 81 kDa). |
Target
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Function
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. -
Tissue specificity
Produced by normal alveolar macrophages and granulocytes. -
Involvement in disease
Intervertebral disc disease
Metaphyseal anadysplasia 2 -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsProcessing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 17395 Mouse
- Entrez Gene: 81687 Rat
- SwissProt: P41245 Mouse
- SwissProt: P50282 Rat
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Alternative names
- 82 kDa matrix metalloproteinase-9 antibody
- 92 kDa gelatinase antibody
- 92 kDa type IV collagenase antibody
see all
Images
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Western blot - Anti-MMP9 antibody [EPR22140-154] (ab228402)All lanes : Anti-MMP9 antibody [EPR22140-154] (ab228402) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Rat lung lysate
Lane 3 : Rat spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 81 kDa
Observed band size: 92 kDa why is the actual band size different from the predicted?Exposure times : Lanes 1-2: 3 minutes; Lane 3: 26 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
MMP9 is a glycoprotein (PMID: 29329315). The 92 kDa band likely represents the zymogen prior to activating cleavage of the pro-peptide (PMID: 7688350; PMID: 12901881).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)](https://www.abcam.com/ps/products/228/ab228402/Images/ab228402-315234-anti-mmp9-antibody-epr22140-154-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling MMP9 with ab228402 at 1/5,000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on polymorphonuclear leukocytes of mouse spleen (PMID: 28775117). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Heat-mediated antigen retrieval using citrate buffer (pH 6.0).
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![Immunohistochemistry (Frozen sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)](https://www.abcam.com/ps/products/228/ab228402/Images/ab228402-315232-anti-mmp9-antibody-epr22140-154-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling MMP9 with ab228402 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution (green). Positive staining on sporadic cells (macrophage-like) in mouse lung tissue is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution.
Heat-mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).
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![Immunoprecipitation - Anti-MMP9 antibody [EPR22140-154] (ab228402)](https://www.abcam.com/ps/products/228/ab228402/Images/ab228402-315233-anti-mmp9-antibody-epr22140-154-immunoprecipitation.jpg)
Immunoprecipitation - Anti-MMP9 antibody [EPR22140-154] (ab228402)MMP9 was immunoprecipitated from 0.35 mg of mouse brain lysate with ab228402 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab228402 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5,000 dilution.
Lane 1: Mouse lung lysate 10 µg (Input).
Lane 2: ab228402 IP in mouse lung lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab228402 in mouse lung lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 20 seconds.
The blot was developed using a high sensitivity ECL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)](https://www.abcam.com/ps/products/228/ab228402/Images/ab228402-315235-anti-mmp9-antibody-epr22140-154-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling MMP9 with ab228402 at 1/5,000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on polymorphonuclear leukocytes of mouse lung (PMID: 28775117). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Heat-mediated antigen retrieval using citrate buffer (pH 6.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)](https://www.abcam.com/ps/products/228/ab228402/Images/ab228402-315236-anti-mmp9-antibody-epr22140-154-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling MMP9 with ab228402 at 1/5,000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on polymorphonuclear leukocytes of rat spleen (PMID: 28775117). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Heat-mediated antigen retrieval using citrate buffer (pH 6.0).
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![Anti-MMP9 antibody [EPR22140-154] (ab228402)](https://www.abcam.com/ps/products/228/ab228402/Images/ab228402-7-benefits-of-recombinant-antibodies.png)
Anti-MMP9 antibody [EPR22140-154] (ab228402)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab228402 has been referenced in 5 publications.
- Ding X et al. Knockdown of Delta-like 3 restricts lipopolysaccharide-induced inflammation, migration and invasion of A2058 melanoma cells via blocking Twist1-mediated epithelial-mesenchymal transition. Life Sci 226:149-155 (2019). PubMed: 30981764
- He C et al. CircLMNB1 promotes colorectal cancer by regulating cell proliferation, apoptosis and epithelial-mesenchymal transition. Onco Targets Ther 12:6349-6359 (2019). PubMed: 31496737
- Wang Q et al. KLF4 overexpression decreases the viability, invasion and migration of papillary thyroid cancer cells. Exp Ther Med 18:3493-3501 (2019). PubMed: 31602225
- Wang F et al. Degradation of CCNB1 mediated by APC11 through UBA52 ubiquitination promotes cell cycle progression and proliferation of non-small cell lung cancer cells. Am J Transl Res 11:7166-7185 (2019). PubMed: 31814919
- Yang L et al. LncRNA Tug1 involves in the pulmonary vascular remodeling in mice with hypoxic pulmonary hypertension via the microRNA-374c-mediated Foxc1. Life Sci 237:116769 (2019). PubMed: 31422096