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    mmp9-antibody-epr22140-154-ab228402.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP
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RecombinantRabMAb

Recombinant Anti-MMP9 antibody [EPR22140-154] (ab228402)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (4) Submit a question References (19)

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Western blot - Anti-MMP9 antibody [EPR22140-154] (ab228402)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)
  • Immunohistochemistry (Frozen sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)
  • Immunoprecipitation - Anti-MMP9 antibody [EPR22140-154] (ab228402)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)
  • Anti-MMP9 antibody [EPR22140-154] (ab228402)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22140-154] to MMP9
  • Suitable for: IP, IHC-P, IHC-Fr, WB
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

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Human MMP9 ELISA Kit (ab246539)
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Recombinant Human MMP9 protein (Proenzyme) (ab82955)
Primary
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Anti-MMP2 antibody [EPR1184] (ab92536)

View more associated products

Overview

  • Product name

    Anti-MMP9 antibody [EPR22140-154]
    See all MMP9 primary antibodies
  • Description

    Rabbit monoclonal [EPR22140-154] to MMP9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse lung lysate; Rat lung and spleen lysates. IHC-P: Mouse spleen and lung tissues; Rat spleen tissue. IHC-Fr: Mouse lung tissue. IP: Mouse lung lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22140-154
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Matrix Metalloproteinases
    • MMP
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • MMP
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • ECM enzymes
    • MMPs
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MMPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • MMPs
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • MMPs
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other
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    • Neuroscience
    • Diseases
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-MMP9 antibody [EPR22140-154] - BSA and Azide free (ab237782)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human MMP9 protein (Proenzyme) (ab82955)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab228402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/30.
IHC-P (1)
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr (1)
1/100.
WB (2)
1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 81 kDa).
Notes
IP
1/30.
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr
1/100.
WB
1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 81 kDa).

Target

  • Function

    May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • Tissue specificity

    Produced by normal alveolar macrophages and granulocytes.
  • Involvement in disease

    Intervertebral disc disease
    Metaphyseal anadysplasia 2
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix.
  • Target information above from: UniProt accession P14780 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 17395 Mouse
    • Entrez Gene: 81687 Rat
    • SwissProt: P41245 Mouse
    • SwissProt: P50282 Rat
    • Alternative names

      • 82 kDa matrix metalloproteinase-9 antibody
      • 92 kDa gelatinase antibody
      • 92 kDa type IV collagenase antibody
      • CLG 4B antibody
      • CLG4B antibody
      • Collagenase Type 4 beta antibody
      • Collagenase type IV 92 KD antibody
      • EC 3.4.24.35 antibody
      • Gelatinase 92 KD antibody
      • Gelatinase B antibody
      • Gelatinase beta antibody
      • GelatinaseB antibody
      • GELB antibody
      • Macrophage gelatinase antibody
      • MANDP2 antibody
      • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
      • Matrix Metalloproteinase 9 antibody
      • MMP 9 antibody
      • MMP-9 antibody
      • MMP9 antibody
      • MMP9_HUMAN antibody
      • Type V collagenase antibody
      see all

    Images

    • Western blot - Anti-MMP9 antibody [EPR22140-154] (ab228402)
      Western blot - Anti-MMP9 antibody [EPR22140-154] (ab228402)
      All lanes : Anti-MMP9 antibody [EPR22140-154] (ab228402) at 1/1000 dilution

      Lane 1 : Mouse lung lysate
      Lane 2 : Rat lung lysate
      Lane 3 : Rat spleen lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 81 kDa
      Observed band size: 92 kDa why is the actual band size different from the predicted?



      Exposure times : Lanes 1-2: 3 minutes; Lane 3: 26 seconds.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      MMP9 is a glycoprotein (PMID: 29329315). The 92 kDa band likely represents the zymogen prior to activating cleavage of the pro-peptide (PMID: 7688350; PMID: 12901881).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling MMP9 with ab228402 at 1/5,000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on polymorphonuclear leukocytes of mouse spleen (PMID: 28775117). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Heat-mediated antigen retrieval using citrate buffer (pH 6.0).

    • Immunohistochemistry (Frozen sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)
      Immunohistochemistry (Frozen sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling MMP9 with ab228402  at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution (green). Positive staining on sporadic cells (macrophage-like) in mouse lung tissue is observed.

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution.

      Heat-mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunoprecipitation - Anti-MMP9 antibody [EPR22140-154] (ab228402)
      Immunoprecipitation - Anti-MMP9 antibody [EPR22140-154] (ab228402)

      MMP9 was immunoprecipitated from 0.35 mg of mouse brain lysate with ab228402 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab228402 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5,000 dilution.

      Lane 1: Mouse lung lysate 10 µg (Input). 

      Lane 2: ab228402 IP in mouse lung lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab228402 in mouse lung lysate.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 20 seconds.

      The blot was developed using a high sensitivity ECL.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)

      Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling MMP9 with ab228402 at 1/5,000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on polymorphonuclear leukocytes of mouse lung (PMID: 28775117). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Heat-mediated antigen retrieval using citrate buffer (pH 6.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EPR22140-154] (ab228402)

      Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling MMP9 with ab228402 at 1/5,000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on polymorphonuclear leukocytes of rat spleen (PMID: 28775117). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Heat-mediated antigen retrieval using citrate buffer (pH 6.0).

    • Anti-MMP9 antibody [EPR22140-154] (ab228402)
      Anti-MMP9 antibody [EPR22140-154] (ab228402)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (19)

    Publishing research using ab228402? Please let us know so that we can cite the reference in this datasheet.

    ab228402 has been referenced in 19 publications.

    • Zhou S  et al. MiR-370-3p inhibits the development of human endometriosis by downregulating EDN1 expression in endometrial stromal cells. Cell Biol Int 45:1183-1190 (2021). PubMed: 33470475
    • Li H  et al. Exosomes containing miR-451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injury. CNS Neurosci Ther 27:564-576 (2021). PubMed: 33533575
    • Zheng F  et al. KCa3.1 Inhibition of Macrophages Suppresses Inflammatory Response Leading to Endothelial Damage in a Cell Model of Kawasaki Disease. J Inflamm Res 14:719-735 (2021). PubMed: 33727847
    • Qin K  et al. Circular RNA circ-ARFIP2 regulates proliferation, migration and invasion in human vascular smooth muscle cells via miR-338-3p-dependent modulation of KDR. Metab Brain Dis 36:1277-1288 (2021). PubMed: 33837886
    • Feng Y  et al. FOXK1 plays an oncogenic role in the progression of hilar cholangiocarcinoma. Mol Med Rep 23:N/A (2021). PubMed: 33300075
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-4 of 4 Abreviews

    Western blot abreview for Anti-MMP9 antibody [EPR22140-154]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (colon)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris)
    Loading amount
    15 µg
    Specification
    colon
    Blocking step
    Commercial blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 01 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-MMP9 antibody [EPR22140-154]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Skeletal muscle)
    Permeabilization
    Yes - TritonX (0.1%)
    Specification
    Skeletal muscle
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 28 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MMP9 antibody [EPR22140-154]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate pH 6.0
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 07 2020

    Western blot abreview for Anti-MMP9 antibody [EPR22140-154]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (trophoblast cells)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    300000 cells
    Specification
    trophoblast cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 22 2019

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