Anti-MMP9 antibody [MMP9/2025R] (ab236494)

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Overview

  • Product name

    Anti-MMP9 antibody [MMP9/2025R]
    See all MMP9 primary antibodies

  • Description

    Rabbit monoclonal [MMP9/2025R] to MMP9

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human MMP9.
    Database link: P14780

  • Positive control

    • IHC-P: Human spleen tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab236494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml.

Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 minutes at RT.

                        

Target

  • Function

    May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.

  • Tissue specificity

    Produced by normal alveolar macrophages and granulocytes.

  • Involvement in disease

    Intervertebral disc disease
    Metaphyseal anadysplasia 2

  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.

  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

  • Post-translational
    modifications

    Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.

  • Cellular localization

    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt

  • Database links

    • Alternative names

      • 82 kDa matrix metalloproteinase-9 antibody
      • 92 kDa gelatinase antibody
      • 92 kDa type IV collagenase antibody
      • CLG 4B antibody
      • CLG4B antibody
      • Collagenase Type 4 beta antibody
      • Collagenase type IV 92 KD antibody
      • EC 3.4.24.35 antibody
      • Gelatinase 92 KD antibody
      • Gelatinase B antibody
      • Gelatinase beta antibody
      • GelatinaseB antibody
      • GELB antibody
      • Macrophage gelatinase antibody
      • MANDP2 antibody
      • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
      • Matrix Metalloproteinase 9 antibody
      • MMP 9 antibody
      • MMP-9 antibody
      • MMP9 antibody
      • MMP9_HUMAN antibody
      • Type V collagenase antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [MMP9/2025R] (ab236494)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [MMP9/2025R] (ab236494)

      Formalin-fixed, paraffin-embedded human spleen tissue stained for MMP9 using ab236494 at 1 μg/ml in immunohistochemical analysis.

    • SDS-PAGE - Anti-MMP9 antibody [MMP9/2025R] (ab236494)
      SDS-PAGE - Anti-MMP9 antibody [MMP9/2025R] (ab236494)

      SDS-PAGE analysis of ab236494 in non-reduced (NR) or reduced (R) conditions.

    References

    ab236494 has not yet been referenced specifically in any publications.

    Publishing research using ab236494? Please let us know so that we can cite the reference in this datasheet.

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