Anti-MMP9 antibody [MMP9/2025R] (ab236494)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [MMP9/2025R] (ab236494)](https://www.abcam.com/ps/products/236/ab236494/Images/ab236494-312859-anti-mmp9-antibody-mmp9-2025r-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [MMP9/2025R] (ab236494)")
Key features and details
- Rabbit monoclonal [MMP9/2025R] to MMP9
- Suitable for: IHC-P, SDS-PAGE
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MMP9 antibody [MMP9/2025R]
See all MMP9 primary antibodies -
Description
Rabbit monoclonal [MMP9/2025R] to MMP9 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, SDS-PAGEmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human MMP9.
Database link: P14780 -
Positive control
- IHC-P: Human spleen tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
MMP9/2025R -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 0.5 - 1 µg/ml.
Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 minutes at RT.
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| SDS-PAGE |
Use at an assay dependent concentration.
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| Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 minutes at RT.
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SDS-PAGE
Use at an assay dependent concentration. |
Target
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Function
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. -
Tissue specificity
Produced by normal alveolar macrophages and granulocytes. -
Involvement in disease
Intervertebral disc disease
Metaphyseal anadysplasia 2 -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsProcessing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4318 Human
- Omim: 120361 Human
- SwissProt: P14780 Human
- Unigene: 297413 Human
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Alternative names
- 82 kDa matrix metalloproteinase-9 antibody
- 92 kDa gelatinase antibody
- 92 kDa type IV collagenase antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [MMP9/2025R] (ab236494)
Formalin-fixed, paraffin-embedded human spleen tissue stained for MMP9 using ab236494 at 1 μg/ml in immunohistochemical analysis.
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![SDS-PAGE - Anti-MMP9 antibody [MMP9/2025R] (ab236494)](https://www.abcam.com/ps/products/236/ab236494/Images/ab236494-312860-anti-mmp9-antibody-mmp9-2025r-sds-page.jpg)
SDS-PAGE - Anti-MMP9 antibody [MMP9/2025R] (ab236494)SDS-PAGE analysis of ab236494 in non-reduced (NR) or reduced (R) conditions.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab236494 has been referenced in 1 publication.
- Wang P et al. Psychological Stress Up-Regulates CD147 Expression Through Beta-Arrestin1/ERK to Promote Proliferation and Invasiveness of Glioma Cells. Front Oncol 10:571181 (2020). PubMed: 33178600
